Association of hepatic oval cell and liver diseses : implication to new therapy

肝卵圆细胞与肝脏疾病的关联：对新疗法的意义

• 批准号: 11670504
• 负责人: KAWASAKI Hironaka
• 金额: $ 2.5万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670504/
• 关键词: hepatic oval cell; fulminant hepatitis; HGF; gene therapy; signal transduction

To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt- Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased increased at each time point. The results suggest that in vivo transfer of hepatic oval cells in the Solt-Farber model in rats.Recently, we examined the effect of pAxCAHGF on OC/CDE6 cells, hepatic oval cells. The OC/CDE6 cells, which are infected by pAxCAHGF, grow more rapidly than those by pAXCALacZ.The pathway via which signal of HGF is transmitted is mainly PI3-K, especially Akt. The MAPK is associated with the signal transduction of HGF, but not a main pathway.

为了阐明肝细胞生长因子（HGF）对肝椭圆形细胞增殖的影响，我们将HGF基因转移到溶剂远离大鼠模型的肝脏中。雄性Fisher 344大鼠从尾静脉中感染了携带HGF（PaxCahGF）cDNA的重组腺病毒。 HGF mRNA在4天时显示出峰值，此后减少。在HGF转移的大鼠中，总和增殖的细胞核抗原阳性肝椭圆形细胞显着升高，其中干细胞因子和C-KIT mRNA在每个时间点增加。结果表明，在老鼠的溶液 - 弗尔伯堡模型中，体内转移肝椭圆形细胞。准确地研究了PaxCaHGF对OC/CDE6细胞（肝椭圆形细胞）的影响。被PAXCAHGF感染的OC/CDE6细胞比PaxCalacz生长更快。传播HGF信号的途径主要是PI3-K，尤其是AKT。 MAPK与HGF的信号转导相关，但与主要途径无关。

项目成果

Goshi Shiota et al.: "Invivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oral cells in a 2-acetylaminoflurene/patial hepatectomy modelin rats"FEBS Letters. 470. 325-330 (2000)

Goshi Shiota 等人：“肝细胞生长因子基因的体内转移加速了大鼠 2-乙酰氨基芴/部分肝切除模型中肝口腔细胞的增殖”FEBS 快报。

Goshi Shiota et al.: "In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)

Goshi Shiota 等人：“肝细胞生长因子基因的体内转移加速了大鼠 2-乙酰氨基芴/部分肝切除模型中肝卵圆细胞的增殖。”FEBS 快报。

Goshi Shiota,etal.: "In vivo tsansfer of hepatocyte growth factor gene accelerates prsliferation of hepatic oval cells in a 2-acety lamino fluorme/partial"FEBS Letters. (in press). (2000)

Goshi Shiota等人：“肝细胞生长因子基因的体内转移加速2-乙酰氨基荧光/部分肝卵圆细胞的增殖”FEBS快报。