Experimental study about efficacy of treatment for metastatic lung tumor using Matrix Metalloproteinasa Inhibitor An experimental study of pulmonary artery infusion with cisplatin for solitary pulmonary tumor model using rat colorectal adenocarcinoma cell

目的：本研究通过确定肺开孔注射肿瘤模型的血供情况，评估其用于转移性肺肿瘤实验的可行性，并以大鼠结直肠癌（RCN-9）细胞系为实验对象，评价顺铂肺动脉灌注（PAI）在该模型中的疗效。方法：选用雄性F344大鼠。实验1，第0天将大鼠结肠直肠腺癌（RCN-9）细胞注射到大鼠左肺。第10天，测量肺动脉闭塞前后肿瘤组织血流量。实验二，将RCN-9细胞注射到大鼠左肺，对照组不做其他处理。另一组在第3天给予顺铂0.1 mg的PAL治疗。第10天处死大鼠，测定肿瘤的最大横截面积。结果：实验1肿瘤组织血流量明显减少。动脉闭塞。实验2中，PAI组肿瘤的最大横截面积明显低于对照组。结论：肿瘤血流由肺动脉供血，该模型可用于肺转移瘤的实验。PAI能显著降低肿瘤的大小。PAI局部化疗是治疗结肠腺癌转移性肺肿瘤的有效选择。

Objectives : The purpose of this study is to assess an availability of the tumor model made by open lung injection for an experiment about metastatic lung tumor by confirmation of its blood supply, and to evaluate an efficacy of pulmonary artery infusion (PAI) with cisplatin in this model using rat colorectal adenocarcinoma (RCN-9) cell line.Methods : Male F344 rats were used. In experiment 1, rat colorectal adenocarcinoma (RCN-9) cells were injected to the rat left lung on day 0. On day 10, tissue blood flow of the tumors before/after pulmonary arterial occlusion were measured. I experiment 2, RCN-9 cells were injected to the rat left lung, and no other treatment was performed in the control group. The other group was performed PAL with 0.1 mg of cisplatin on day 3. On day 10, all rats were sacrificed and measured the maximum cross sectional areas of the tumors. Results : In experiment 1, the tissue blood flow of the tumors was significantly decreased after the pulmonary. arterial occlusion. In experiment 2, the maximum cross sectional areas of the tumors in the PAI group were significantly lower than those in the control group.Conclusion : The blood flow of the tumor is supplied from pulmonary artery, so this model seems to be useful for the experiment about metastatic lung tumor. The tumor sizes were significantly reduced with PAI in this model. The local chemotherapy with PAI could be an effective choice of therapy for metastatic lung tumor from colorectal adenocarcinoma.