Rapid Determination of Phytate and Other Inositol Phosphates

饲粮中添加植酸钠通常会降低大鼠胃肠道中钙、镁、铁、锌等矿物质的利用率。然而，几乎60%的植酸盐在大鼠的小肠和盲肠中被水解。植酸水解（IP_6）通常是讨论大鼠粪便中残留的植酸的量。肌醇五磷酸（IP_5）、四磷酸（IP_4）、三磷酸（IP_3）、二磷酸（IP_2）、单磷酸（IP_1）磷酸对胃肠道矿物质利用率的影响尚未讨论，因为目前还没有快速测定这些肌醇磷酸酶的方法。本研究的目的是建立一种直接注射法，用于测定植酸和其他磷酸肌醇。采用带紫外检测器的高效液相色谱系统（matsunaga, et al., 1988）间接光度离子色谱法测定植酸盐和其他肌醇磷酸盐。植酸钠在6N-HCl中煮沸2小时，用h0洗涤后蒸发至干燥。沉淀物在h0中溶解，并应用于阴离子交换高效液相色谱系统。六磷酸肌醇可与其他磷酸肌醇（IP_5 -IP_1）分离。存在IP_5、IP_4- ip_1（有时为IP_5、IP_4、IP_3-IP_1）峰。它们没有被识别，因为没有可用的标准。用该方法分析了不同混凝剂的石芋和木芋中IP_6的含量。莫门豆腐样品中的六磷酸肌醇能够分离，具有较好的分析价值，而木谷豆腐样品中没有。

Sodium phytate added into the diets usually reduce the availability of minerals such as Ca, Mg, Fe, Zn in the gastrointestinal tract of rats. However almost 60% of phytate is hydrolysed in the small intestine and the cecum of rats. Hydrolysis of phytate(IP_6)was usually discussed about amount of phytate left in the rat feces. Effects of inositol penta-(IP_5), tetra(IP_4), tri-(IP_3), di-(IP_2), mono-(IP_1)phosphates on mineral availability in the gastrointestinal tracts have not been discussed, because there is no rapid determination of these inositol phosphatase. The objective of this research was to establish a direct injection method, for phytate and other inositol phosphates determination. An indirect photometric ion chromatographic method using a HPLC system with UV detector(matsunaga, et. al., 1988)was carried out for the determination of phytate and other inositol phosphates. Sodium phytate was boiled in 6N-HCl for 2 hours then evaporated to dryness after washing with H 0. Precipitate was disolved in H 0 and was applied to an anion exchange HPLC system. The inositol hexa-phosphate could be separated from other inositol phosphtes(IP_5 -IP_1). Ther were peaks which assumed to be IP_5, and IP_4-IP_1(sometimes IP_5, IP_4, IP_3-IP_1). They were not identified because no standards were available. Momen and Kinugoshi tofe with different kinds of coagurant were analyzed for IP_6 by using this method. Inositol hexaphosphate in samples of Momen tofu was able to separated and has good analytical values, but not in samples of Kinugoshi tofu.