Development and Application of chiral separation HPLC methods with fluorescence or chemiluminescence detection for determination of metamphetamines

荧光或化学发光检测手性分离高效液相色谱法测定甲基苯丙胺的开发及应用

• 批准号: 11672145
• 负责人: NAKASHIMA Kenichiro
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672145/
• 关键词: Abuse drugs; methamphetamines; fluorescence detection; high-performance liquid chromatography; chiral separation; semi-micro column HPLC; chemiluminescence detection; ロフィン誘導体

A highly sensitive HPLC method for the determination of methamphetamines (MPs) has been developed using a newly synthesized fluorescent reagent, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. DIB derivatives of MPs, e.g., metamphetamine, amphetamine, p-hydroxymethamphetamine, and ephedrine, were separated and detected with peroxyoxalate chemiluminescence (PO-CL) and fluorescence methods. Fluorescence detection was more sensitive than PO-CL, thus HPLC with fluorescence detection was selected in this study. The developed method could detect methamphetamines at amol〜fmol levels. Some abusers' urine and hair samples were successfully determined by the method. We also focused ourselves to develop simple and economic methods by downsizing the system using semi-micro column HPLC.Achiral and chiral semi-micro column HPLC methods have been developed, which could be successfully applied to the determination of MPs in only 10 μL of urine or 1 cm-long hair samples.The proposed methods are simple and sensitive, and thus, they would be useful for pharmacological, toxicological and pharmaceutical studies, especially for the studies with samples including trace amounts of MPs.

以新合成的荧光试剂4-（4，5-二苯基-1H-咪唑-2-基）苯甲酰氯（DIB-Cl）为标记物，建立了一种高灵敏度的甲基苯丙胺（MP）的HPLC测定方法。MP的DIB衍生物，用过氧化草酸盐化学发光法和荧光法对甲基苯丙胺、苯丙胺、对羟甲基苯丙胺和麻黄碱进行了分离和检测。荧光检测法比PO-CL法灵敏，因此本研究选择了荧光检测的高效液相色谱法。所开发的方法可以检测到甲基苯丙胺在阿莫尔fmol水平。用该方法成功地测定了部分吸毒者的尿样和头发样品。本研究还建立了非手性和手性半微柱高效液相色谱法，可用于10 μL尿样和1cm头发样品中MP的测定，方法简便、灵敏，为药理学、毒理学和药物学研究提供了新的方法。特别是对于包括痕量MP的样品的研究。

项目成果

O.Al-Dirbashi,et al.: "HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair"Analyst. 124. 493-497 (1999)

O.Al-Dirbashi 等人：“HPLC 荧光检测分段分析的人发中的甲基苯丙胺和苯丙胺”分析师。

O.Al-Dirbashi, M.Wada, N.Kuroda, S.Inuzuka, K.Nakashima: "Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine"Biomedical Chromatography. 13. 543-547

O.Al-Dirbashi、M.Wada、N.Kuroda、S.Inuzuka、K.Nakashima：“对映体特异性高效液相色谱法，荧光检测滥用者头发和尿液中的甲基苯丙胺”生物医学色谱法。

中島憲一郎: "化学発光計測を利用する分析法"BUNSEKI KAGAKU. 49(3). 135-159 (2000)

Kenichiro Nakajima：“使用化学发光测量的分析方法”BUNSEKI KAGAKU 49(3)(2000)。

O.Y.Al-Dirbashi, et al.: "Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid....."Journal of Forensic Sciences. 45(3). 708-714 (2000)

O.Y.Al-Dirbashi 等人：“通过半微柱高性能液体对人尿液中的甲基苯丙胺和苯丙胺进行非手性和手性定量......”法医学杂志。

O.Al-Dirbashi, et al.: "Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamine......"Biomedical Chromatography. 13. 543-547 (1999)

O.Al-Dirbashi 等人：“对映体特异性高效液相色谱法与荧光检测甲基苯丙胺……”生物医学色谱法。