Utilization of Flowcytometry for Accessment of Natural Immunity as a Body Defense Mechanism in Aged People

我们研究了老年人（65岁以上）的生活方式与自然免疫作为一种非特异性身体防御机制的相关性。在本研究中，我们着重于调查老年人的免疫功能，并利用结果数据来评估老年人的卫生保健。在为期3年的调查中，共有290人（65岁以上，男性116人，女性174人）居住在埼玉县Nagri市。我们测量了他们的免疫功能，如自然杀伤（NK）细胞活性，中性粒细胞的吞噬和活性氧产生，用流式细胞术分析淋巴细胞亚群（T细胞和B细胞）的数量，以及血清生化和尿液分析，此外还调查了他们的生活方式，如健康习惯、运动或体育活动、饮食、吸烟和饮酒习惯等。我们还讨论了哪种生活方式对保持老年人身体健康和免疫功能作为身体防御机制更重要。结果表明，中性粒细胞功能（吞噬活性和活性氧产生）相对容易受到生活方式的影响，即与饮食习惯、压力或道德考验显著相关，与男性握力和反射率也相关，但不显著。NK细胞（CD16+CD56+）和NKT细胞（CD3+CD56+）的数量也与饮食习惯或身体活动相关，但与压力或道德测试无关。淋巴细胞功能倾向于与男性良好的饮食或饮酒习惯有关，而与女性低BMI（非肥胖）有关。

We have studied on correlation of the life style of aged people (over 65 years old) to natural immunity as a non-specific body defense mechanism. In the present study, we focused to investigate the immune function of aged people and to utilize the resulted data for assessment of health care for aged people. The total number of subjects attended in the survey carried out for 3 years were 290 people (over 65-year old 116 male and 174 female) who lived in Nagri, Saitama prefecture. We measured their immunological function such as natural killer (NK) cell activity, phagocytosis and active oxygen production of neutrophiles, and population of lymphocyte subsets (T and B cells) analyzed with flowcytometry method along with serum biochemistry and urinalysis, in addition to questionnaire for their life style such as health practice, locomotion or physical activity, diet, smoking, and alcohol habits, and so on. We also discussed which life style is more important to maintain their good health and immune function as a body defense mechanism in aged people.As a result, it was shown that neutrophile function (phagocytic activity and active oxygen production) was comparatively susceptible to life style, that is, significantly correlated to dietary habit, stress or moral test, and also correlated, but not significant, to grasping power in men and reflectivity. The numbers of both NK (CD16+CD56+) and NKT cells (CD3+CD56+) also correlated to dietary habit or physical activity but not to stress nor moral test. Lymphocyte function tended to correlate to good dietary or alcohol habit in men and to low BMI (not obesity) in women.