Opsonophagocytic effect of antibody against recombinant 40-kDa OMP of P.gingivalis on neutrophils and induction of Th1 immune responses

牙龈卟啉单胞菌与成人牙周炎的发生和发展有关。细菌的外膜蛋白是与宿主防御系统相互作用的潜在重要靶点。一个40 kda的OMP在许多菌株中是保守的。为了开发重组40-kDa OMP作为被动免疫疫苗，阐明抗r40- kda OMP抗体在宿主防御牙龈假单胞菌中的作用至关重要。我们证明r40-kDa OMP抗体在人补体存在下成功地使[^3H]-胸腺嘧啶标记的牙龈卟啉卟啉被DMSO分化的HL-60细胞吞噬。被吞噬的细菌在细胞内被杀死和裂解，放射性降解碎片被排出到培养基中。我们得出结论，抗r40-kDa OMP的抗体对人中性粒细胞吞噬牙龈假单胞菌的功能有抑制作用。我们可以推测Th2细胞可以通过提供抗体合成的帮助而起到保护作用，而Th1和CD8^+ T淋巴细胞可能通过ifn - γ的显著产生和巨噬细胞IL-1分泌的潜在刺激以及随后的骨破坏活性而具有破坏性。因此，t细胞的特征，如帮助抗体是牙周炎症的重要调节因子，因此可能被认为是疾病干预的目标。

Porphyromonas gingivalis is associated with the initiation and progression of adult periodontitis. The outer membrane proteins of the bacteria are potentially important targets for interaction with host defense systems. A 40-kDa OMP is conserved among many strains of P.gingivalis. For the development of recombinant 40-kDa OMP as a vaccine for passive immunization, the elucidation of the roles of the anti-r40-kDa OMP antibody in the host defense against P.gingivalis is essential. We demonstrated that r40-kDa OMP antibody in the presence of human complement successfully opsonized [^3H]-thymidine-labelled P.gingivalis as a target for phagocytosis by HL-60 cells differentiated with DMSO.The phagocytized bacteria were then intracellularly killed and lysed, and the radioactive degradation debris egested into the culture medium. We conclude that an antibody against r40-kDa OMP has an opsonic activity on human neutrophil function for phagocytosis of P.gingivalis. We might speculate that Th2 cells can be protective via the provision of help for antibody synthesis and that Th1 and CD8^+ T lymphocytes might be destructive via the prominent production of IFN-gamma and the potential stimulation of macrophage secretion of IL-1 and subsequent bone-destructive activity. Thus, T-cell features such as help[for antibody are important modulators of periodontal inflammation and therefore may be considered targets for disease intervention.