Construction of lipid bilayer membrane on ion-exchange resin surface and the application as separation material

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In order to develop noble functional materials having properties of biomembrane, we have investigated the construction of lipid bilayer membrane on ionexchange resins. In the present study, the anion-exchange resin-DCP complex was prepared and characterized using dihexadecyl phospate (DCP) that is known to form the liposome as foolows :(1) The complex was prepared by short-time mixing of the wet ion-exchange resin with DCP-liposome solution. The results of FT-IR and X-ray fluorescence analysys indicated that the DCP was loaded on the resin and it was stable in the buffer solution. The determination of P in the complex revealed that the amount of DCP loaded on the resin was above 0.3 mmol/grsin that was higer P content than the caluculated value.(2) The DCP loading did not change the properties of the ion-excange resin matrix for inorganic anion, whereas in HPLC analysis of protein, the anion-excange resin matrix was shown to have the properties as the cation-exchange resin by DCP loading.(3) The DCP liposome was labeled with octadecyl rhodamineB (ODR). The observaton using laser scanning confocal microscope gave the fluoresence imaging that indicated the DCP layer was formed on the anionic-exchange resin.