Study on the Nasal T/NK cell lymphoma

鼻T/NK细胞淋巴瘤的研究

• 批准号: 11670207
• 负责人: SHIMIZU Norio
• 金额: $ 1.98万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670207/
• 关键词: NASAL T; NK LYMPHOMA; LYMPHOMA; gdT-LYMPHOCYTE; DIAGNOSIS; CELL CULTURE; NK-CELL; 進行性鼻懐疸; γδTリンパ球

Studies on nasal T/natural killer (NK) cell lymphoma have been hampered by its tendency to cause necrosis. Thus, the establishment of cell lines of this neoplasm would seem to be valuable. In the present study, we aimed to establish cell lines from primary lesions of this tumor, and successfully obtained two novel Epstein-Barr virus (EBV)-positive cell lines, SNK-6 and SNT-8, by means of high dose recombinant interleukin 2. Flow cytometry showed that SNK-6 had an NK-cell phenotype, CD3^+ 4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-, whereas SNT-8 was CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+, These were consistent with immunophenotypes of their original tumors, and the cell lines had. Monoclonal EBV clones identical to ones in their original tumors. Thus, the cell lines developed from cells forming the primary, lesions. Genotypic analysis showed that SNK-6 had unrearranged TCR and immunoglobulin heavy-chain genes, supporting the conclusion that SNK-6 was of NK-cell lineage. On the other hand, SNT-8 had rearranged TCR β-,γ-, and δ-chain genes, and together with its phenotype, SNT-8 proved to be a γδ T-cell line. This is the first report of the establishment of cell lines from primary lesions of nasal T/NK cell lymphomas, and the results demonstrated that there are at least two lineages, NK- and γδ T-cell, in this neoplasm. Moreover, it has been suggested that nasal T/NK cell lymphomas of these lineages may belong to the same clinicopathologic entity because both types of cases shared common clinical and histopathologic features.

鼻 T/自然杀伤 (NK) 细胞淋巴瘤的研究因其导致坏死的倾向而受到阻碍。因此，建立这种肿瘤的细胞系似乎是有价值的。在本研究中，我们旨在从该肿瘤的原发灶建立细胞系，并通过高剂量重组白细胞介素2成功获得两种新型EB病毒（EBV）阳性细胞系SNK-6和SNT-8。流式细胞术显示SNK-6具有NK细胞表型CD3^+ 4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^-，而SNT-8是CD3^+4^- 8^- 19^- 56^+ TCRα/β^- γ/δ^+，这些与原始肿瘤的免疫表型一致，并且细胞系具有。单克隆 EBV 克隆与其原始肿瘤中的克隆相同。因此，细胞系由形成原发病变的细胞发育而来。基因型分析显示，SNK-6 具有未重排的 TCR 和免疫球蛋白重链基因，支持 SNK-6 属于 NK 细胞谱系的结论。另一方面，SNT-8重新排列了TCR β-、γ-和δ-链基因，结合其表型，SNT-8被证明是γδ T细胞系。这是首次报道鼻T/NK细胞淋巴瘤原发灶建立细胞系，结果表明该肿瘤中至少存在NK-和γδ T-细胞两种谱系。此外，有人认为这些谱系的鼻 T/NK 细胞淋巴瘤可能属于同一临床病理实体，因为两种类型的病例具有共同的临床和组织病理学特征。

项目成果

Nagata H., et al.: "Characterization of Novel Natural Killer (NK)-Cell and gamma/delta T-Cell Lines Established from Primary Lesions of Nasal T/NK Cell Lymphomas Associated with the Epstein-Barr Virus."Blood . 97(3). 708-713 (2001)

Nagata H.等人：“从与 Epstein-Barr 病毒相关的鼻 T/NK 细胞淋巴瘤原发性病变中建立的新型自然杀伤 (NK) 细胞和 γ/δ T 细胞系的特征。”血液。

H, Nagata., T, Numata., A, Konno., I, Mikata., K, Kurasawa., S, Hara., M, Nishimura., K, Yamamoto., N, Shimizu.: "Presence of natural killer-cell clones with variable porliterative capacity in chronic active Epstein-Bart virus infection"Pathology Intermat

H、永田、T、沼田、A、绀野、I、三方、K、仓泽、S、原、M、西村、K、山本、N、清水：“自然杀手的存在

A, Nakajima., Y, Kuriyama., N, Shimizu., J, H, Ohyashiki., K, Mukai., K, Ohyashiki.: "Detection of Epstein-Barr virus in a pyothorax-associated lymphoma with T-cell phenotype."Haematologica. 87(8). 708-713 (2002)

A，Nakajima.，Y，Kuriyama.，N，Shimizu.，J，H，Ohyashiki.，K，Mukai.，K，Ohyashiki.：“在具有 T 细胞表型的脓胸相关淋巴瘤中检测 Epstein-Barr 病毒