Detection of intraabdominal free cancer cells from patients with gastric cancer

胃癌患者腹腔游离癌细胞的检测

• 批准号: 11671236
• 负责人: HIROOKA Yasuaki
• 金额: $ 0.96万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671236/
• 关键词: gastric cancer; washing cytology; Papnicolaou stain; MOC-31 antibody; CEA mRNA; Telomerase activity

1. Detection of intraabdominal free cancer cells by immunohistochemical staining. Immunohistochemical staining of intraoperative peritoneal washing by using MOC- 31 antibody were examed in 71 patients with gastric cancer. In 66 of all cases, restults of immunohistochemical staining were coincident with those of Papnicolaou staining. By looking over the microscopic examination and comparing with the othe immunohistochemical staining (CEA and BerEP4 antibody), accuracy rate of the immunohistochemical staining (MOC-31 antibody) revealed to 98.6%, as compared to 94.4% of Papanicolaou staining, 95.8% of the other immunohistochemical staing (CEA, BerEP4 antibody). Immunohistochemical staining by using MOC-31 antibody is more reliable method to detect intraabdominal free cancer cells than the usual Papnicolaou staining.2. Detection of intraabdominal free cancer cells by RT-PCR method.CEAmRNA of intraabdominal peritoneal washes was measured by means of the RT-PCR method in 44 patients with gastric cancer. In 6 cases which were cytology negative, CEAtmRNA proved to be detected. RT-PCR is revealed to be more reliable methd to detect the free cancer cells than usual Papnicolaou staining.3. Detection of intraabdominal free cancer cells by means of Telomerase activity. Telomerase activity of intraabdominal peritoneal washe was measured in 58 patients with gastric cancer. In 24 of all cases, results of Teromerase activity were not coincident with those of Papnicolaou staining. Telomerase activity is revealed to be not so reliable method to detect the free cancer cells than usual Papnicolaou staining.

1.腹腔游离癌细胞的免疫组化检测。应用MOC- 31抗体对71例胃癌患者术中腹腔冲洗液进行免疫组化染色。其中66例免疫组化结果与巴氏染色结果一致。通过与其他免疫组化染色（CEA和BerEP 4抗体）比较，免疫组化染色（MOC-31抗体）的准确率为98.6%，巴氏染色为94.4%，其他免疫组化染色（CEA、BerEP 4抗体）为95.8%。MOC-31抗体免疫组化染色是检测腹腔内游离癌细胞较常规巴氏染色更可靠的方法. RT-PCR法检测腹腔游离癌细胞应用RT-PCR法检测44例胃癌患者腹腔冲洗液中癌细胞抗原（CEAmRNA）。6例细胞学检查阴性者CEAtmRNA阳性。RT-PCR检测游离癌细胞比常规巴氏染色更可靠.端粒酶活性检测腹腔游离癌细胞对58例胃癌患者腹腔冲洗液进行端粒酶活性检测。24例端粒酶活性与巴氏染色结果不一致。研究表明，与常规巴氏染色相比，端粒酶活性检测游离癌细胞的方法并不那么可靠。

项目成果

広岡保明: "胃癌の術中腹腔内洗浄細胞診"臨床検査. 44(11). 1315-1318 (2000)

Yasuaki Hirooka：“胃癌的术中腹腔灌洗细胞学”临床检查44（11）（2000）。

広岡保明: "胃癌における術中腹腔内洗浄細胞診"日本臨床細胞学会雑誌. 38. 80 (1999)

Yasuaki Hirooka：“胃癌的术中腹腔灌洗细胞学”日本临床细胞学会杂志 38. 80 (1999)。

Hirooka, Y et al.: "Intraoperative peritoneal washing cytology for the patients with gastric cancer.(in japanese)"RINSHOKENSA. 44(11). 1315-1318 (2000)

Hirooka, Y 等人：“胃癌患者的术中腹膜冲洗细胞学检查。（日语）”RINSHOKENSA。

広岡保明: "予後因子としての胃癌腹腔内洗浄細胞診"日本消化器外科学会雑誌. 32. 239 (1999)

Yasuaki Hirooka：“胃癌腹腔灌洗细胞学检查作为预后因素”日本胃肠外科杂志 32. 239 (1999)。

広岡保明: "胃癌の術中腹腔内洗浄細胞診"臨床検査. 44. 1315-1318 (2000)

Yasuaki Hirooka：“胃癌的术中腹腔灌洗细胞学检查”临床检查44。1315-1318（2000）。