Tissue-engineered cartilage using cultured chondrocytes and reinforced with a periosteal explant for repair of articular cartilage

利用组织工程化软骨治疗关节软骨损伤一直受到关注。然而，它的临床应用受到了挑战，因为它很难与软骨的受损区域结合，并且与下面的骨结合不充分。作为克服这些缺点的一种手段，我们使用组织工程软骨与骨膜外植体增强，进行组织学和生物化学评价，并显示初始的体内数据创建了一个复合移植物。将培养的源自比格犬关节软骨的软骨细胞接种到聚乙醇酸非织造网上，其中将从同一比格犬中取出的新鲜骨膜外植体缝合到一侧。在培养皿中生长的细胞的三维培养导致透明软骨的构建，其中一侧附着于骨膜外植体。在工程化软骨与骨膜移植物结合处观察到骨膜移植物的软骨形成。将自体移植结构植入髋臼再生关节软骨的全层缺损中。由透明软骨和骨膜植块组成的双层复合组织结构可以提供一种简单有效的骨软骨移植修复关节软骨。

The use of tissue-engineered cartilage in the treatment of articular cartilage damage has been receiving attention. However, its clinical application has been challenged by the difficulty in its binding to the damaged area of the cartilage and its insufficient union with the underlying bone. As a means to overcome the drawbacks, we created a composite graft using tissue-engineered cartilage reinforced with a periosteal explant, conducted histological and biochemical evaluations, and showed initial in vivo data. The cultured chondrocytes derived from an articular cartilage of beagle were seeded onto a polyglycolic acid non-woven mesh with a fresh periosteal explant removed from the same beagle sutured to one side. Three-dimensional culture of the cells grown in petri dishes led to the construction of a hyaline cartilage with one side attached to a periosteal explant. Chondrogenesis of the periosteal explant was observed at the junction where the engineered cartilage was reinforced with a periosteal explant. The autologously grafted construct into a full-thickness defect on the acetabulum regenerated articular cartilage. The two-layered composite tissue construct consisting of a hyaline cartilage and a periosteal explant could provide a simple and effective osteochondral graft for articular cartilage repair.