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Induction of stress protein (HSP70) and control mechanism of fibroblast activation

应激蛋白（HSP70）的诱导和成纤维细胞活化的控制机制

基本信息

批准号：
11671191
负责人：
KIYAMA Teruo
金额：
$ 2.18万
依托单位：
Nippon Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671191/
关键词：
wound healing stress protein heat shock protein 70 (HSP70)collagen colon anastomotic healing heat shock protein HSP70 高圧酸素療法大腸吻合熱ショック蛋白 heat shock protein 70 線維芽細胞皮膚大腸消化管吻合栄養

项目摘要

Fibroblast is one of the main cells of wound healing. In this project we investigated the expression of heat shock protein 70, the proliferation, and total protein synthesis of fibroblast. 1) HSP70 was expressed 1 hour after heat shock and the peak of expression of HSP70 was 8 hour after heat shock. 2) the proliferation of fibroblast was lower in heat shock group than in the control (p<0.01). 3) Collagen synthesis was not different between the heat shock and the control. Total protein synthesis was increased in the heat shock (p<0.01). Heat shock (43℃, 1 hour) induced the expression of heat shock protein 70 in fibroblast. Heat shock decreased the collagen synthesis but increased the total protein synthesis in fibroblast.The expression of HSP was observed during wound healing process but it is difficult to induce HSP artificially. Hyperbaric oxygen increased the expression of HSP slightly. In the patients with anastomotic failure, the coagulation factor XIII administration enhanced the expression of growth factors, such as TGF, in the serum. Coagulation factor XIII administration also increased TGF expression in vitro. Immediate postoperative nutritional support increased body weight and colon anastomotic bursting pressure in rats. The single layer results in less damage to the tissue edges and preserves collagen network. The mattress sutures increase inflammatory response and the ischemia of the inverted tissue. The intention of anastomotic healing may depend on the suture method.

成纤维细胞是创面愈合的主要细胞之一。本课题研究了热休克蛋白70的表达、成纤维细胞的增殖和总蛋白的合成。1) HSP70在热休克后1 h表达，在热休克后8 h达到表达高峰。2)热休克组成纤维细胞增殖明显低于对照组（p<0.01）。3)热休克组与对照组胶原合成无显著差异。热休克时总蛋白合成增加（p<0.01）。热休克（43℃，1小时）诱导成纤维细胞热休克蛋白70的表达。热休克使成纤维细胞胶原合成减少，总蛋白合成增加。在创面愈合过程中观察到热休克蛋白的表达，但人工诱导热休克蛋白是困难的。高压氧使HSP的表达略有增加。在吻合口衰竭患者中，凝血因子XIII可增强血清中TGF等生长因子的表达。凝血因子XIII也增加了TGF在体外的表达。术后立即营养支持可增加大鼠体重和结肠吻合口破裂压力。单层的结果是对组织边缘的损伤较小，并保留胶原蛋白网络。床垫缝合线增加了炎症反应和倒置组织的缺血。吻合口愈合的目的可能取决于缝合方法。