Construction of lipid bilayer membrane on ion-exchange resin surface and the application as separation material

离子交换树脂表面脂质双层膜的构建及其作为分离材料的应用

• 批准号: 11672146
• 负责人: NAKAYAMA Morio
• 金额: $ 2.3万
• 依托单位: Nagasaki University (2000)Kumamoto University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672146/
• 关键词: bilayer membrane; ion-exhage resin; liposome; HPLC; laser scanning confocal microscope

In order to develop noble functional materials having properties of biomembrane, we have investigated the construction of lipid bilayer membrane on ionexchange resins. In the present study, the anion-exchange resin-DCP complex was prepared and characterized using dihexadecyl phospate (DCP) that is known to form the liposome as foolows :(1) The complex was prepared by short-time mixing of the wet ion-exchange resin with DCP-liposome solution. The results of FT-IR and X-ray fluorescence analysys indicated that the DCP was loaded on the resin and it was stable in the buffer solution. The determination of P in the complex revealed that the amount of DCP loaded on the resin was above 0.3 mmol/grsin that was higer P content than the caluculated value.(2) The DCP loading did not change the properties of the ion-excange resin matrix for inorganic anion, whereas in HPLC analysis of protein, the anion-excange resin matrix was shown to have the properties as the cation-exchange resin by DCP loading.(3) The DCP liposome was labeled with octadecyl rhodamineB (ODR). The observaton using laser scanning confocal microscope gave the fluoresence imaging that indicated the DCP layer was formed on the anionic-exchange resin.

为了开发具有生物膜特性的高性能功能材料，我们研究了在离子交换树脂上构筑脂双层膜。本研究以磷酸双十六烷基酯（DCP）为脂质体的载体，制备了阴离子交换树脂-DCP复合物，并对其进行了表征：（1）将湿离子交换树脂与DCP-脂质体溶液短时混合制备复合物。FT-IR和X射线荧光分析结果表明，DCP负载在树脂上，在缓冲溶液中稳定。络合物中磷的测定表明，DCP负载量大于0.3mmol/g，高于理论计算值。(2)DCP负载量不改变离子交换树脂基质对无机阴离子的性质，而在蛋白质的HPLC分析中，阴离子交换树脂基质被证明具有通过DCP负载的阳离子交换树脂的性质。(3)用十八烷基罗丹明B（ODR）标记DCP脂质体。激光扫描共聚焦显微镜观察得到的荧光图像表明DCP层在阴离子交换树脂上形成。