Development and characterization of rat T helper cell clones specific for Porphyromonas gingivalis-outer membrane protein

在牙周病中可以证明对特定口腔细菌的系统免疫反应的相关性。局部免疫反应通常可以从活动性病变中分离出假定的牙周病细菌，证实了疾病的传染性和反应的特异性。对宿主和感染之间相互作用的局部抗体方面的理解正在形成。牙周炎患者牙周组织中存在大量浆细胞和免疫球蛋白阳性细胞，免疫球蛋白在牙周炎患者牙龈中含量较高。Th1细胞在牙周病中具有破坏性，而Th2细胞通过帮助抗体的产生而起到保护作用，从而引起抗体的大量增加。免疫大鼠后，本研究建立并鉴定了牙龈卟啉单胞菌外膜R40-kDa OMP特异性Th2克隆。经R40-kDa OMP诱导的脾B细胞和R40-kDa OMP诱导的Th2克隆培养上清液中R40-kDa OMP特异性IgG显著增加。

An association of systemic immune responses to specific oral bacteria can be demonstrated in periodontal disease. Local immune responses can be demonstrated often the putative periodontopathic bacteria can be isolated from active lesions, confirming the infectious nature of the disease and the specificity of the response. An understanding of the local antibody aspects of the interplay between host and infection is emerging. Large number of plasma cells and immunoglobulin-bearing cells are present in the gingivae of periodontitis patients, and immunoglobulins are found in high levels in the gingivae of periodontitis patient. Th1 cells are destructive in periodontal disease and Th2 cells, by virtue of providing help for antibody production, giving rise to considerable increase of antibody, are protective. After immunized rat, this study has developed and characterized Porphyromonas gingivalis outer membrane r40-kDa OMP-specific Th2 clones. There was a significant increase in r40-kDa OMP-specific IGG in supernatant fluid from cultures containing Th2 clone with r40-kDa OMP-primed splenic B cells and r40-kDa OMP.