Characterization of re-programming factors induces the body site-specific differentiation of epidermal keratinocytes.

重编程因子的表征诱导表皮角质形成细胞的身体部位特异性分化。

• 批准号: 15390342
• 负责人: TAKAHASHI Kenzo
• 金额: $ 9.22万
• 依托单位: Kyoto University Graduate School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390342/
• 关键词: re-programing; cDNA microarray; subtraction; fibroblast; fibronectin; EGF motif; lumican; decorin; cDNAマイクロアレー; 表皮角化細胞; 再プログラム化; マイクロアレー; 掌蹠皮膚; 粘膜口唇部; 体幹部皮膚

BackgroundHuman skin shows various morphological characteristics, depending on the body site. As these distinct phenotypes have been explained on the basis of the variance in epidermal keratinocytes and the presence of skin appendages, the spatial distinction of the dermal components has not been fully elucidated.ObjectivesTo identify and characterize the profiles of mRNAs that are abundantly or specifically expressed by fibroblasts derived from trunk skin, but not from palmoplantar skin or oral mucosa.MethodsIn order to identify the distinct mRNA expression by trunk skin fibroblasts, a subtraction cDNA screening was first performed, followed by northern blotting, western blotting and immuno-histochemistry. Finally, whole mount in situ hybridization (WISH) was performed to examine the differences in the expression of corresponding gene during the developmental stage of mouse embryos.ResultsWe identified three cDNA clones encoding fibronectin (FN), pregnancy-specific β1-glycoprotein 5 and β-actin respectively, whose mRNAs were abundantly or specifically expressed by trunk skin fibroblasts. FN and some integrins were further confirmed to be expressed more selectively in trunk skin fibroblasts, both in terms of the RNA and the protein levels, as compared with the fibroblasts derived from the plamoplantar skin and oral mucosa. WISH demonstrated that FN was localized around the hair follicles of mouse embryos.ConclusionsFN, one of most potent extracellular matrix molecules, was demonstrated to be spatially transcribed depending on the body sites. The distinct expression of FN was suggestive of the essential commitment in the process of cutaneous development and morphogenesis of appendages.The paucity of FN in palmoplantar skin and oral mucosa might explain the characteristics of these skin phenotypes.

人体皮肤根据身体部位的不同表现出不同的形态特征。由于这些不同的表型是根据表皮角质形成细胞的差异和皮肤附属物的存在来解释的，因此真皮成分的空间差异尚未完全阐明。目的鉴定来自躯干皮肤而非掌跖皮肤或口腔粘膜的成纤维细胞大量或特异性表达的mrna的特征。方法先进行减法cDNA筛选，然后进行northern blotting、western blotting和免疫组化，以鉴定躯干皮肤成纤维细胞mRNA的表达。最后，采用全载原位杂交（WISH）技术检测相应基因在小鼠胚胎发育阶段的表达差异。结果鉴定出3个分别编码纤维连接蛋白（FN）、妊娠特异性β1-糖蛋白5和β-肌动蛋白的cDNA克隆，它们的mrna在干皮肤成纤维细胞中大量或特异性表达。与足底和口腔粘膜的成纤维细胞相比，FN和一些整合素在躯干皮肤成纤维细胞中的RNA和蛋白质水平均有更高的选择性表达。WISH显示FN定位于小鼠胚胎毛囊周围。结论sfn是最有效的细胞外基质分子之一，它的空间转录依赖于身体部位。FN的独特表达暗示了其在皮肤发育和附属物形态发生过程中的重要作用。掌跖皮肤和口腔黏膜中FN的缺乏可能解释了这些皮肤表型的特征。

项目成果

Spatial expressions of fibronectin and integrins by human and rodent dermal fibroblasts

• DOI: 10.1111/j.1365-2133.2006.07399.x
• 发表时间: 2006-09-01
• 期刊: BRITISH JOURNAL OF DERMATOLOGY
• 影响因子: 10.3
• 作者: Yasuda, M.;Miyachi, Y.;Takahashi, K.
• 通讯作者: Takahashi, K.

Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal cord.

骨髓基质细胞增强共培养神经球细胞的分化并促进受损脊髓的再生。

• 发表时间: 2003
• 期刊: J Neurosci Res. 72(3)
• 作者: Wu S;Suzuki Y;Ejiri Y;Noda T;BaiI H;Kitada M;Kataoka K;Ohta M;Chou H;Ide C.
• 通讯作者: Ide C.

表皮細胞生物学の最新の知見

表皮细胞生物学的最新发现

• 发表时间: 2005
• 期刊: 日本皮膚科学会雑誌 115(13)
• 作者: Tanioka M;Takahashi K;Kawabata T;Kosugi S.Murakami K;Miyachi Y;Nishigori C;Iizuka T.;高橋 健造
• 通讯作者: 高橋 健造

Alginate enhances elongation of early regenerating axons in spinal cord of young rats

• DOI: 10.1089/107632704323061852
• 发表时间: 2004-03-01
• 期刊: TISSUE ENGINEERING
• 作者: Kataoka, K;Suzuki, Y;Ide, C
• 通讯作者: Ide, C

Specific induction of neuronal cells from bone marrow stromal cells and application for autologous transplantation.

• DOI: 10.1172/jci20935
• 发表时间: 2004-06
• 期刊: The Journal of clinical investigation
• 作者: M. Dezawa;H. Kanno;M. Hoshino;Hirotomi Cho;N. Matsumoto;Yutaka Itokazu;Nobuyoshi Tajima;Hitoshi Yamada-Hi