The research on the bone resorption after artificial joint replacement by the high density polyethylene abrasion powder.-using op/op mice-

用无巨噬细胞集落刺激因子(M-CSF)活性的OP/OP小鼠和有M-CSF活性的小鼠对照观察羟基磷灰石颗粒诱导的骨吸收情况，在两组小鼠的顶骨上形成毛刺孔，植入直径小于或等于10μm的高密度聚乙烯颗粒。在选定的时间段后暴露移植部位，进行HE染色观察，酒石酸抗性酸性磷酸酶(TRAP)染色鉴定破骨细胞，F4/80和Mac2抗小鼠巨噬细胞抗体鉴定巨噬细胞，并进行透射电子显微镜观察。在HE染色中，正常小鼠、OP/OP小鼠的单个核细胞中也可识别出大量的HDP颗粒。在正常小鼠中，F4/80、Mac2抗体染色和TRAP染色均呈阳性(但TRAP阳性细胞很少)。在OP/OP小鼠中，虽然Mac2抗体染色为阳性，但阳性细胞不能同时识别F4/80抗体染色和TRAP染色。在电子显微镜下观察，加入HDP颗粒的细胞具有明显的成纤维细胞的形态特征。目前的研究表明，不仅巨噬细胞，成纤维细胞也主动摄取HDP颗粒，说明成纤维细胞在骨吸收中起着重要的作用。且TRAP阳性，可见单个核细胞摄取HDP，未见破骨细胞或破骨前细胞有吞噬作用的报道，因此，在考虑破骨细胞在骨吸收中的作用时，这是一个新的认识。

Using op/op mice without the macrophage-colony stimulating factor (M-CSF) activity and litter normal mice with the M-CSF activity, comparison examination on the bone resorption of HDP particles induced was carried out.Burr hole was produced in the parietal bone of these 2 groups, and the high density polyethylene particles (HDP, 10 μm diameter or less) were transplanted. The graft place was exposed after the selected time period, and the observation by H.E staining, identification of osteoclast by tartaric acid resistance acidity phosphatase (TRAP) staining and macrophage by F4/80 and Mac2 antimouse macrophage antibody and transmission electron microscopic observation were carried out. In the H.E staining, the mononuclear cells in which normal mice, op/op mice also took in HDP particles particles were recognized in great numbers. In the normal mice, anyway showed positive, when F4/80, Mac2 antibody staining and TRAP staining were observed (however, the TRAP positive cell are very few). In the op/op mice, though the Mac2 antibody staining was the positive, the positive cells could not be together recognized F4/80 antibody staining and TRAP staining. In the electron microscopic observation, the cells which took in HDP particles showed clearly morplological features of the fibroblast. And the uptakes of HDP particles by macrophage was also able to be observed in the normal mice.From present examination, it was proven that not only macrophage but also fibroblast also actively take in HDP particles, and it was indicated that fibroblast played an important role for the bone resorption. And TRAP positive, mononuclear cell which took in HDP was observed, there is no report which osteoclast or pre-osteoclast have phagocytosis, so that is a new knowledge, when the role of osteoclast in the bone resorption is considered.