Mechanisms of expression of neuroendocrine phenotype in the mammary gland

乳腺神经内分泌表型的表达机制

• 批准号: 11670194
• 负责人: UMEMURA Shinobu
• 金额: $ 1.86万
• 依托单位: Tokai University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670194/
• 关键词: mammary gland; neuroendocrine; chromogranin; RT-PCR; breast cancer; 神経内分泌顆粒; Cg mRNA; RT-PCR法; 乳腺

The aim of the present study is to clarify whether chromogranin/secretogranin mRNA expresses in the normal mammary gland and mammary gland tumors. The mammary gland used was under the condition of resting, pregnancy, lactation and involution. The mammary gland tumors were fibroadenoma and adenocarcinoma. Results were (1) No apparent neuroendocrine granules were identified by electronmicroscopical study. (2) CgA, SgI and SgII mRNA expression was identified in the late pregnancy. CgA mRNA expression was additionally found in the early phase of the lactation. (3) CgA and SgII mRNA expression were identified in both of the rat mammary gland tumors, although SgI mRNA expression was not detected. (4) CgA, SgI and SgII mRNA were not induced by treatment of estradiol (5, 50, 500, 1000 μg/kg/week, 1-4 weeks). (5) At the same time, PRL mRNA was examined. PRL mRNA was expressed through mid pregnancy to lacatation. (6) PRL mRNA was also identified in mammary gland tumors (fibroadenoma and adenocarcinoma).

本研究的目的是阐明嗜铬粒蛋白/分泌粒蛋白mRNA在正常乳腺和乳腺肿瘤中的表达。所用乳腺处于静止、妊娠、哺乳和退化状态。乳腺肿瘤为纤维腺瘤和腺癌。结果：（1）电镜下未见明显的神经内分泌颗粒。(2)CgA、SgI和SgII mRNA在妊娠晚期表达。此外，CgA mRNA的表达被发现在泌乳早期。(3)CgA和SgII mRNA的表达被确定在这两个大鼠乳腺肿瘤，虽然SgI mRNA的表达未被检测到。(4)雌二醇（5、50、500、1000 μg/kg/wk，1-4周）处理对CgA、SgI和SgII mRNA无诱导作用。(5)同时检测PRL mRNA的表达。PRL mRNA在妊娠中期至泌乳期均有表达。(6)在乳腺肿瘤（纤维腺瘤和腺癌）中也发现了PRL mRNA。

项目成果

梅村しのぶ 他: "ラット乳腺における神経内分泌的形質発現について"乳癌基礎研究. 8. 1-4 (1999)

Shinobu Umemura 等：“大鼠乳腺中的神经内分泌表达”乳腺癌基础研究。8. 1-4 (1999)。

H-S.Jin, S.Umemura, et al.: "Alterations of myoepithelial cells in the rat mammary gland during pregnancy, lactation and involution, and after estradiol treatment."Pathol Int. 50. 384-391 (2000)

H-S.Jin、S.Umemura 等人：“妊娠、哺乳、复旧期间以及雌二醇治疗后大鼠乳腺肌上皮细胞的变化。”Pathol Int。

T.Iwasaka, et al.: "Lack of prolactin (PRL) mRNA expression in estrogen-treated rat mammary glands."J.Toxicol.Pathol.. 13. 151-157 (2000)

T.Iwasaka 等人：“雌激素处理的大鼠乳腺中缺乏催乳素 (PRL) mRNA 表达。”J.Toxicol.Pathol.. 13. 151-157 (2000)

T.Iwasaka, S.Umemura, et al.: "Lack of prolactin (PRL) mRNA expression in estrogen-treated rat mammary glands."J Toxicol Pathol. 13. 151-157 (2000)

T.Iwasaka、S.Umemura 等人：“雌激素处理的大鼠乳腺中缺乏催乳素 (PRL) mRNA 表达。”J Toxicol Pathol。

S.Umemura, et al.: "Neuroendocrine differentiation in the rat mammary gland [in Japanese]."Nyuugannkisokennkyuu. 8. 1-4 (1999)

S.Umemura 等人：“大鼠乳腺的神经内分泌分化[日语]。”Nyuugannkisokennkyuu。