ELEVATION OF CYSTATHIONINE γ-LYASE ACTIVITY IN THE SERUM OF RATS TREATED WITH A SINGLE DOSE OF CCL_4

单剂量 CCL_4 处理的大鼠血清中胱硫醚 γ-裂解酶活性的升高

• 批准号: 11670343
• 负责人: TAGUCHI Tetsuya
• 金额: $ 1.66万
• 依托单位: KOCHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670343/
• 关键词: cystathionine γ-lyase; carbon tetrachloride; hepatotoxicity; rats; γ-lyase; 尿中蛋白質

Cystathionine γ-lyase activity in the sera of rats subjected to experimental hepatotoxicity after intraperitoneal administration of carbon tetrachloride was measured and compared with activities of aspartate aminotransferase (GOT) and alanine aminotransferase (GPT), which have been clinically used for detecting liver damage. In the experimental subjects, serum levels of cystathionine γ-lyase showed a similar behavior to GOT and GPT, increasing markedly with respect to the controls after administration of carbon tetrachloride and reaching a maximum level at 24 hours. No such cystathionine γ-lyase activity was detected immunochemically in the control subjects. These data suggest that measurement of serum cystathionine γ-lyase activity could be used as a sensitive and specific marker of hepatic cytolysis. However, it remains necessary to confirm these results in different pathological situations affecting the human liver.

本文测定了四氯化碳（CCl_4）致肝损伤大鼠血清中胱硫醚γ-裂解酶（Cystathiopathy γ-lyase，CSH）活性，并与临床上检测肝损伤的谷草转氨酶（GOT）和谷丙转氨酶（GPT）活性进行了比较。在实验受试者中，血清胱硫醚γ-裂解酶水平表现出与GOT和GPT相似的行为，在给予四氯化碳后相对于对照组显著增加，并在24小时达到最高水平。在对照受试者中未检测到这种胱硫醚γ-裂解酶活性。提示血清胱硫醚γ-裂解酶活性测定可作为肝细胞溶解的敏感、特异性指标。然而，仍然有必要在影响人类肝脏的不同病理情况下确认这些结果。