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Evaluation of mental stresses during dental treatments by chromogranin A in saliva

用唾液中的嗜铬粒蛋白A评价牙科治疗期间的精神压力

基本信息

批准号：
11671891
负责人：
UNO Shigeru
金额：
$ 2.24万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Chromogranin A (CgA) is co-released with catecholamines, mediators in adrenargic neurons of sympathetic nervous system. The purpose of the study is to establish the measurement of salivary CgA and to evaluate the relationship between CgA and metal stresses during dental treatments.Enzyme immunoassay (EIA) for rat CgA was developed (Yanaihara Institute), and used to estimate CgA-like immonoreactivity (CgA-like IR) in saliva secreted from the salivary duct of isolated vascularly perfused rat submandibular gland. Approxirnately 1 mM of CgA-like IR is secreted into saliva in response to stimulation with 3 μM phenylephrine, an α-adrenergic agonist. The secretory responses were scarcely inhibited in Ca^<2+>-deficient environment, and were almost completely inhibited by 100 μM 2-aminoethoxydiphenyl borate (2-APB). These results indicate that mobilization of Ca^<2+> from the IP_3 sensitive pool may preferentially be involved in the secretory responses to α-adrenargic agonist. The secretory res … More ponses to isoproterenol, β-adrenergic agonist, remained almost unchanged in the Ca^<2+>-deficient environment, and were markedly delayed by 100μM 2-APB but were regained gradually after removal of 2-APB and the agonist. Mobilization of Ca^<2+> from the pool may also be involved to facilitate synergistic signallings in the β-adrenergic responses. Results obtained show that CgA-like IR secretion into saliva may be an index of activity of the sympathetic nerve, especially α-adrenergic activity, and suggest a possible intracellular signalling mechanism in the adrenergic responses of the granulated duct cells in rat submandibular glands.In six healthy adults, salivary CgA was determined (Hokkaido Univ., Dental Hospital). Whole saliva was sampled during dental treatments such as dental scaring, then subjected to determine CgA using the kit. The mean CgA was 6.53 pmol/mg protein. The CgA value was varied during treatments. But there was no clear relation between CgA values, blood pressure, pulse and degree of mental stress. Less

嗜铬粒蛋白A（CgA）是交感神经系统肾上腺素能神经元中与儿茶酚胺类介质共同释放的一种蛋白质。本研究的目的是建立唾液CgA的测量和评估CgA和金属应力之间的关系在牙科治疗过程中，开发了大鼠CgA的酶免疫测定（EIA）（Yanaihara Institute），并用于评估从离体血管灌注的大鼠下颌下腺的唾液导管分泌的唾液中的CgA样免疫反应性（CgA-like IR）。在3 μM苯肾上腺素（一种α-肾上腺素能激动剂）刺激下，约1 mM的CgA样IR分泌到唾液中。在Ca^<2+>缺乏的环境中，分泌反应几乎没有受到抑制，并且几乎完全被100 μM 2-氨基乙氧二苯硼酸盐（2-APB）抑制。这些结果表明，IP_3敏感池中Ca^<2+>的动员可能优先参与α-肾上腺素能激动剂的分泌反应。分泌性反应 ...更多信息对β-肾上腺素能激动剂异丙肾上腺素的反应在Ca^<2+>缺乏的环境中几乎保持不变，100μM 2-APB使反应明显延迟，但在去除2-APB和激动剂后逐渐恢复。Ca^2+从池中的动员也可能参与促进β-肾上腺素能反应中的协同信号传导。获得的结果表明，CgA样IR分泌到唾液中可能是交感神经活性，特别是α-肾上腺素能活性的指标，并提示大鼠下颌下腺中颗粒管细胞的肾上腺素能反应中可能的细胞内信号传导机制。牙科医院）。在牙科治疗如牙瘢痕形成期间对全唾液进行取样，然后使用试剂盒进行CgA测定。平均CgA为6.53 pmol/mg蛋白。CgA值在处理期间变化。而CgA值、血压、脉搏与精神应激程度无明显关系。少