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The effects of inhalational anesthetics and NO upon activities of spinal dorsal horn neuron

吸入麻醉药和NO对脊髓背角神经元活动的影响

基本信息

批准号：
11671480
负责人：
IDE Yasuo
金额：
$ 2.37万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2002
项目状态：
已结题

项目摘要

[background] Nitric oxide(NO) have a lot of actions in various tissues and organs. It is controversial about the action of NO with Inhalational anesthetics. We will investigate The effects of inhalational anesthetics and NO upon activities of spinal dorsal horn neuron.[method] Male Spargue-Dawley rats were anesthetized with halothane. Following tracheotomy, the animals were mechanically ventilated. Catheters were inserted into veins and arteries and the animal was decerebrated by aspiration and the spinal cord was transected at L1 and L2 and laminectomy was made. Animals were fixed on a stereotaxic frame. Following surgery, ventilation was maintained by pure oxygen. Extra-cellular single-cell action potentials were recorded and spike firing rate were measured. The low-threshold-mechanoreceptive neuron, nociceptor-specific neuron, wide-dynamic-range neuron was identified by the modality and by the depth from the cord dorsum. Four groups were studied : 1. Under pure oxygen, NO synthesis accelerator : L-arginine administrated group 2. Under pure oxygen, NO synthesis Inhibitor : L-NAME administrated group 3. Under 1MAC Halothane(1.1%), NO synthesis accelerator: L-arginine administrated group 4. Under 1MAC Halothane(1.1%), NO synthesis Inhibitor : L-NAME administrated group.[Results] L- NAME had no effect on the single-unit activity of dorsal horn neurons, and L-arginine had noeffects on the single-unit activity of dorsal horn neurons.[Conclusion] Our results suggest that NO had no action between the anesthetic mechanisms of Inhalational anesthetics.

[背景]一氧化氮（NO）在多种组织器官中具有多种作用。吸入麻醉药对NO的作用存在争议。本实验旨在研究吸入麻醉药和一氧化氮对脊髓背角神经元活动的影响。[方法]雄性Spargue-Dawley大鼠氟烷麻醉。气管切开术后，对动物进行机械通气。将导管插入静脉和动脉，通过抽吸使动物去大脑，在L1和L2处横断脊髓，并进行椎板切除术。将动物固定在立体定位架上。手术后，通过纯氧维持通气。记录细胞外单细胞动作电位并测量锋电位发放率。根据形态和距离脊髓背侧的深度鉴定出低阈值机械感受神经元、伤害感受器特异性神经元、宽动态范围神经元。研究了四组：1。纯氧条件下，NO合成促进剂：L-精氨酸组2。纯氧条件下，NO合成抑制剂：L-NAME组3。在1 MAC氟烷（1.1%）下，NO合成促进剂：L-精氨酸组4。在1 MAC氟烷（1.1%）下，NO合成抑制剂：L-NAME给药组。[结果] L-NAME对脊髓背角神经元的单位放电无影响，L-Arg对脊髓背角神经元的单位放电无影响。[结论] NO在吸入麻醉药的麻醉机制中没有作用。