Development and characterization of rat T helper cell clones specific for Porphyromonas gingivalis-outer membrane protein

在牙周病中可以证明系统免疫应答与特定口腔细菌的关联。局部免疫反应可以被证明，通常可以从活动性病变中分离出假定的牙周病细菌，从而证实疾病的感染性和反应的特异性。对宿主和感染之间相互作用的局部抗体方面的理解正在出现。牙周炎患者的牙龈中存在大量浆细胞和免疫球蛋白细胞，并且在牙周炎患者的牙龈中发现高水平的免疫球蛋白。Th 1细胞在牙周病中是破坏性的，而Th 2细胞凭借为抗体产生提供帮助，引起抗体的显著增加，是保护性的。免疫大鼠后，本研究开发和表征牙龈卟啉单胞菌外膜r40-kDa外膜蛋白特异性Th 2克隆。在含有Th 2克隆与r40-kDa OMP-致敏的脾B细胞和r40-kDa OMP的培养物的上清液中，r40-kDa OMP-特异性IgG显著增加。

An association of systemic immune responses to specific oral bacteria can be demonstrated in periodontal disease. Local immune responses can be demonstrated often the putative periodontopathic bacteria can be isolated from active lesions, confirming the infectious nature of the disease and the specificity of the response. An understanding of the local antibody aspects of the interplay between host and infection is emerging. Large number of plasma cells and immunoglobulin-bearing cells are present in the gingivae of periodontitis patients, and immunoglobulins are found in high levels in the gingivae of periodontitis patient. Th1 cells are destructive in periodontal disease and Th2 cells, by virtue of providing help for antibody production, giving rise to considerable increase of antibody, are protective. After immunized rat, this study has developed and characterized Porphyromonas gingivalis outer membrane r40-kDa OMP-specific Th2 clones. There was a significant increase in r40-kDa OMP-specific IGG in supernatant fluid from cultures containing Th2 clone with r40-kDa OMP-primed splenic B cells and r40-kDa OMP.