Experimental and Clinicoepidemiological Study of Mechanism by which Chronic Beryllium Disease Develops

1.铍化合物对豚鼠致敏作用的研究1)将豚鼠分为对照组、BeO组和BeO+BeSO_4组。结果BeO给药组的BE-LT值明显高于对照组和BeO+BeSO4给药组。2)动物分为非佐剂给药组(BeSO4溶液)、FCA联合组(BeSO4+FCA溶液)和FIA联合组(BeSO4+FIA溶液)。结果FCA联合用药组BE-LT值明显高于非给药佐剂组(P<0.05)，BE给药组BE-LT值明显高于…给药组。BeO+BaSO4给药组作用更明显。提示Be化合物的免疫效应在某些情况下是加速的，而在另一些情况下则是抑制作用，这与使用Be化合物的不同有关。BeO给药组和FCA联合给药组的BE-LT值高于其他实验组。2、Be对雄性和雌性小鼠脾细胞存活率的影响利用小鼠脾细胞，研究了Be对免疫细胞的影响，特别是由于性别差异而引起的细胞存活率的差异。(结果)对照组(Be浓度为0μM)雄性和雌性小鼠的细胞存活率随着孵育时间的延长而降低。雄性小鼠在1、10、100μM时的细胞存活率与对照组相似。在100和1000μM时，与对照组相比有显著降低。雌鼠在1、10μM时细胞存活率变化趋势与对照组相似。在100和1000μM时，与对照组相比，孵育24小时后存活率显著降低。(讨论)BE对雄性小鼠和雌性小鼠的影响差异，雌性小鼠比雄性小鼠更早出现存活率下降。有趣的是，在这项研究中，雌性小鼠的脾细胞存活率下降的时间早于雄性小鼠。我们打算进一步研究这一现象。较少

1. A Study on Sensitization of Guinea Pigs Administered Beryllium (Be) Compound1) The animals were divided into the control group, BeO administration group and BeO+BeSO_4 administration group. A Be specific lymphocyte stimulating test (Be-LT) was performed to study differences in the sensitizing activity.(Results) The BeO administration group showed a significantly high Be-LT value in comparison with the control group and BeO+BeSO_4 administration group.2) The animals were divided into the adjuvant non-administration group (BeSO_4 solution), FCA combined group (BeSO_4+FCA solution) and FIA combined group (BeSO_4+FIA solution). Be-LT was performed to study differences in the sensitizing activity.(Results) The FCA combined group showed a significantly high Be-LT value in comparison with the adjuvant non-administration group.(Discussion) When changes in the Be-LT value were examined in the Be administration group, the Be-LT value in the BeO administration group was high in comparison with … More that in the BeO+BeSO_4 administration group. This suggests that the imnunological effect of intraperitoneal administration of Be compounds works in an accelerating manner in some cases and in suppressing manner in others according to the difference in Be compounds used. Be-LT value in the BeO administration group and FCA combined group was high in comparison with that in the other experimental groups. This suggests that endotracheal administration or intracutaneous administration using FCA is useful.2, Influence of Be on the survival rate in splenic cells of male and female miceUsing mouse splenic cells, we studied the influence of Be on immunocytes, particularly the survival rate due to differences between the sexes.(Results) The cell survival rate in the male and female mice of the control group (Be concentration : 0 μ M) decreased with the lapse of incubation time. The cell survival rate at 1, 10, 100 μM in male mice showed a tendency similar to that in the control. At 100 and 1000 μM, it showed a significant decrease compared with the control. The cell survaval rate at 1 and 10 μM in female mice showed a tendency similar to that in the control. At 100 and 1000 μM, it showed a significant decrease compared with the control from the 24th hour of incubation.(Discussion) Regarding the difference in the influence of Be between male and female mice, of Be between male and female mice, the decline in the survival rate presented itself earlier in female mice than in male mice. Interestingly, the decrease in the splenic cell survival rate in female mice appeared earlier than that in male mice in this study. We intend to study this phenomenon further. Less