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ANALYSIS OF HEPATITIS B VIREMIA AMONG HBS ANTIGEN-NEGATIVE PEOPLE (MUTUAL REACTION BETWEEN HBV AND CYTOTOXIC T CELLS)

HBS抗原阴性人群乙型肝炎病毒血症分析（HBV与细胞毒性T细胞的相互反应）

基本信息

批准号：
11670544
负责人：
YOTSUYANAGI Hiroshi
金额：
$ 2.18万
依托单位：
ST.MARIANNA UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670544/
关键词：
hepatitis B virus HBs antigen anti-HBc immune complex hepatocarcinogenesis hepatitis C virus non-B, non-C HCC HBV-DNA S領域 X領域

项目摘要

It has been thought that hepatitis B virus is completely cleared from body of immunocompetent adults after acute hepatitis B.However, recent studies have shown that low levels of viremia continue for long after acute, self-limited hepatitis B.These suggest that a substantial number of people with a previous history of acute, self-limited infection have low levels of HBV in their blood. We have studied the positivity of serum HBV-DNA among 50 blood donors who were negative for HBs antigen (HBsAg) and positive for hepatitis B core antibody (anti-HBc). HBV-DNA was detected in 19 of 50 (38%) of donors. In 8 of the 19 donors have HBV-DNA in the non-immunoglobulin-bound (non-immune-complex) form. Five of the eight donors have HBV-DNA also as immunoglobulin-bound (immune-complex) form. The remaining 11 have HBV-DNA in the immunoglobulin-bound (immune-complex) form alone. Levels of HBV-DNA among HBV-DNA-positive donors were similar to those in the remission phase of acute hepatitis B.Digestion of sera with DNase 1 did not change the levels of HBV-DNA.Amino acid sequences of preS-S region were analyzed in six donors (three with subtype adr and three with subtype adw). Although each donor had different sequence, no common mutation was found. No mutation was found in the major hydrophobic region (MHR). These suggest that some HBsAg-negative, anti-HBc-positive blood donors have infectionsHBV in their sera. We analyzed the effect of such "occult" HBV on hepatocarcinogenesis among HCV-related and non-B, non-C hepatocellular carcinoma (HCC) cases. The effect among HCV-related HCC cases was low. The effect among non-B, non-C HCC cases should be carefully analyzed because genomic structure of such cases was different from that of blood donors.

人们一直认为，急性B型肝炎后，免疫功能正常的成年人体内的B型肝炎病毒会被完全清除。然而，最近的研究表明，急性自限性B型肝炎后，低水平的病毒血症会持续很长时间。这些研究表明，相当数量的人以前有急性自限性感染史，血液中的HBV水平很低。本文对50例HBsAg阴性、抗-HBc阳性的献血员血清HBV-DNA阳性率进行了研究。50例献血者中有19例（38%）检出HBV-DNA。在19名献血者中，有8人的HBV-DNA呈非免疫球蛋白结合（非免疫复合物）形式。8名献血者中有5名也有免疫球蛋白结合（免疫复合物）形式的HBV-DNA。其余11例仅具有免疫球蛋白结合（免疫复合物）形式的HBV DNA。HBV-DNA阳性者血清HBV-DNA水平与急性B型肝炎缓解期相似，DNase 1酶切后血清HBV-DNA水平无明显变化。虽然每个供体的序列不同，但没有发现共同的突变，在主要疏水区（MHR）也没有发现突变。提示部分HBsAg阴性、抗-HBc阳性献血者血清中存在HBV感染。我们分析了这种“隐匿性”HBV在HCV相关和非B、非C型肝细胞癌（HCC）病例中对肝癌发生的影响。在HCV相关的HCC病例中的效果较低。在非B、非C型HCC病例中的作用应仔细分析，因为此类病例的基因组结构与献血员的基因组结构不同。