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Investigation the mechanism of renal stone formation by used the MDCK cell line.

利用MDCK细胞系研究肾结石形成的机制。

基本信息

批准号：
11671547
负责人：
OHTAWARA Yosihisa
金额：
$ 2.37万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671547/
关键词：
MDCK cell oxalic acid calculus renal tubular epithelial cells nephrolith phosphoric acid calculus UIT PGE2 vit. K 腎結石シュウ酸結石 UTI 尿路結石ショウ酸結石オステオポンチン

项目摘要

This study elucidates a cause of the calculus formation by making a model of humans nephrolith with a cultured cell. However, I was careless by making of an oxalic acid calculus.Model making of an oxalic acid calculus uses animal experiment together simultaneously, and a thought colander will not get transplant to an animal of MDCK cells. The renal tubular epithelial cells of a different kind animal was used in addition to a MDCK cells by this study, and the difference was examined, but the generation of a phosphoric acid calcium calculus was specifically watched only to a MDCK cell. We think that this participates in specific calcium ion transportation ability of this cell. It was suggested that vit. K and PGE2 participated in accumulation of the calcium for stone formation.As a result of having measured the UIT density in urine from a calculus patient of humans, we thought that it was effective in the UIT for calculus prevention.The osteopontin density known as one of a cause of calculus form was measured during urine of humans calculus patient, and we thought to be a promotion factor of the calculus formation.The calculus ingredient which a MDCK cell generates is different from oxalic acid calcium, and examination of these restraint factors and a promotion factor is not done enough this time because it is phosphoric acid calcium. If it is possible, a model forming an oxalic acid calcium calculus in this cell is done sequentially in future, and it is necessary to make clear it about these factors.

这项研究通过用培养细胞制作人类肾结石模型来阐明结石形成的原因。然而，我在制作草酸结石时大意了。草酸结石的模型制作同时使用了动物实验，并且认为漏勺不会将MDCK细胞移植到动物身上。本研究中，除了MDCK细胞以外，还使用不同种类的动物的肾小管上皮细胞，考察其差异，但仅针对MDCK细胞特异性地观察磷酸钙结石的产生。我们认为这参与了该细胞的特定钙离子运输能力。有人建议维特。 K和PGE2参与结石形成的钙的积累。通过测量人类结石患者尿液中的UIT密度，我们认为它对于预防结石是有效的。在人类结石患者的尿液中测量了被称为结石形成原因之一的骨桥蛋白密度，我们认为这是结石形成的促进因素。 MDCK细胞生成的成分与草酸钙不同，由于是磷酸钙，所以这次对这些抑制因素和促进因素的研究还不够充分。如果可能的话，今后将在该细胞内依次进行形成草酸钙结石的模型，有必要明确这些因素。