Study on the Nasal T/NK cell lymphoma

鼻T/自然杀伤（NK）细胞淋巴瘤的研究一直受到其引起坏死倾向的阻碍。因此，建立这种肿瘤细胞系似乎是有价值的。在本研究中，我们旨在从该肿瘤的原发病变中建立细胞系，并通过高剂量重组白细胞介素2成功获得了两个新的EBV阳性细胞系SNK-6和SNT-8。流式细胞术显示，SNK-6具有nk细胞表型CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-，而SNT-8为CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+，与原肿瘤免疫表型一致，且细胞系具有nk细胞表型。与原肿瘤相同的EBV单克隆。因此，细胞系从形成原发病变的细胞发展而来。基因型分析显示，SNK-6具有未重排的TCR和免疫球蛋白重链基因，支持SNK-6属于nk细胞系的结论。另一方面，SNT-8重组了TCR β-、γ-和δ-链基因，并结合其表型证明了SNT-8是一个γδ t细胞系。这是首次从鼻部T/NK细胞淋巴瘤原发病变中建立细胞系的报道，结果表明该肿瘤至少存在NK-和γδ T细胞两种细胞系。此外，有研究表明，这些谱系的鼻T/NK细胞淋巴瘤可能属于相同的临床病理实体，因为这两种类型的病例具有共同的临床和组织病理特征。

Studies on nasal T/natural killer (NK) cell lymphoma have been hampered by its tendency to cause necrosis. Thus, the establishment of cell lines of this neoplasm would seem to be valuable. In the present study, we aimed to establish cell lines from primary lesions of this tumor, and successfully obtained two novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high dose recombinant interleukin 2. Flow cytometry showed that SNK-6 had an NK-cell phenotype, CD3^+ 4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-, whereas SNT-8 was CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+, These were consistent with immunophenotypes of their original tumors, and the cell lines had. Monoclonal EBV clones identical to ones in their original tumors. Thus, the cell lines developed from cells forming the primary, lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6 was of NK-cell lineage. On the other hand, SNT-8 had rearranged TCR β-,γ-, and δ-chain genes, and together with its phenotype, SNT-8 proved to be a γδ T-cell line. This is the first report of the establishment of cell lines from primary lesions of nasal T/NK cell lymphomas, and the results demonstrated that there are at least two lineages, NK- and γδ T-cell, in this neoplasm. Moreover, it has been suggested that nasal T/NK cell lymphomas of these lineages may belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features.