Estimation of age from bone using the racemization of aspartic acid

研究了用天冬氨酸消旋化法测定骨组织的实足年龄。男性骨组织中SP分数与外消旋化程度的相关性高于TAA分数和IC分数，且存在性别差异。外消旋化程度与实足年龄的相关系数依次为股骨≧头盖骨≧胸骨bb0腰椎bb1髋骨bb2肋软骨bb3骶骨。外消旋化反应在牙本质中的反应速率比在骨中的反应速率低。4 .促进和延缓衰老模型小鼠颅骨外消旋化程度与衰老相关的性别差异大于与体温相关的性别差异。水解反应温度对外消旋化程度的影响最大的是试样的粒径和体积、水解反应的持续时间和温度、盐酸的体积和浓度。Glu和Ala与Asp结合使用的结果表明，该组合将有助于测试估计的实足年龄的可信度

Estimatin of chronological age using the aspartic acid racemization method was studied in the bone.1. Correlation between the chronological age and the degree of racemization in the SP fraction was higher than in the TAA or IC fraction of the male bone, and showed a sexual difference.2. Correlation coefficients between the degree of racemization and the chronological age were ranked in the order of femur≧cranium≧sternum>lumbar vertebra>coxal bone>costal cartilage>sacrum.3. Racemization reaction rate was lower in the bone than in the dentin.4. The degree of racemization in the cranium of the promoting and retarding strains of the senescence-accelerated model mouse showed a larger sexual difference in correlation with aging than with body temperature.5. The degree of racemization was most affected by the hydrolytic reaction temperature among the size of particles and volume of the specimen, the duration and temperature of hydrolytic reaction, and the volume and concentration of hydrochloric acid.6. The results of the use of Glu and Ala in combination with Asp suggested that the combination would be useful for testing the credibility of the estimated chronological age