Cloning and characterization of desmosome and keratin binding proteins.
桥粒和角蛋白结合蛋白的克隆和表征。
基本信息
- 批准号:11670826
- 负责人:
- 金额:$ 2.5万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We have studies the function of the cytoarchitecture of the human keratinocytes in the aspect of the binding proteins to the keratin intermediate filament sand desmosomal apparatus. To identify the novel function of these well characterized structural proteins, we used the yeast two hybrid system to clone the keratin and desmosome binding proteins.Desmocollins constitute the extracellular part of desmosomal complexes with another desmosomal cadherin, desmogleins. These two desmosomal cadherins consist of three distinct isoforms, Dsc 1-3 and Dsg 1-3, and each desmocollin isoform has two splicing forms, the longer Dsc1a-3a and the shorter Dsc1b-3b. However, the function of the intracellular domain of shorter Dsc is yet unknown. Again, we used the yeast two-hybrid system to find out proteins binding to the carboxyl end of Dsc 2b from human skin cDNA library. Rack1, receptor for activated C kinase, was isolated as an interacting protein with the intracellular region of all Dsc b forms. Thu … More s the cytoplasmic tail of desmocollins was suggested to act as an anchoring site of PKC and to have a function modifying the cell adhesion through the phosphorylation of desmosomal proteins.We next attempted to clone and characterize a novel protein, which interact with KIF at the cytoplasmic region of the human skin by yeast two-hybrid system. Employing the head domain of type I keratin K14, K16 as a bait, we screened the human skin cDNA and could obtain a candidate clone. The cloned gene consists of approx. 4.0kbp and has relatively large 1.0kbp 5' untranslating region. The predicted gene product is 105 kD protein encoded by 970 amino acids with no significant homology with known proteins. This protein contains several nuclear localization signals as well as one putative transmembrane segment in its carboxyl half. The isolated clone showed the obvious binding with type I keratin, K14 and K18, but no interaction with type II keratin, K6 and K8 at least in the yeast system. When the cloned gene was fused with green fluorescence protein (GFP) and used for the forced expression into cultured keratinocytes, the GFP signals are observed predominantly along with the nuclear membrane. These nuclear signals were overlapped with keratin localization around the nuclei in the confocal-microscopic analysis. These data suggest that this newly identified clone encodes a cross linking protein, which binds the KIF to the nuclear membrane. Less
我们从角蛋白中间丝和桥粒器的结合蛋白方面研究了人角质形成细胞的细胞构筑功能。为了鉴定这些结构蛋白的新功能,我们利用酵母双杂交系统克隆了角蛋白和桥粒结合蛋白,桥粒结合蛋白与桥粒钙粘蛋白桥粒糖蛋白构成桥粒复合物的胞外部分。这两种桥粒钙粘蛋白由三种不同的同种型Dsc 1-3和Dsg 1-3组成,并且每种桥粒胶原同种型具有两种剪接形式,较长的Dsc 1a-3a和较短的Dsc 1b-3b。然而,较短的Dsc的胞内结构域的功能还不清楚。利用酵母双杂交技术从人皮肤cDNA文库中筛选出与Dsc 2b羧基端结合的蛋白。Rack 1,活化C激酶的受体,被分离为与所有Dsc B形式的细胞内区域相互作用的蛋白。Thu ...更多信息 结果表明,桥粒蛋白的胞质尾区可能是PKC的锚定位点,并通过桥粒蛋白的磷酸化修饰细胞的粘附,因此,我们尝试利用酵母双杂交系统,在人皮肤胞质区克隆一个与KIF相互作用的新蛋白。以Ⅰ型角蛋白K14、K16的头部结构域为诱饵,筛选人皮肤cDNA,获得一个候选克隆。克隆的基因由大约。4.0 kbp,5'端非翻译区较大,约为1.0kbp。预测的基因产物是由970个氨基酸编码的105 kD蛋白,与已知蛋白没有明显的同源性。该蛋白含有几个核定位信号,以及一个假定的跨膜片段在其羧基的一半。该克隆与Ⅰ型角蛋白、K14和K18有明显的结合,而与Ⅱ型角蛋白、K6和K8无相互作用。当克隆的基因与绿色荧光蛋白(GFP)融合并用于强制表达到培养的角质形成细胞中时,主要观察到GFP信号沿着核膜。在共聚焦显微镜分析中,这些核信号与核周围的角蛋白定位重叠。这些数据表明,这个新鉴定的克隆编码一种交联蛋白,该蛋白将KIF结合到核膜上。少
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kenzo Takahashi et al: "Introducing a Null Mutation in the Mouse K6α and K6β Genes Reveals Theiressential Structural Role in Oral Mucosa Epithelia"J.Cell Biol.. 150(4). 921-928 (2000)
Kenzo Takahashi 等人:“在小鼠 K6α 和 K6β 基因中引入无效突变揭示了它们在口腔粘膜上皮中的基本结构作用”J.Cell Biol.. 150(4) (2000)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
R Inada et al.: "Facilitated Wound Healing by Activation of the Transglutaminase 1 Gene."Am J Pathol.. 157(6). 1875-1882 (2000)
R Inada 等人:“通过转谷氨酰胺酶 1 基因的激活促进伤口愈合”。Am J Pathol.. 157(6)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kenzo Takahashi et al: "Facilitated Wound Healing by Activation of the Transglutaminase 1 Gene."Am J Pathol. 157(6). 1875-1882 (2000)
Kenzo Takahashi 等人:“通过激活转谷氨酰胺酶 1 基因促进伤口愈合。”Am J Pathol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K Takahashi et al.: "Using transgenic models to study the pathogenesis of keratin-based inherited skin diseases."J.Dermatol.Sci.. 21(2). 73-95 (1999)
K Takahashi 等人:“使用转基因模型研究基于角蛋白的遗传性皮肤病的发病机制。”J.Dermatol.Sci. 21(2)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kenzo Takahashi et al: "Using transgenic models to study the pathogenesis of keratin-based inherited skin diseases."J.Dermatol.Sci.. 21(2),73-95 1999. 73-95 (1999)
Kenzo Takahashi 等人:“使用转基因模型研究基于角蛋白的遗传性皮肤病的发病机制。”J.Dermatol.Sci.. 21(2),73-95 1999. 73-95 (1999)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
TAKAHASHI Kenzo其他文献
A Case of Hallopeau Type Pemphigus Vegetans Successfully Treated with High-dose Intravenous Immunoglobulin(IVIG)Therapy
大剂量静脉注射免疫球蛋白(IVIG)成功治疗Hallopeau型素食性天疱疮一例
- DOI:
10.2336/nishinihonhifu.79.552 - 发表时间:
2017 - 期刊:
- 影响因子:0
- 作者:
YAGI Nobutake;YAMAGUCHII Sayaka;TAKAHASHI Kenzo - 通讯作者:
TAKAHASHI Kenzo
Malignant Melanoma in a Pregnant Woman
孕妇的恶性黑色素瘤
- DOI:
10.2336/nishinihonhifu.82.94 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
SAKUGAWA Hiroyuki;YAMAGUCHI Sayaka;YAMASHIRO Atsushi;KARIYA Yoshiyuki;ARAKAKI Osao;YAMAMOTO Yu-ichi;TAKAHASHI Kenzo - 通讯作者:
TAKAHASHI Kenzo
Two Cases of Squamous Cell Carcinoma Arising on Refractory Skin Ulcers in Patients with a History of Hansen's Disease
有汉森病史患者难治性皮肤溃疡所致鳞状细胞癌二例
- DOI:
10.2336/nishinihonhifu.81.115 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
FUKAI Kyoko;KOMATSU Kotaro;MATSUO Yuji;HAYASHI Kentaro;KARIYA Yoshiyuki;MIYAGI Takuya;YAMAGUCHI Takuya;TERUYA Misao;TAKAHASHI Kenzo - 通讯作者:
TAKAHASHI Kenzo
Successful Treatment with Methotrexate for Eosinophilic Fasciitis
甲氨蝶呤成功治疗嗜酸性筋膜炎
- DOI:
10.2336/nishinihonhifu.81.289 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
HORIGUCHI Ayumi;MIYAGI Takuya;YAMAGUCHI Sayaka;TAKAHASHI Kenzo - 通讯作者:
TAKAHASHI Kenzo
A Case of Iatrogenic Kaposi's Sarcoma Relapsed with Worsening of the General Condition with Pneumonia and Urinary Tract Infection
医源性卡波西病一例
- DOI:
10.2336/nishinihonhifu.81.491 - 发表时间:
2019 - 期刊:
- 影响因子:0
- 作者:
HORIGUCHI Ayumi;YAMAGUCHI Sayaka;AWAZAWA Ryoko;TAKAHASHI Kenzo - 通讯作者:
TAKAHASHI Kenzo
TAKAHASHI Kenzo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('TAKAHASHI Kenzo', 18)}}的其他基金
The exhaustive search of foreign DNAs as the pathogenic factor of angiosarcoma.
外源 DNA 作为血管肉瘤致病因子的详尽研究。
- 批准号:
24591631 - 财政年份:2012
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The efficacy of cannabinoids to the various keratoderma.
大麻素对各种角化病的功效。
- 批准号:
21591462 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The therapeutic effects of TRPV receptor agonists for Darier disease
TRPV受体激动剂对达里尔病的治疗作用
- 批准号:
19591302 - 财政年份:2007
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
New Hearing Aid System Relieving Old Age People from Hearing Loss toward the Future Ubiquitous Society
新型助听器系统缓解老年人听力损失,迈向未来无所不在的社会
- 批准号:
18560372 - 财政年份:2006
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Characterization of re-programming factors induces the body site-specific differentiation of epidermal keratinocytes.
重编程因子的表征诱导表皮角质形成细胞的身体部位特异性分化。
- 批准号:
15390342 - 财政年份:2003
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Cloning of novel binding protein, which links Keratin filaments to the nuclear envelops.
克隆新型结合蛋白,将角蛋白丝与核膜连接起来。
- 批准号:
13670883 - 财政年份:2001
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Fate of migrating epidermal cells during the wound healing using the injured-epithelia specific gene recombination.
使用受伤上皮特异性基因重组在伤口愈合过程中迁移表皮细胞的命运。
- 批准号:
10557080 - 财政年份:1998
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
相似海外基金
Towards the use of Barcode Fusion Genetics Yeast-Two Hybrid and Edgotyping to Characterize Homeostatic and Disease Interactomes
致力于使用条形码融合遗传学酵母二杂交和 Edgotyping 来表征稳态和疾病相互作用组
- 批准号:
537335-2018 - 财政年份:2018
- 资助金额:
$ 2.5万 - 项目类别:
Alexander Graham Bell Canada Graduate Scholarships - Master's
Development of the Mammalian Membrane Two-Hybrid (MaMTH) technology, a novel protein interaction and drug discovery tool
开发哺乳动物膜双杂交 (MaMTH) 技术,一种新型蛋白质相互作用和药物发现工具
- 批准号:
268522 - 财政年份:2012
- 资助金额:
$ 2.5万 - 项目类别:
Operating Grants
SBIR Phase I: A Deep Sequencing-Based Platform for Yeast Two-Hybrid Screening
SBIR 第一阶段:基于深度测序的酵母双杂交筛选平台
- 批准号:
1215608 - 财政年份:2012
- 资助金额:
$ 2.5万 - 项目类别:
Standard Grant
Characterizing the Sho1p interactome in Saccharomyces cerevisiae using the membrane Yeast Two-Hybrid (MYTH) system
使用膜酵母双杂交 (MYTH) 系统表征酿酒酵母中的 Sho1p 相互作用组
- 批准号:
372393-2012 - 财政年份:2012
- 资助金额:
$ 2.5万 - 项目类别:
Discovery Grants Program - Individual
Indentification of Rubisco interactants in Gonyaulax by yeast two hybrid assays
通过酵母两种杂交测定鉴定膝沟藻中的 Rubisco 相互作用物
- 批准号:
415415-2011 - 财政年份:2011
- 资助金额:
$ 2.5万 - 项目类别:
University Undergraduate Student Research Awards
SYSTEMATIC TWO-HYBRID AND COMPARATIVE PROTEOMIC ANALYSES
系统的双杂交和比较蛋白质组分析
- 批准号:
8365862 - 财政年份:2011
- 资助金额:
$ 2.5万 - 项目类别:
Analysis of potential interactors of atPUB18 and AtPUB19 Using yeast-two hybrid experiment
利用酵母-二杂交实验分析 atPUB18 和 AtPUB19 的潜在相互作用因子
- 批准号:
363006-2009 - 财政年份:2010
- 资助金额:
$ 2.5万 - 项目类别:
Postgraduate Scholarships - Master's
Two-hybrid screen for cellular fusion factors
细胞融合因子的双杂交筛选
- 批准号:
383598-2009 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
University Undergraduate Student Research Awards
Development of the mammalian membrane two-hybrid technology
哺乳动物膜二杂交技术的开发
- 批准号:
183284 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
Operating Grants
Development of the mammalian membrane two-hybrid technology.
哺乳动物膜二杂交技术的开发。
- 批准号:
182461 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
Operating Grants