Experimental study about efficacy of treatment for metastatic lung tumor using Matrix Metalloproteinasa Inhibitor An experimental study of pulmonary artery infusion with cisplatin for solitary pulmonary tumor model using rat colorectal adenocarcinoma cell

基质金属蛋白酶抑制剂治疗转移性肺肿瘤疗效的实验研究 顺铂肺动脉灌注大鼠结直肠腺癌细胞孤立性肺肿瘤模型的实验研究

• 批准号: 09671400
• 负责人: SAITO Yukihito
• 金额: $ 2.3万
• 依托单位: KANSAI MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671400/
• 关键词: pulmonary artery infusion; lung tumor; open lung injection; MMP; MMPインヒビター; 転移性肺腫瘍; vital microslope; 生体顕微鏡

Objectives : The purpose of this study is to assess an availability of the tumor model made by open lung injection for an experiment about metastatic lung tumor by confirmation of its blood supply, and to evaluate an efficacy of pulmonary artery infusion (PAI) with cisplatin in this model using rat colorectal adenocarcinoma (RCN-9) cell line.Methods : Male F344 rats were used. In experiment 1, rat colorectal adenocarcinoma (RCN-9) cells were injected to the rat left lung on day 0. On day 10, tissue blood flow of the tumors before/after pulmonary arterial occlusion were measured. I experiment 2, RCN-9 cells were injected to the rat left lung, and no other treatment was performed in the control group. The other group was performed PAL with 0.1 mg of cisplatin on day 3. On day 10, all rats were sacrificed and measured the maximum cross sectional areas of the tumors. Results : In experiment 1, the tissue blood flow of the tumors was significantly decreased after the pulmonary. arterial occlusion. In experiment 2, the maximum cross sectional areas of the tumors in the PAI group were significantly lower than those in the control group.Conclusion : The blood flow of the tumor is supplied from pulmonary artery, so this model seems to be useful for the experiment about metastatic lung tumor. The tumor sizes were significantly reduced with PAI in this model. The local chemotherapy with PAI could be an effective choice of therapy for metastatic lung tumor from colorectal adenocarcinoma.

目的：以大鼠结直肠腺癌(RCN-9)细胞株为实验对象，通过确定肺内血供，评价开胸肺内注射肿瘤模型的可行性，并评价顺铂(PAI)对该模型的疗效。实验一将大鼠结肠腺癌(RCN-9)细胞接种于第0天大鼠左肺内。于第10天测定肺动脉阻断前后肿瘤的组织血流量。实验二：将RCN-9细胞注入大鼠左肺，对照组不作其他处理。另一组于第3天给予顺铂0.1 mg，于第10天处死所有大鼠，测量肿瘤最大横截面积。结果：实验1肺切除后肿瘤组织血流量明显减少。动脉闭塞。在实验2中，PAI组肿瘤的最大横截面积明显低于对照组。结论：肿瘤的血流来自于肺动脉，该模型可用于肺转移瘤的实验研究。在该模型中，PAI可显著缩小肿瘤大小。PAI局部化疗可作为结直肠癌肺转移瘤治疗的有效选择。