Development and Application of chiral separation HPLC methods with fluorescence or chemiluminescence detection for determination of metamphetamines

荧光或化学发光检测手性分离高效液相色谱法测定甲基苯丙胺的开发及应用

• 批准号: 11672145
• 负责人: NAKASHIMA Kenichiro
• 金额: $ 2.24万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11672145/
• 关键词: Abuse drugs; methamphetamines; fluorescence detection; high-performance liquid chromatography; chiral separation; semi-micro column HPLC; chemiluminescence detection; ロフィン誘導体

A highly sensitive HPLC method for the determination of methamphetamines (MPs) has been developed using a newly synthesized fluorescent reagent, 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. DIB derivatives of MPs, e.g., metamphetamine, amphetamine, p-hydroxymethamphetamine, and ephedrine, were separated and detected with peroxyoxalate chemiluminescence (PO-CL) and fluorescence methods. Fluorescence detection was more sensitive than PO-CL, thus HPLC with fluorescence detection was selected in this study. The developed method could detect methamphetamines at amol〜fmol levels. Some abusers' urine and hair samples were successfully determined by the method. We also focused ourselves to develop simple and economic methods by downsizing the system using semi-micro column HPLC.Achiral and chiral semi-micro column HPLC methods have been developed, which could be successfully applied to the determination of MPs in only 10 μL of urine or 1 cm-long hair samples.The proposed methods are simple and sensitive, and thus, they would be useful for pharmacological, toxicological and pharmaceutical studies, especially for the studies with samples including trace amounts of MPs.

使用新合成的荧光试剂，4-（4,5-二苯基-1H- imidazol-2-基）苯甲酰氯化物（DIB-CL）作为标签，一种使用新合成的荧光试剂（4,5-二苯基-1H-咪唑-2-基）作为标签开发了一种高度敏感的HPLC方法。将MPS的DIB衍生物（例如甲基苯丙胺，苯丙胺，p-羟基甲基苯丙胺和麻黄碱）分离，并用过氧氧化化学发光（PO-CL）和荧光方法分离并检测到。荧光检测比PO-CL更敏感，因此在本研究中选择了具有荧光检测的HPLC。开发的方法可以检测AMOL 〜FMOL水平的甲基苯丙胺。一些施虐者的尿液和头发样本成功地通过该方法确定。我们还开发了使用半微米列HPLC缩小系统来开发简单和经济的方法，已经开发出了手术和手性半微米列HPLC方法，这可以成功地应用于10μl尿液或1 cm长的毛发样本中的MPS的确定。特别是对于使用样品在内的研究，包括痕量的MPS。

项目成果

O.Al-Dirbashi,et al.: "HPLC with fluorescence detection of methamphetamine and amphetamine in segmentally analyzed human hair"Analyst. 124. 493-497 (1999)

O.Al-Dirbashi 等人：“HPLC 荧光检测分段分析的人发中的甲基苯丙胺和苯丙胺”分析师。

中島憲一郎: "化学発光計測を利用する分析法"BUNSEKI KAGAKU. 49(3). 135-159 (2000)

Kenichiro Nakajima：“使用化学发光测量的分析方法”BUNSEKI KAGAKU 49(3)(2000)。

O.Al-Dirbashi, M.Wada, N.Kuroda, S.Inuzuka, K.Nakashima: "Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamines in abusers' hair and urine"Biomedical Chromatography. 13. 543-547

O.Al-Dirbashi、M.Wada、N.Kuroda、S.Inuzuka、K.Nakashima：“对映体特异性高效液相色谱法，荧光检测滥用者头发和尿液中的甲基苯丙胺”生物医学色谱法。

O.Y.Al-Dirbashi, et al.: "Achiral and chiral quantification of methamphetamine and amphetamine in human urine by semi-micro column high-performance liquid....."Journal of Forensic Sciences. 45(3). 708-714 (2000)

O.Y.Al-Dirbashi 等人：“通过半微柱高性能液体对人尿液中的甲基苯丙胺和苯丙胺进行非手性和手性定量......”法医学杂志。

O.Al-Dirbashi, et al.: "Enantiomer-specific high-performance liquid chromatography with fluorescence detection of methamphetamine......"Biomedical Chromatography. 13. 543-547 (1999)

O.Al-Dirbashi 等人：“对映体特异性高效液相色谱法与荧光检测甲基苯丙胺……”生物医学色谱法。