Evaluation of mental stresses during dental treatments by chromogranin A in saliva

嗜铬粒蛋白A （CgA）与儿茶酚胺共同释放，是交感神经系统肾上腺素神经元的介质。本研究的目的是建立口腔治疗过程中唾液CgA的测量方法，并评价CgA与金属应力的关系。由Yanaihara研究所（Yanaihara Institute）开发的大鼠CgA酶免疫分析法（EIA），用于评估离体血管灌注大鼠颌下腺唾液管分泌唾液中的CgA样免疫活性（CgA样IR）。大约1mm的cga样IR在3 μM的苯肾上腺素（α-肾上腺素能激动剂）刺激下分泌到唾液中。Ca^<2+>-缺乏环境下，其分泌反应几乎没有受到抑制，而100 μM的2-氨基乙氧基二苯硼酸盐（2- apb）几乎完全抑制了分泌反应。这些结果表明，从IP_3敏感池中动员Ca^<2+>可能优先参与α-肾上腺素激动剂的分泌反应。在缺乏Ca^<2+>的环境中，β-肾上腺素能激动剂异丙肾上腺素的分泌反应基本保持不变，100μM - 2- apb明显延迟，但在去除2- apb和激动剂后逐渐恢复。从池中动员Ca^<2+>也可能参与促进β-肾上腺素能反应中的协同信号。结果表明，唾液分泌cga样IR可能是交感神经活性，尤其是α-肾上腺素能活性的一个指标，提示大鼠颌下腺颗粒管细胞肾上腺素能反应的胞内信号传导机制。对6名健康成人进行唾液CgA测定（北海道大学牙科医院）。在牙齿治疗（如牙齿惊吓）期间采集全唾液，然后使用试剂盒测定CgA。平均CgA为6.53 pmol/mg蛋白质。不同处理的CgA值不同。但CgA值、血压、脉搏与精神应激程度之间关系不明显。少

Chromogranin A (CgA) is co-released with catecholamines, mediators in adrenargic neurons of sympathetic nervous system. The purpose of the study is to establish the measurement of salivary CgA and to evaluate the relationship between CgA and metal stresses during dental treatments.Enzyme immunoassay (EIA) for rat CgA was developed (Yanaihara Institute), and used to estimate CgA-like immonoreactivity (CgA-like IR) in saliva secreted from the salivary duct of isolated vascularly perfused rat submandibular gland. Approxirnately 1 mM of CgA-like IR is secreted into saliva in response to stimulation with 3 μM phenylephrine, an α-adrenergic agonist. The secretory responses were scarcely inhibited in Ca^<2+>-deficient environment, and were almost completely inhibited by 100 μM 2-aminoethoxydiphenyl borate (2-APB). These results indicate that mobilization of Ca^<2+> from the IP_3 sensitive pool may preferentially be involved in the secretory responses to α-adrenargic agonist. The secretory res … More ponses to isoproterenol, β-adrenergic agonist, remained almost unchanged in the Ca^<2+>-deficient environment, and were markedly delayed by 100μM 2-APB but were regained gradually after removal of 2-APB and the agonist. Mobilization of Ca^<2+> from the pool may also be involved to facilitate synergistic signallings in the β-adrenergic responses. Results obtained show that CgA-like IR secretion into saliva may be an index of activity of the sympathetic nerve, especially α-adrenergic activity, and suggest a possible intracellular signalling mechanism in the adrenergic responses of the granulated duct cells in rat submandibular glands.In six healthy adults, salivary CgA was determined (Hokkaido Univ., Dental Hospital). Whole saliva was sampled during dental treatments such as dental scaring, then subjected to determine CgA using the kit. The mean CgA was 6.53 pmol/mg protein. The CgA value was varied during treatments. But there was no clear relation between CgA values, blood pressure, pulse and degree of mental stress. Less