Interactions of Bio-Polymers and Lipids in Layered Structures

层状结构中生物聚合物和脂质的相互作用

• 批准号: 5297312
• 负责人: Professor Dr. Helmuth Möhwald (†)
• 金额: --
• 依托单位: Max-Planck-Institut für Kolloid- und Grenzflächenforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5297312?language=en
• 关键词: Interactions; Bio; Polymers; Lipids; Layered

The interaction of phospholipids with polyelectrolytes and glycosidic polymers, both of biological origin, shall be investigated for layered systems. For amphiphile monolayers structural studies with concern the lipid arrangement in monolayers studied by X-ray diffraction and FTIR spectroscopy. The polymer arrangement shall be investigated by ellipsometry, X-ray and Neutron reflectivity. The coupling shall be studied by FTIR and NMR spectroscopy with vesicles and coated colloids. Lateral diffusion of lipids will be investigated by fluorescence recovery after photobleaching. NMR techniques will also be employed to investigate the swelling of films and the fraction of water in the film as well a between film and lipid head groups. Of special interest will be the relation between coupling and dynamics, so1/gel transitions of polyelectrolytes and glycosidic polymers and their importance for modulation or ligand binding. Altogether the project aims at understanding the relation between structure and dynamics in coupled lipid/polymer systems and its relevance for biophysics and -chemistry.

对于层状体系，应研究磷脂与生物来源的聚电解质和糖苷聚合物的相互作用。对于两亲单分子膜的结构研究，用X射线衍射和傅里叶变换红外光谱研究了单分子膜中的脂类排列。聚合物的排列应通过椭偏仪、X射线和中子反射率进行研究。用FTIR和核磁共振波谱研究囊泡和包覆胶体的偶联作用。通过光漂白后的荧光恢复来研究脂类的横向扩散。核磁共振技术也将被用来研究薄膜的溶胀和薄膜中的水的比例，以及薄膜和脂头基团之间的关系。特别感兴趣的是耦合和动力学之间的关系，聚电解质和糖苷聚合物的SO1/凝胶转变，以及它们对调节或配体结合的重要性。总而言之，该项目旨在了解脂类/聚合物耦合系统中结构和动力学之间的关系及其与生物物理和化学的相关性。