PREDICTIVE SCREENING ASSAY FOR RADIATION INDUCED DAMAGES CAUSED BY CLINICAL RADIOTHERAPY

临床放射治疗引起的辐射损伤的预测筛查分析

• 批准号: 11670913
• 负责人: SHIGEMATSU Naoyuki
• 金额: $ 2.24万
• 依托单位: KEIO University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670913/
• 关键词: Radiotherapy; Chromosomal aberration; Total body irradiation; Late-radiation toxicity; 放射線照射; 細胞周期; アポトーシス; 遺伝子突然変異

We have studied the prediction assay of radiation induced secondary malignancy by using in vitro human cell lines. Previously, we reported the comparison of the mutation induction (hprt locus) by heavy ion beams and X-rays when cells were irradiated. In this report, we described the chromosomal aberration (dicentric and fragment) of human lymphocyte taken from patients before and after total body irradiation (TBI). Bloods were drawn several times during the TBI and lymphocytes were isolated, and then observed by Giemsa-staining. We also took lymphocytes from patients before TBI, and then they were irradiated under the same schedule of TBI, and evaluated in * same assay. The dicentric frequencies and fragment numbers were well related with radiation dose both in vivo (TBI) and in vitro studies (figure1.2). Our experimental results indicated that this assay system would be applicable to lymphocytes from patients who receive irradiation therapy.

我们利用体外人类细胞系研究了辐射诱发继发性恶性肿瘤的预测分析。之前，我们报道了重离子束和X射线照射细胞时的突变诱导（hprt位点）的比较。在本报告中，我们描述了全身照射（TBI）前后从患者身上采集的人类淋巴细胞的染色体畸变（双着丝粒和片段）。 TBI期间多次抽血并分离淋巴细胞，然后通过吉姆萨染色观察。我们还在 TBI 前采集了患者的淋巴细胞，然后按照与 TBI 相同的时间表对它们进行照射，并在相同的测定中进行评估。双着丝粒频率和片段数与体内（TBI）和体外研究中的辐射剂量密切相关（图1.2）。我们的实验结果表明，该测定系统适用于接受放射治疗的患者的淋巴细胞。

项目成果

Naoyuki Shigematsu et.al.: "Cell killing and mutation by heavy ion beams"Int.J.Molecul.Med.. (printing). (2001)

Naoyuki Shigematsu 等人：“重离子束杀死细胞和突变”Int.J.Molecul.Med..（印刷版）。