Experimental study about efficacy of treatment for metastatic lung tumor using Matrix Metalloproteinasa Inhibitor An experimental study of pulmonary artery infusion with cisplatin for solitary pulmonary tumor model using rat colorectal adenocarcinoma cell

目的：本研究的目的是通过肺开放注射法建立肺转移瘤模型，以确定其血供，并以大鼠结肠直肠腺癌（RCN-9）细胞系为模型，评价肺动脉灌注顺铂（派）对肺转移瘤的治疗效果。在实验1中，在第0天将大鼠结肠直肠腺癌（RCN-9）细胞注射到大鼠左肺。在第10天，测量肺动脉阻断前后肿瘤的组织血流。在实验2中，将RCN-9细胞注射到大鼠左肺，对照组不进行其他处理。另一组在第3天用0.1 mg顺铂进行PAL。在第10天，处死所有大鼠并测量肿瘤的最大横截面积。结果：实验1中，肺动脉栓塞后肿瘤组织血流量明显减少。动脉闭塞实验2中，派组肿瘤最大截面积明显低于对照组。结论：该模型可用于肺转移瘤的实验研究。在该模型中，派显著减小肿瘤大小。派局部化疗是治疗结直肠腺癌肺转移的有效方法。

Objectives : The purpose of this study is to assess an availability of the tumor model made by open lung injection for an experiment about metastatic lung tumor by confirmation of its blood supply, and to evaluate an efficacy of pulmonary artery infusion (PAI) with cisplatin in this model using rat colorectal adenocarcinoma (RCN-9) cell line.Methods : Male F344 rats were used. In experiment 1, rat colorectal adenocarcinoma (RCN-9) cells were injected to the rat left lung on day 0. On day 10, tissue blood flow of the tumors before/after pulmonary arterial occlusion were measured. I experiment 2, RCN-9 cells were injected to the rat left lung, and no other treatment was performed in the control group. The other group was performed PAL with 0.1 mg of cisplatin on day 3. On day 10, all rats were sacrificed and measured the maximum cross sectional areas of the tumors. Results : In experiment 1, the tissue blood flow of the tumors was significantly decreased after the pulmonary. arterial occlusion. In experiment 2, the maximum cross sectional areas of the tumors in the PAI group were significantly lower than those in the control group.Conclusion : The blood flow of the tumor is supplied from pulmonary artery, so this model seems to be useful for the experiment about metastatic lung tumor. The tumor sizes were significantly reduced with PAI in this model. The local chemotherapy with PAI could be an effective choice of therapy for metastatic lung tumor from colorectal adenocarcinoma.