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Investigation the mechanism of renal stone formation by used the MDCK cell line.

利用MDCK细胞系研究肾结石形成的机制。

基本信息

批准号：
11671547
负责人：
OHTAWARA Yosihisa
金额：
$ 2.37万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671547/
关键词：
MDCK cell oxalic acid calculus renal tubular epithelial cells nephrolith phosphoric acid calculus UIT PGE2 vit. K 腎結石シュウ酸結石 UTI 尿路結石ショウ酸結石オステオポンチン

项目摘要

This study elucidates a cause of the calculus formation by making a model of humans nephrolith with a cultured cell. However, I was careless by making of an oxalic acid calculus.Model making of an oxalic acid calculus uses animal experiment together simultaneously, and a thought colander will not get transplant to an animal of MDCK cells. The renal tubular epithelial cells of a different kind animal was used in addition to a MDCK cells by this study, and the difference was examined, but the generation of a phosphoric acid calcium calculus was specifically watched only to a MDCK cell. We think that this participates in specific calcium ion transportation ability of this cell. It was suggested that vit. K and PGE2 participated in accumulation of the calcium for stone formation.As a result of having measured the UIT density in urine from a calculus patient of humans, we thought that it was effective in the UIT for calculus prevention.The osteopontin density known as one of a cause of calculus form was measured during urine of humans calculus patient, and we thought to be a promotion factor of the calculus formation.The calculus ingredient which a MDCK cell generates is different from oxalic acid calcium, and examination of these restraint factors and a promotion factor is not done enough this time because it is phosphoric acid calcium. If it is possible, a model forming an oxalic acid calcium calculus in this cell is done sequentially in future, and it is necessary to make clear it about these factors.

这项研究通过用培养的细胞制作人类肾结石的模型来阐明结石形成的原因。然而，我制作草酸结石是粗心大意的，草酸结石的模型制作需要同时进行动物实验，思想滤器不会移植到MDCK细胞的动物身上。本研究用另一种动物的肾小管上皮细胞代替MDCK细胞，并对其差异进行了检验，但仅对MDCK细胞特异性地观察了磷酸钙结石的产生。我们认为这可能参与了该细胞特有的钙离子转运能力。有人建议VIT。K和PGE2参与了结石形成过程中钙的积累。通过对一例结石患者尿液中UIT浓度的测定，我们认为它在预防结石的UIT中是有效的。骨桥蛋白密度是结石形成的原因之一，我们认为它是结石形成的促进因素。MDCK细胞产生的结石成分不同于草酸钙，这次对这些制约因素和促进因素的检测做得不够，因为它是磷酸钙。如果可能的话，在这个细胞中形成草酸钙结石的模型将在未来按顺序进行，有必要对这些因素进行明确。