Construction of lipid bilayer membrane on ion-exchange resin surface and the application as separation material

离子交换树脂表面脂质双层膜的构建及其作为分离材料的应用

• 批准号: 11672146
• 负责人: NAKAYAMA Morio
• 金额: $ 2.3万
• 依托单位: Nagasaki University (2000)Kumamoto University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672146/
• 关键词: bilayer membrane; ion-exhage resin; liposome; HPLC; laser scanning confocal microscope

In order to develop noble functional materials having properties of biomembrane, we have investigated the construction of lipid bilayer membrane on ionexchange resins. In the present study, the anion-exchange resin-DCP complex was prepared and characterized using dihexadecyl phospate (DCP) that is known to form the liposome as foolows :(1) The complex was prepared by short-time mixing of the wet ion-exchange resin with DCP-liposome solution. The results of FT-IR and X-ray fluorescence analysys indicated that the DCP was loaded on the resin and it was stable in the buffer solution. The determination of P in the complex revealed that the amount of DCP loaded on the resin was above 0.3 mmol/grsin that was higer P content than the caluculated value.(2) The DCP loading did not change the properties of the ion-excange resin matrix for inorganic anion, whereas in HPLC analysis of protein, the anion-excange resin matrix was shown to have the properties as the cation-exchange resin by DCP loading.(3) The DCP liposome was labeled with octadecyl rhodamineB (ODR). The observaton using laser scanning confocal microscope gave the fluoresence imaging that indicated the DCP layer was formed on the anionic-exchange resin.

为了开发具有生物膜性能的贵金属功能材料，我们在离子交换树脂上构建了脂类双层膜。在本研究中，我们制备了阴离子交换树脂-DCP络合物，并对其进行了表征：(1)将阴离子交换树脂与DCP-脂质体溶液进行短时混合，制得阴离子交换树脂-DCP络合物。FT-IR和X-射线荧光分析结果表明，DCP负载在树脂上，在缓冲溶液中稳定。络合物中磷的测定表明，DCP在树脂上的负载量在0.3 mmol/g以上，P含量高于计算值。(2)DCP的负载没有改变离子交换树脂基质对无机阴离子的性质，而在蛋白质的高效液相分析中，负载DCP的阴离子交换树脂基质具有阳离子交换树脂的性质。(3)用十八烷基罗丹明B(ODR)标记了DCP脂质体。激光扫描共聚焦显微镜观察到的荧光图像表明，DCP层已在阴离子交换树脂表面形成。