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Opsonophagocytic effect of antibody against recombinant 40-kDa OMP of P.gingivalis on neutrophils and induction of Th1 immune responses

牙龈卟啉单胞菌重组 40-kDa OMP 抗体对中性粒细胞的调理吞噬作用和诱导 Th1 免疫反应

基本信息

批准号：
09671935
负责人：
SAITO Shigeno
金额：
$ 1.92万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Porphyromonas gingivalis is associated with the initiation and progression of adult periodontitis. The outer membrane proteins of the bacteria are potentially important targets for interaction with host defense systems. A 40-kDa OMP is conserved among many strains of P.gingivalis. For the development of recombinant 40-kDa OMP as a vaccine for passive immunization, the elucidation of the roles of the anti-r40-kDa OMP antibody in the host defense against P.gingivalis is essential. We demonstrated that r40-kDa OMP antibody in the presence of human complement successfully opsonized [^3H]-thymidine-labelled P.gingivalis as a target for phagocytosis by HL-60 cells differentiated with DMSO.The phagocytized bacteria were then intracellularly killed and lysed, and the radioactive degradation debris egested into the culture medium. We conclude that an antibody against r40-kDa OMP has an opsonic activity on human neutrophil function for phagocytosis of P.gingivalis. We might speculate that Th2 cells can be protective via the provision of help for antibody synthesis and that Th1 and CD8^+ T lymphocytes might be destructive via the prominent production of IFN-gamma and the potential stimulation of macrophage secretion of IL-1 and subsequent bone-destructive activity. Thus, T-cell features such as help[for antibody are important modulators of periodontal inflammation and therefore may be considered targets for disease intervention.

牙龈卟啉单胞菌与成人牙周炎的发生和发展有关。细菌的外膜蛋白是与宿主防御系统相互作用的潜在重要靶标。一个40 kDa的外膜蛋白是保守的许多菌株的牙龈卟啉单胞菌。为了开发重组40-kDa OMP作为被动免疫的疫苗，必须阐明抗r40-kDa OMP抗体在宿主防御牙龈卟啉单胞菌中的作用。我们证明，在人补体存在下，r40-kDa OMP抗体成功调理[^3H]-胸苷标记的牙龈卟啉单胞菌，使其成为DMSO分化的HL-60细胞吞噬的靶点，被吞噬的细菌随后在细胞内被杀死并裂解，放射性降解碎片排泄到培养基中。我们的结论是，抗r40-kDa外膜蛋白的抗体具有调理活性对人类中性粒细胞功能的牙龈卟啉单胞菌的吞噬作用。我们可以推测，Th 2细胞可以通过帮助抗体合成来起到保护作用，而Th 1和CD 8 ^+ T淋巴细胞则可能通过显著产生IFN-γ和潜在刺激巨噬细胞分泌IL-1以及随后的骨破坏活性来起到破坏作用。因此，T细胞的特征，如帮助抗体是牙周炎症的重要调节剂，因此可以被认为是疾病干预的目标。