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Morphological Study on Mechanism of Traumatic Cataract

外伤性白内障发生机制的形态学研究

基本信息

批准号：
11671755
负责人：
UGA Shigekazu
金额：
$ 1.28万
依托单位：
Kitasato Unviersity
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Mice lenses were injured at the anterior, lateral and posterior sides by means of a transcorneal or transscleral needle puncture, and the response of the lens was examined morphologically.The lens epithelium, which covers the anterior half of the lens, has a mechanism for repairing any perforating injury. However, deep injuries or large area injuries resulted in the rapid development of opacity. The development of such opacity occurred in two patterns according to the site of liquefaction ; posterior opacity and anterior opacity. To examine the mechanism of the posterior opacity, the tracer was injected into the anterior cortex. The tracer was seen moving toward the posterior side along the periphery of the nucleus.Moreover, it was found that in the rodent lens there was a specific zone called the "perinuclear zone", which is prone to develop opacity in response to an injury.When the lens was injured at the lateral side, its repair function was incomplete and cataractous prognession was rather accelerated. When the lens was injured at the posterior side, the protruded fibers became atrophic after 7 months. However, the wounded cortex remained almost unrepaired, and the lens became opaque by unusual thickening of the anterior cortex and posterior deviation of the nucleus.These findings indicate that the lens becomes cataractous when the anterior injury exceeds the limit of the epithelial repair function, or when the posterior injury disturbed formation of the posterior cortex.

用经角膜或经巩膜针刺伤小鼠晶状体的前、外侧和后三侧，观察晶状体的形态反应。覆盖晶状体前半部分的晶状体上皮具有修复任何穿孔损伤的机制。然而，深度损伤或大面积损伤导致不透明迅速发展。根据液化地点的不同，这种不透明的发展有两种模式；后混浊和前混浊。为了研究后阴影的形成机制，我们将示踪剂注射到前皮层。示踪剂沿核外周向后侧移动。此外，我们还发现啮齿动物的晶状体中有一个叫做“核周区”的特殊区域，在受到损伤后，这个区域很容易出现混浊。当晶状体外侧受损时，晶状体的修复功能不全，白内障的进展速度加快。当晶状体后侧受损伤时，7个月后晶状体突出的纤维萎缩。然而，受损的皮质几乎没有修复，由于前部皮质异常增厚和核的后部偏移，晶状体变得不透明。这些结果表明，当晶状体前部损伤超过上皮修复功能的极限时，或者当后部损伤干扰后皮层的形成时，晶状体就会发生白内障。