Experimental and Clinicoepidemiological Study of Mechanism by which Chronic Beryllium Disease Develops

1. 豚鼠给药铍（Be）化合物致敏性的研究1)将豚鼠分为对照组、BeO给药组和BeO+BeSO_4给药组。采用Be特异性淋巴细胞刺激试验（Be- lt）研究致敏活性的差异。（结果）BeO给药组的Be-LT值明显高于对照组和BeO+BeSO_4给药组。2)将动物分为佐剂不给药组（BeSO_4溶液）、FCA联合组（BeSO_4+FCA溶液）和FIA联合组（BeSO_4+FIA溶液）。采用Be-LT研究致敏活性的差异。（结果）FCA联合用药组的Be-LT值明显高于非辅助用药组。（讨论）当观察Be给药组的Be- lt值变化时，BeO给药组的Be- lt值高于BeO+BeSO_4给药组。这表明，根据所使用的Be化合物的不同，腹腔注射Be化合物的免疫效应在某些情况下以加速方式起作用，在其他情况下以抑制方式起作用。与其他实验组相比，BeO给药组和FCA联合用药组的Be-LT值较高。这表明使用FCA气管内或皮内给药是有用的。2、Be对雌雄小鼠脾细胞存活率的影响利用小鼠脾细胞，我们研究了Be对免疫细胞的影响，特别是由于性别差异对免疫细胞存活率的影响。（结果）对照组（Be浓度为0 μ M）雌雄小鼠细胞存活率随孵育时间的延长而降低。雄性小鼠在1、10、100 μM时的细胞存活率与对照组相似。在100 μM和1000 μM时，与对照相比，其含量显著降低。雌性小鼠在1 μM和10 μM下的细胞存活率与对照组相似。在100 μM和1000 μM孵育24 h后，与对照相比有明显下降。（讨论）关于Be对雌雄小鼠、雌雄小鼠的影响差异，雌性小鼠的存活率下降要早于雄性小鼠。有趣的是，在本研究中，雌性小鼠脾细胞存活率的下降比雄性小鼠出现得更早。我们打算进一步研究这一现象。少

1. A Study on Sensitization of Guinea Pigs Administered Beryllium (Be) Compound1) The animals were divided into the control group, BeO administration group and BeO+BeSO_4 administration group. A Be specific lymphocyte stimulating test (Be-LT) was performed to study differences in the sensitizing activity.(Results) The BeO administration group showed a significantly high Be-LT value in comparison with the control group and BeO+BeSO_4 administration group.2) The animals were divided into the adjuvant non-administration group (BeSO_4 solution), FCA combined group (BeSO_4+FCA solution) and FIA combined group (BeSO_4+FIA solution). Be-LT was performed to study differences in the sensitizing activity.(Results) The FCA combined group showed a significantly high Be-LT value in comparison with the adjuvant non-administration group.(Discussion) When changes in the Be-LT value were examined in the Be administration group, the Be-LT value in the BeO administration group was high in comparison with … More that in the BeO+BeSO_4 administration group. This suggests that the imnunological effect of intraperitoneal administration of Be compounds works in an accelerating manner in some cases and in suppressing manner in others according to the difference in Be compounds used. Be-LT value in the BeO administration group and FCA combined group was high in comparison with that in the other experimental groups. This suggests that endotracheal administration or intracutaneous administration using FCA is useful.2, Influence of Be on the survival rate in splenic cells of male and female miceUsing mouse splenic cells, we studied the influence of Be on immunocytes, particularly the survival rate due to differences between the sexes.(Results) The cell survival rate in the male and female mice of the control group (Be concentration : 0 μ M) decreased with the lapse of incubation time. The cell survival rate at 1, 10, 100 μM in male mice showed a tendency similar to that in the control. At 100 and 1000 μM, it showed a significant decrease compared with the control. The cell survaval rate at 1 and 10 μM in female mice showed a tendency similar to that in the control. At 100 and 1000 μM, it showed a significant decrease compared with the control from the 24th hour of incubation.(Discussion) Regarding the difference in the influence of Be between male and female mice, of Be between male and female mice, the decline in the survival rate presented itself earlier in female mice than in male mice. Interestingly, the decrease in the splenic cell survival rate in female mice appeared earlier than that in male mice in this study. We intend to study this phenomenon further. Less