Oxygenation status of head and neck tumor evaluated with near infrared reflection spectroscopy

用近红外反射光谱评估头颈部肿瘤的氧合状态

• 批准号: 11670859
• 负责人: MATSUTANI Hideya
• 金额: $ 1.41万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670859/
• 关键词: Near Infrared Reflection; Oxygenation Status; Head and neck Tumor; Normal Tissue; Hypoxia; Radioresistance

Using a non-invasive system with near infrared reflection spectroscopy (NIR), we studied the oxygenation status of primary and metastatic lesions of the head and neck tumors. In the fiscal year of 1999, we studied the status of the oxyhemoglobin (oxyHgb) and in the fiscal year of 2000, we analyzed the status of the cytochrome aa3 (Cyt).In the head and neck region, the mucosa were found to be higher oxyHgb values than the skin. Especially, buccal mucosa showed the highest. There were no correlations between oxyHgb and PaO_2 and between oxyHgb and peripheral blood Hgb. Tissue oxyHgb levels measured using NIR were not affected by oxygenation status of the blood. OxyHgb values in all metastaic lymph nodes were significantly low compared with normal tissues suggesting hypoxia. Primary lesions showed either normal or low oxyHgb suggesting normoxic and hypoxic status of the tumor.Cyt levels in head and neck tumors were significantly low compared with normal tissues. There were no correlations between Cyt and PaO_2 and between Cyt and peripheral blood Hgb. Cyt values in the normal tissues and tumors were not affected by the status of blood. Combined with the data obtained from oxyHgb and Cyt, it suggested that anaerobic and aerobic metabolism was the main source for tumors and for the normal tissues, respectively.For the terms of 1999 and 2000, we studied oxygenation status for normal tissue and tumors in humans using NIR.It was important to estimate the tumor hypoxia because of a factor for radioresistance. We conclude NIR is a useful method for radiation therapy.

应用无创近红外反射光谱(NIR)系统，研究头颈部肿瘤原发灶和转移灶的氧合状态。在1999财政年度，我们研究了氧合Hgb的状况，在2000财政年度，我们分析了细胞色素Aa3(Cyt)的状况。在头颈部，粘膜的氧合Hgb值高于皮肤。其中以颊粘膜最高。氧合Hgb与PaO2、外周血Hgb均无相关性。用近红外光谱测量的组织氧合Hgb水平不受血液氧合状态的影响。与正常组织相比，所有转移淋巴结的OxyHgb值均显著降低，提示有缺氧现象。原发灶显示正常或低氧Hgb，提示肿瘤处于正常和低氧状态。与正常组织相比，头颈部肿瘤的细胞水平显著降低。Cyt与PaO2及外周血HGB均无相关性。正常组织和肿瘤组织中的细胞色素T值不受血液状态的影响。结合氧合Hgb和细胞色素T的数据，表明无氧代谢和有氧代谢分别是肿瘤和正常组织的主要来源。1999年和2000年，我们利用近红外光谱研究了人体正常组织和肿瘤的氧合状态。由于辐射抵抗的因素，估计肿瘤的缺氧具有重要意义。我们认为，近红外是一种有效的放射治疗方法。

项目成果

Y.Mariya: "Oxygenation status and tumor response during fractionated irradiation in two murine tumor cell lines of same origin but different intrinsic radiosensitivities"Radiation Medicine. 17・2. 175-179 (1999)

Y.Mariya：“两种来源相同但内在放射敏感性不同的小鼠肿瘤细胞系中的氧合状态和肿瘤反应”17・2（1999）。

Yasushi Mariya: "Oxygenation status and tumor response during fractionated irradiation in two murine tumor cell lines of same origin but different intrinsic radiosensitivities."Radiation Medicine. 17-2. 175-179 (1999)

Yasushi Mariya：“两种来源相同但内在放射敏感性不同的鼠肿瘤细胞系在分次照射过程中的氧合状态和肿瘤反应。”放射医学。

Yoshinao Abe: "Clinical and fundamental researches of radiotherapy."Hirosaki Medical Journal. 51(Suppl). S203-207 (1999)

阿部义直：“放射治疗的临床和基础研究。”弘前医学杂志。

Hidehiro Kondo: "Comet assay as a predictive assay for radiosensitivity of two human brain tumor cell lines."Hirosaki Medical Journal. (in press).

Hidehiro Kondo：“彗星测定作为两种人脑肿瘤细胞系放射敏感性的预测测定。”弘前医学杂志。

Yoshinao Abe: "Clinical and fundamental researches of radiotherapy"Hirosaki Medical Journal. 51(Suppl). S203-207 (1999)

阿部义直：《放射治疗的临床与基础研究》弘前医学杂志。