Myelination in cultured rat peripheral nerve inducted mutant Po c DNA.

培养的大鼠周围神经诱导突变 Po c DNA 中的髓鞘形成。

• 批准号: 11670773
• 负责人: TACHI Nobutada
• 金额: $ 2.3万
• 依托单位: School of Health Sciences, Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670773/
• 关键词: Mutated Po cDNA; constructed adenovirus vector; myelination; 変異Po cDNA; ラツト末梢神経

We identified new mutations of Po genes, including 433A-G, 543G-C, GGCA insertion between 603 to 604, in patients with Charcot-Marie-Tooth disease. We constructed Po cDNA clones containing these mutations by site-directed mutagenesis. These cDNA clones were inserted into expression adenovirus vector by Graham's method. Cultured rat peripheral nerve was peformed using dorsal root ganglions of fetal rat. Now constructed adenovirus vector containg mutated Po cDNA is infected in cultured rat peripheral nerve.

我们在Charcot-Marie-Tooth病患者中发现了Po基因的新突变，包括433 A-G，543 G-C，GGCA插入603至604之间。我们通过定点突变构建了含有这些突变的Po cDNA克隆。用Graham法将这些cDNA克隆插入腺病毒表达载体中。用胎鼠背根神经节培养大鼠周围神经。将构建的含突变Po cDNA的腺病毒载体感染培养的大鼠周围神经。

项目成果

Moreira MC,Tachi N,Koenig M: "Homozygosity mapping of Portuguese and Japanese forms of ataxia-oculomotor apraxia (AOA1) to 9p13 and evidence for genetic heterogeneity"American Journal of Human Genetics. (In press). (2001)

Moreira MC、Tachi N、Koenig M：“葡萄牙和日本形式的共济失调-动眼神经失用症 (AOA1) 到 9p13 的纯合性映射和遗传异质性的证据”美国人类遗传学杂志。

Iinuma K,Minami T,Tachi N et al.: "Long-term effects of zonisamide in the treatment of epilepsy"Journal of Intellectual Disease Research. 42. 68-73 (1998)

Iinuma K、Minami T、Tachi N 等人：“唑尼沙胺治疗癫痫的长期效果”《智力疾病研究杂志》。

Tachi N et al.: "A new mutation of the Po gene in patients with charcot-Marie-Tooth disease type 1 B : screening of the Po gene by heteroduplex analysis."Neurosci Lett. 204. 173-176 (1996)

Tachi N 等人：“1 B 型夏科-玛丽-图思病患者 Po 基因的新突变：通过异源双链分析筛选 Po 基因。”Neurosci Lett。

Lopes J, Ravise N, Tachi N et al: "Fine mapping of de novo CMT1A and HNPP reattangements"Human Molecular Genetics. 7. 141-148 (1998)

Lopes J、Ravise N、Tachi N 等人：“从头 CMT1A 和 HNPP 重新连接的精细定位”人类分子遗传学。

Suzuki Y,Wada T,Tachi N et al.: "Phenotypic variability in a family with a mitochondrial DNA"Pediatric Neurology. 19. 283-286 (1998)

Suzuki Y、Wada T、Tachi N 等人：“具有线粒体 DNA 的家族的表型变异”小儿神经病学。