Gene transfection in human keratinocyte and melanocyte

人角质形成细胞和黑素细胞的基因转染

• 批准号: 11671780
• 负责人: YAMAMOTO Yuhei
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671780/
• 关键词: transfection; ha-tagged vector; transfectant; lipofection; electroporation; gelsolin; gelsolin truncate; human primary melanoma; マーカー遺伝子; 導入効率; ヒト表皮角化細胞; ヒトメラノサイト系細胞

1 At first, we studied the transfection of ha-tagged human gelsolin cDNA in human squamous carcinomacell line HEC46 and human melanoma cell line in stead of keratinocyte and melanocyte. We used human melanoma tissue and normal skin tissue as a control, and found out a unique 85Kda protein in Western blotting.2 By Western blotting analysis of 38 melanoma samples for the expression of gelsolin using anti-gelsolin antibodies, we identified a new truncated variant of gelsolin p85 (GSNp85) lacking the C-terminal domain, which was always co-expressed with wild type gelsolin. The GSNp85 variant was more frequently expressed in vertical growth phase (VGP) melanomas as compared to radial growth phase (RGP) melanomas and was not expressed in other skin cancers, normal skin tissues, and nevi tissues except for 2 congenital junctional types. These data suggest that the occurrence of GSNp85 in melanoma may be a new indicator for the advanced stage of the tumor.

1起初，我们研究了在人类鳞状癌系HEC46和人类黑色素瘤细胞系中的转染中的HA标记的人凝胶蛋白cDNA，而不是角质类细胞和黑素细胞。 We used human melanoma tissue and normal skin tissue as a control, and found out a unique 85Kda protein in Western blotting.2 By Western blotting analysis of 38 melanoma samples for the expression of gelsolin using anti-gelsolin antibodies, we identified a new truncated variant of gelsolin p85 (GSNp85) lacking the C-terminal domain, which was always co-expressed with wild type凝胶林。与径向生长阶段（RGP）黑色素瘤相比，GSNP85变体在垂直生长阶段（VGP）黑色素瘤中更常见，并且在其他皮肤癌，正常的皮肤组织和NEVI组织中未表达，除2种先天性连接类型外。这些数据表明，黑色素瘤中GSNP85的发生可能是肿瘤晚期阶段的新指标。

项目成果

古川洋志: "悪性黒色腫におけるゲルソリン短縮体の発現と腫瘍浸潤度との関係"北海道医学雑誌. 76巻3号(掲載予定). (2001)

Hiroshi Furukawa：“恶性黑色素瘤中凝溶胶蛋白截短形式的表达与肿瘤侵袭性的关系”《北海道医学杂志》第 76 卷，第 3 期（待出版）。

Furukawa, H.: "Relationship between the Expression of a Gelsolin Truncate and Invasiveness in Malignant Melanoma."Hokkaido Journal of Medical Science. 76 (3)(in press). (2001)

Furukawa, H.：“凝溶胶蛋白截短物的表达与恶性黑色素瘤侵袭性之间的关系。”北海道医学科学杂志。