Development and characterization of rat T helper cell clones specific for Porphyromonas gingivalis-outer membrane protein

牙龈卟啉单胞菌外膜蛋白特异性大鼠 T 辅助细胞克隆的开发和表征

• 批准号: 11671883
• 负责人: SAITO Shigeno
• 金额: $ 2.37万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671883/
• 关键词: Antigen-specific T cell clone; Th2-type T cells; Porphyromonas gingivalis; 抗原特異T細胞株; Th2型細胞; 歯周病; Th2クローン

An association of systemic immune responses to specific oral bacteria can be demonstrated in periodontal disease. Local immune responses can be demonstrated often the putative periodontopathic bacteria can be isolated from active lesions, confirming the infectious nature of the disease and the specificity of the response. An understanding of the local antibody aspects of the interplay between host and infection is emerging. Large number of plasma cells and immunoglobulin-bearing cells are present in the gingivae of periodontitis patients, and immunoglobulins are found in high levels in the gingivae of periodontitis patient. Th1 cells are destructive in periodontal disease and Th2 cells, by virtue of providing help for antibody production, giving rise to considerable increase of antibody, are protective. After immunized rat, this study has developed and characterized Porphyromonas gingivalis outer membrane r40-kDa OMP-specific Th2 clones. There was a significant increase in r40-kDa OMP-specific IGG in supernatant fluid from cultures containing Th2 clone with r40-kDa OMP-primed splenic B cells and r40-kDa OMP.

对特定口腔细菌的系统性免疫反应可以在牙周病中得到证实。局部免疫反应可以被证实，通常假定的牙周病细菌可以从活动性病变中分离出来，证实疾病的传染性和反应的特异性。对宿主和感染之间相互作用的局部抗体方面的理解正在出现。牙周炎患者的牙龈中存在大量浆细胞和携带免疫球蛋白的细胞，牙周炎患者的牙龈中免疫球蛋白水平较高。Th1细胞在牙周病中具有破坏性，而Th2细胞由于有助于抗体的产生，引起抗体的大量增加，具有保护性。本研究在免疫大鼠后，建立并鉴定了牙龈卟啉单胞菌外膜r40-kDa omp特异性Th2克隆。在含有r40-kDa OMP引物的Th2克隆脾B细胞和r40-kDa OMP培养的上清液中，r40-kDa OMP特异性IGG显著增加。