Study on the Nasal T/NK cell lymphoma

鼻T/自然杀伤（NK）细胞淋巴瘤的研究一直受到阻碍，其倾向于导致坏死。因此，建立这种肿瘤的细胞系似乎是有价值的。在本研究中，我们的目的是建立细胞系从原发灶的这种肿瘤，并成功地获得了两个新的EB病毒（EBV）阳性细胞株，SNK-6和SNT-8，通过高剂量的重组白细胞介素2。流式细胞术显示SNK-6具有NK细胞表型，CD3 ^+4 ^-8 ^-19 ^-56 ^+ TCR α/β ^-γ/δ ^-，而SNT-8为CD3 ^+4 ^-8 ^-19 ^-56 ^+ TCR α/β ^-γ/δ ^+，这些与其原始肿瘤的免疫表型一致，并且细胞系具有。单克隆EBV克隆与其原始肿瘤中的克隆相同。因此，细胞系从形成原发性病变的细胞发育而来。基因型分析显示SNK-6具有未重排的TCR和免疫球蛋白重链基因，支持SNK-6具有NK细胞谱系的结论。另一方面，SNT-8具有重排的TCR β-、γ-和δ-链基因，并且连同其表型，SNT-8被证明是γ δ T细胞系。本文首次从鼻T/NK细胞淋巴瘤的原发灶中建立了细胞系，结果表明鼻T/NK细胞淋巴瘤中至少存在NK和γ δ T细胞两种细胞系。此外，有人建议，鼻T/NK细胞淋巴瘤的这些谱系可能属于同一临床病理实体，因为这两种类型的情况下，共享共同的临床和组织病理学特征。

Studies on nasal T/natural killer (NK) cell lymphoma have been hampered by its tendency to cause necrosis. Thus, the establishment of cell lines of this neoplasm would seem to be valuable. In the present study, we aimed to establish cell lines from primary lesions of this tumor, and successfully obtained two novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high dose recombinant interleukin 2. Flow cytometry showed that SNK-6 had an NK-cell phenotype, CD3^+ 4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-, whereas SNT-8 was CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+, These were consistent with immunophenotypes of their original tumors, and the cell lines had. Monoclonal EBV clones identical to ones in their original tumors. Thus, the cell lines developed from cells forming the primary, lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6 was of NK-cell lineage. On the other hand, SNT-8 had rearranged TCR β-,γ-, and δ-chain genes, and together with its phenotype, SNT-8 proved to be a γδ T-cell line. This is the first report of the establishment of cell lines from primary lesions of nasal T/NK cell lymphomas, and the results demonstrated that there are at least two lineages, NK- and γδ T-cell, in this neoplasm. Moreover, it has been suggested that nasal T/NK cell lymphomas of these lineages may belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features.