权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Function of Eosinophils from Eosinophilic pneumonia : Purification and Analysis of Tcell-derived eosinophil chemotactic factor

嗜酸性粒细胞肺炎中嗜酸性粒细胞的功能：T细胞来源的嗜酸性粒细胞趋化因子的纯化和分析

基本信息

批准号：
11670584
负责人：
SAITA Naoki
金额：
$ 2.37万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670584/
关键词：
Eosimophilic patients Apoptosis galectin 9 好酸球性肺炎 IL-5 好酸球遊走因子

项目摘要

Although we couldn't success the finding of structure of eosinophil chemotactic factor derived from T-cell, we could get an interesting result during this time. We assessed the expression of galectin-9 with immunostaining and in situ hybridization both in the lesion of angiolymphoid hyperplasia with eosinophilia, and peripheral blood eosinophils of eosinophilic patients (E-Eos) in comparison with those of normal volunteers (N-Eos). Regulation of expression of galectin-9 on eosinophils and the effect of galectin-9 on apoptosis of eosinophil were also evaluated. Many eosinophils infiltrating the site were positive for galectin-9. Surface and intracellular immunoreactive galectin-9 was more evident in E-Eos than N-Eos. When eosinophils were cultured with IL-5 in vitro, the surface galectin-9 expression of E-Eos was significantly, down-regulated, although that of N-Eos was not affected. Treatment of eosinophils with dexarnethasone or anti-Fas antibody significantly up-regulated the surface galectin-9 expression of E-Eos. In contrast, dexamethasone controversially down-regulated the surface galectin-9 of N-Eos, although anti-Fas antibody failed to affect on the surface galectin-9 expression. We also found that recombinant galectin-9 significantly suppressed apoptosis of E-Eos, whereas it apparently enhanced apoptosis of N-Eos. Furthermore, dexamethasone-induced apoptosis of N-Eos was significantly suppressed by galectin-9, whereas galectin-9 failed to induce significant change in dexamethasoneinduced apoptosis of E-Eos. In contrast, apoptosis induced by anti-Fas antibody in both N-Eos and E-Eos was enhanced by galectin-9. These findings suggested that galectin-9 was produced by eosinophils, and galectin-9 showed heterogeneous effects and kinetics to eosinophils, and this factor might be one of crucial factors in eosinophilic inflammation.

虽然我们没有成功地发现来源于t细胞的嗜酸性趋化因子的结构，但我们在这段时间里得到了一个有趣的结果。我们用免疫染色和原位杂交的方法评估了半凝集素-9在血管淋巴样增生伴嗜酸性粒细胞增多病变中的表达，以及嗜酸性粒细胞增多患者（E-Eos）与正常志愿者（N-Eos）外周血嗜酸性粒细胞的表达。研究了半凝集素-9对嗜酸性粒细胞表达的调控作用以及对嗜酸性粒细胞凋亡的影响。浸润部位的许多嗜酸性粒细胞半凝集素-9阳性。表面和细胞内免疫反应性半凝集素-9在E-Eos比N-Eos更明显。IL-5体外培养嗜酸性粒细胞时，E-Eos表面半凝集素-9的表达明显下调，但N-Eos的表达不受影响。地塞米松或抗fas抗体处理嗜酸性粒细胞可显著上调E-Eos表面半凝集素-9的表达。相反，地塞米松有争议地下调了N-Eos的表面半凝集素-9，尽管抗fas抗体没有影响表面半凝集素-9的表达。我们还发现重组半乳糖凝集素-9显著抑制E-Eos的凋亡，而明显促进N-Eos的凋亡。此外，半凝集素-9能显著抑制地塞米松诱导的N-Eos细胞凋亡，而半凝集素-9不能显著改变地塞米松诱导的E-Eos细胞凋亡。而半凝集素-9可增强抗fas抗体诱导的N-Eos和E-Eos细胞凋亡。这些结果表明，半凝集素-9是由嗜酸性粒细胞产生的，并且对嗜酸性粒细胞具有异质性作用和动力学，该因子可能是嗜酸性粒细胞炎症的关键因素之一。