Study on mechanisms for inducing periventricular leucomalacia prior to delivery usig extrauterine incubation system of a goat fetus.

山羊胎儿宫外孵化系统产前诱发脑室周围白质软化的机制研究。

• 批准号: 11671075
• 负责人: YOSHIDA Koyo
• 金额: $ 2.3万
• 依托单位: Juntendo university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671075/
• 关键词: experimental perinatology; artificial womb; periventriclar leucoma ; acia; cerebral palsy; experimental model; fetus; ECMO; 脳性マヒ; variable deceleration; behavioral state

To investigate the mechanisms for inducing periventricular leucomalacia (PVL) prior to delivery, we attempted to use the extrauterine incubation system on a goat fetus. It was hypothesized that consecutive ischemia and reperfusion to the brain of premature fetuses caused by transient umbilical venous compression could be a causative agent in the induction of PVL. Eleven goat fetuses of approximately 125 days gestational age (term 145 days) were incubated using extrauterine incubation system with arterio-venous extracorporeal membrane oxygenation (A-V ECMO). Acatheter connected to the umbilical vein was occluded completely (total occlusion in 5 cases) or partially (75% occlusion in 6 cases) for 15 seconds and then relieved from occlusion. Total volume of blood stored in a syringe during the occlusion was transfused over the next 45 seconds through the catheter. This procedure was repeated consecutively 6 times at intervals of 5 minutes. After the procedure, extrauterine incubation was c … More ontinued for up to 4 days. The goat fetus was then sacrificed, and the brain tissue was examined histopathologicaUy for the presence PVL lesions. During occlusion of the umbilical vein catheter, there was a significant decrease in fetal heart rate, fetalblood pressure and flow volume of the carotid artery as well as the amplitude of electrocorticogram. However, each of these recovered soon after relief from occlusion. There were no apparent differences between pre- and post- procedure blood gas examinations. The lesion of coagulation necrosis, which was recognized as the primary lesion of PVL, could be observed in the deep white matter surrounding the lateral ventricle in seven out of eleven goat fetuses. These results suggest that consecutive umbilical venous compression causing ischemia and reperfusion to the brain of premature fetuses may play an important role in the induction of PVL. As well, the extrauterine incubation system could provide an experimental animal model to investigate fetal pathology. Less

为了探讨分娩前诱发脑室周围白质软化(PVL)的机制，我们尝试在山羊胎儿上使用宫外孵化系统。推测一过性脐静脉压迫引起的早产儿连续脑缺血再灌流可能是诱发PVL的原因之一。11只胎龄约125天(足145天)的山羊胚胎在动静脉体外膜氧合(A-V ECMO)宫外孵化系统中孵化。连接脐静脉的导管完全闭塞(5例完全闭塞)或部分闭塞(6例75%闭塞)15s后解除闭塞。在接下来的45秒内，通过导管将堵塞期间储存在注射器中的血液总量输入。该过程以5分钟为间隔连续重复6次。术后行c…宫外孵化。更多的持续时间长达4天。然后处死山羊胎儿，并对脑组织进行组织病理学检查，以确定是否存在PVL病变。阻断脐静脉导管时，胎儿心率、胎儿血压、颈动脉血流量及皮层脑电波幅均显著降低。然而，在咬合解除后不久，这些都恢复了。术前、术后血气检查无明显差异。11只山羊胎儿中有7只在侧脑室周围深部白质可见凝固性坏死病变，被认为是PVL的原发病变。这些结果提示，脐静脉持续压迫导致早产儿脑缺血再灌流可能在PVL的诱发中起重要作用。此外，宫外孵化系统可以为研究胎儿病理提供一个实验动物模型。较少

项目成果

吉田幸洋: "妊娠の生物学(分担) ヤギ胎仔を用いた子宮外保育実験"永井書店. 323-328 (2001)

吉田幸弘：《妊娠生物学（共享）使用山羊胎儿的宫外孵化实验》永井书店 323-328（2001）。

湯原 千治: "子宮外ヤギ胎仔保育システムを用いた脳室周囲白質軟化症(PVL)の発生に関する研究"日本新生児学会誌. 37・1. 36-41 (2001)

Chiji Yuhara：“使用宫外山羊胎儿护理系统对脑室周围白质软化症（PVL）发生的研究”日本新生儿学会杂志37・1（2001）。

米本 寿志: "臍帯動静脈A-V ECMOによる子宮外保育中のヤギ胎仔における心拍変動のスペクトル解析"日本産科婦人科学会雑誌. 53・12. 1819-1825 (2001)

Hisashi Yonemoto：“使用脐带动静脉 A-V ECMO 对山羊胎儿在子宫外孵化期间的心率波动进行频谱分析”​​日本妇产科学会杂志 53・12（2001 年）。

吉田 幸洋: "人工子宮の可能性"周産期医学. 31・1. 79-83 (2001)

吉田幸弘：“人工子宫的可能性”《围产期医学》31・1（2001）。

西岡 暢子: "人工子宮の可能性"産婦人科の世界. 53・8. 129-133 (2001)

西冈信子：“人工子宫的可能性”《妇产科世界》53・8（2001）。