Study on the Nasal T/NK cell lymphoma

鼻腔T/自然杀伤(NK)细胞淋巴瘤的研究因其易导致坏死而受到阻碍。因此，建立该肿瘤的细胞系似乎是有价值的。在本研究中，我们从肿瘤的原发灶建立了细胞系，并利用高剂量重组白介素2成功地获得了两个新的EB病毒阳性细胞株SNK-6和SNT-8。流式细胞仪检测显示SNK-6具有NK细胞表型，CD3^+4^-8^-19^-56^+TCRα/β^-γ/δ^-，而SNT-8是CD3^+4-8^-19^-56^+TCRα/β^-γ/δ^+，这与其原始肿瘤的免疫表型是一致的。与其原始肿瘤中的克隆相同的单克隆性EBV克隆。因此，细胞系是从形成原发病变的细胞发展而来的。基因分型分析表明，SNK-6具有未重排的TCR和免疫球蛋白重链基因，支持SNK-6属于NK细胞系的结论。另一方面，SNT-8对TcR、β-，γ-和δ链基因进行了重排，结合其表型，证明SNT-8为γδT细胞系。这是首次从鼻部T/NK细胞淋巴瘤的原发病变建立细胞系，结果表明该肿瘤中至少有两种细胞系，即NK细胞和γδT细胞。此外，这些家系的鼻部T/NK细胞淋巴瘤可能属于同一临床病理实体，因为这两种类型的病例具有共同的临床和组织病理学特征。

Studies on nasal T/natural killer (NK) cell lymphoma have been hampered by its tendency to cause necrosis. Thus, the establishment of cell lines of this neoplasm would seem to be valuable. In the present study, we aimed to establish cell lines from primary lesions of this tumor, and successfully obtained two novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high dose recombinant interleukin 2. Flow cytometry showed that SNK-6 had an NK-cell phenotype, CD3^+ 4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-, whereas SNT-8 was CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+, These were consistent with immunophenotypes of their original tumors, and the cell lines had. Monoclonal EBV clones identical to ones in their original tumors. Thus, the cell lines developed from cells forming the primary, lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6 was of NK-cell lineage. On the other hand, SNT-8 had rearranged TCR β-,γ-, and δ-chain genes, and together with its phenotype, SNT-8 proved to be a γδ T-cell line. This is the first report of the establishment of cell lines from primary lesions of nasal T/NK cell lymphomas, and the results demonstrated that there are at least two lineages, NK- and γδ T-cell, in this neoplasm. Moreover, it has been suggested that nasal T/NK cell lymphomas of these lineages may belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features.