PREDICTIVE SCREENING ASSAY FOR RADIATION INDUCED DAMAGES CAUSED BY CLINICAL RADIOTHERAPY

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We have studied the prediction assay of radiation induced secondary malignancy by using in vitro human cell lines. Previously, we reported the comparison of the mutation induction (hprt locus) by heavy ion beams and X-rays when cells were irradiated. In this report, we described the chromosomal aberration (dicentric and fragment) of human lymphocyte taken from patients before and after total body irradiation (TBI). Bloods were drawn several times during the TBI and lymphocytes were isolated, and then observed by Giemsa-staining. We also took lymphocytes from patients before TBI, and then they were irradiated under the same schedule of TBI, and evaluated in * same assay. The dicentric frequencies and fragment numbers were well related with radiation dose both in vivo (TBI) and in vitro studies (figure1.2). Our experimental results indicated that this assay system would be applicable to lymphocytes from patients who receive irradiation therapy.