Gene cloning of new factors from hepatic stellate cells, production of its antibody and functional analysis

肝星状细胞新因子基因克隆、抗体制备及功能分析

• 批准号: 11670215
• 负责人: KITAMURA Yukisato
• 金额: $ 1.92万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670215/
• 关键词: hepatic stellate cell; simian virus 40; temperature-sensitive; cell line; growth; differentiation

Hepatic stellate cell (HSC) is one type of perisinusoidal cells in the liver and has many unique characteristics. HSC produce various cytokines and growth factors, and play an important role on the various pathophysiological state of liver diseases. Investigators carried out this project for studying the gene mechanism of HSC growth and differentiation, and for gene cloning of new factors which play an important role on HSC growth and differentiation.Investigators selected and cloned a specific gene which expressed under two conditions, the first was that HSC expressed large T antigen (T-ag) and the second was that HSC did not express T-ag, using temperature-sensitive mutant of simian virus 40. The specific genes which were thought to express in an active phenotype of HSC and in a quiescent phenotype of HSC were sequenced. It was suggested that these genes played an important role on the pathophysiological state of chronic hepatitis and liver cirrhosis in human liver tissue. Investigators also studied the signal transduction in HSC about fibrogenesis and this study is now being submitted.This project is now carrying out for an antibody production of new factors from HSC and for gene analysis. It is the final aim that the gene therapy against fibrosis is established.

肝星状细胞（HSC）是肝脏窦周细胞的一种，具有许多独特的细胞学特征。HSC产生多种细胞因子和生长因子，在肝脏疾病的各种病理生理状态中发挥重要作用。为了研究HSC生长和分化的基因机制，克隆对HSC生长和分化起重要作用的新因子，本研究选择并克隆了一个在两种条件下表达的特异性基因，第一种是HSC表达大T抗原（T-ag），第二种是HSC不表达T-ag，使用猿猴病毒40的温度敏感突变体。对认为在HSC的活跃表型和静止表型中表达的特定基因进行测序。提示这些基因在慢性肝炎和肝硬化的病理生理状态中起重要作用。研究人员还研究了HSC中关于纤维化的信号转导，这项研究目前正在提交。该项目目前正在进行HSC新因子的抗体生产和基因分析。建立抗肝纤维化的基因治疗是最终目标。

项目成果

Noriko Miyake: "Absence of mutations in the β-catenin and adenomatous polyposis coli genes in papillary and follicular thyroid carcinomas"Pathology International. 51. 680-685 (2001)

Noriko Miyake：“乳头状和滤泡性甲状腺癌中 β-连环蛋白和腺瘤性息肉病基因不存在突变”《国际病理学》51. 680-685 (2001)。

Hiroshi Hosokawa: "Vascular endothelial cells that express dystroglycan are involued in angiogenesis"Journal of Cell Science. (in press).

Hiroshi Hosokawa：“表达肌营养不良聚糖的血管内皮细胞参与血管生成”《细胞科学杂志》。

Yukisato Kitamura: "A case of clear cell concinoma of the ovary with uncommon histologic components"Journal of the Okayama Singical Pathology Association. 36. 1-5 (1999)

Yukisato Kitamura：“一例具有不常见组织学成分的卵巢透明细胞癌”冈山病理学协会杂志。

Yukisato Kitamura et al.: "A case of clear cell carcinoma of the ovary with uncommon histologic components"The Journal of the Okayama Surgical Pathology Association. 36. 1-5 (1999)

Yukisato Kitamura 等人：“一例具有不常见组织学成分的卵巢透明细胞癌”冈山外科病理学协会杂志。

Satoshi Horie: "Infebifory effects of antisense oligenuclesticles on the expression of procollagen typeIII gene in mouse hepatic stellate cells ransformed by simian virus 40"Pathology International. 50. 937-944 (2000)

Satoshi Horie：“反义寡核对由猿猴病毒 40 转化的小鼠肝星状细胞中 III 型前胶原基因表达的感染效应”国际病理学。