Rapid Determination of Phytate and Other Inositol Phosphates

饲料中添加植酸钠可降低大鼠胃肠道钙、镁、铁、锌等矿物质的利用率。然而，几乎60%的植酸盐在大鼠的小肠和盲肠中水解。植酸（IP_6）的水解通常是关于植酸在大鼠粪便中残留量的讨论。肌醇五（IP_5）、四（IP_4）、三（IP_3）、二（IP_2）、单（IP_1）磷酸盐对胃肠道中矿物质利用率的影响尚未讨论，因为没有快速测定这些肌醇磷酸酶的方法。本研究的目的是建立直接进样法测定植酸盐和其他肌醇磷酸盐的方法。使用带UV检测器的HPLC系统的间接光度离子色谱法（matsunaga，et.例如，1988)用于植酸盐和其它肌醇磷酸盐的测定。将植酸钠在6 N-HCl中煮沸2小时，然后用H2O洗涤后蒸发至干。将柠檬酸盐溶于H 0中，用于阴离子交换HPLC系统。肌醇六磷酸可与其它肌醇磷酸酯（IP_5 -IP_1）分离。这些峰被认为是IP_5和IP_4-IP_1（有时IP_5，IP_4，IP_3-IP_1）。由于没有可用的标准，因此没有确定这些标准。利用该方法对不同凝固剂的Momen和Kinugoshi豆腐进行了IP_6分析。木面豆腐样品中肌醇六磷酸能被分离出来，具有较好的分析价值，而粘越豆腐样品中肌醇六磷酸不能被分离出来。

Sodium phytate added into the diets usually reduce the availability of minerals such as Ca, Mg, Fe, Zn in the gastrointestinal tract of rats. However almost 60% of phytate is hydrolysed in the small intestine and the cecum of rats. Hydrolysis of phytate(IP_6)was usually discussed about amount of phytate left in the rat feces. Effects of inositol penta-(IP_5), tetra(IP_4), tri-(IP_3), di-(IP_2), mono-(IP_1)phosphates on mineral availability in the gastrointestinal tracts have not been discussed, because there is no rapid determination of these inositol phosphatase. The objective of this research was to establish a direct injection method, for phytate and other inositol phosphates determination. An indirect photometric ion chromatographic method using a HPLC system with UV detector(matsunaga, et. al., 1988)was carried out for the determination of phytate and other inositol phosphates. Sodium phytate was boiled in 6N-HCl for 2 hours then evaporated to dryness after washing with H 0. Precipitate was disolved in H 0 and was applied to an anion exchange HPLC system. The inositol hexa-phosphate could be separated from other inositol phosphtes(IP_5 -IP_1). Ther were peaks which assumed to be IP_5, and IP_4-IP_1(sometimes IP_5, IP_4, IP_3-IP_1). They were not identified because no standards were available. Momen and Kinugoshi tofe with different kinds of coagurant were analyzed for IP_6 by using this method. Inositol hexaphosphate in samples of Momen tofu was able to separated and has good analytical values, but not in samples of Kinugoshi tofu.