Prophylactic effect of proton pump inhibitor in the development of bronchiolitis obliterans after lung transplantation in rats

背景和目标。闭塞性细支气管炎是影响同种异体肺移植受者延长生存期的最重要并发症。从最初的结果到最终的病理实体的演变在很大程度上是未知的。本研究旨在评价质子泵抑制剂对大鼠肺移植术后闭塞性细支气管炎的影响。方法（实验方法1）：将褐挪威大鼠供体肺移植至褐挪威大鼠（同种异体移植，A组）或Lewis大鼠（同种异体移植，b组）。b组大鼠给予环孢素，于移植后3个不同时间点（移植后30、60、90天）取两组移植肺，用硝基酪氨酸进行免疫组化研究，检测闭塞性细支气管炎。方法（实验方式2）：将褐挪威大鼠气管段移植至褐挪威大鼠（同种异体移植，A组）或Lewis大鼠（同种异体移植，B组）。移植物被植入皮下袋和腹部大网膜包裹。B组大鼠给予质子泵抑制剂（200μ g/kg/d，巴菲霉素A1） 21d。结果与结论：实验1：硝基酪氨酸免疫组化染色显示同种异体移植术后90天出现闭塞性细支气管炎。Exp。2。B组大鼠给予质子泵抑制剂可抑制纤维性气道闭塞的发展。然而，在我们的研究中，这个实验模型并不适合评估质子泵抑制剂的疗效，因为在发展中的BO发生率并不稳定。

Background and Objectives. Bronchiolitis obliterans is the most significant complication adversely affecting prolonged survival of lung allograft recipients. The evolution from the initial isult to the final pathologic entity is largely unknow. The aim of this study was to evaluate the efficacy of proton pump inhibitor in the development of bronchiolitis obliterans after lung transplantation in rats.Methods(Experimental stydy 1) : Donor lung were transplanted from Brown Norway rats to Brown Norway rats (isografts ; group A) or to Lewis rats (allograft ; group B. Cyclosporine was administrated into rats of group B. Implanted lung in both groups were harvested at 3 different time points (from 30, 60 and 90 day after transplantation) and assessed immunohistochemical study using nitrotyrosin for detecting of bronchiolitis obliterans.Methods(Experimental stydy 2) : Tracheal segments were transplanted from Brown Norway rats to Brown Norway rats (isografts ; group A) or to Lewis rats (allograft ; group B). Grafts were implanted into a subcutaneous pouch and an abdominal omental wrap. Proton pump inhibitor (200μ g/kg/day of bafilomycin A1) was administrated into rats of group B for 21days.Results and Conclusions : Exp. 1 : Immunohistochemical staining using nitrotyrosin showed a developing of bronchiolitis obliterans in allograft 90 days after transplantation. Exp. 2. The development of fibrous airway obliteration was inhibited in group B rat which was administered proton pump inhibitor. However, This experimental model was not preferable for evaluating the efficacy of proton pump inhibitor in our study because the rate of occurrence in developing BO was not stable.