Molecular Pharmacological Studies on Effect of Docosahexaenoic Acid on Vascular Cell Functions
二十二碳六烯酸对血管细胞功能影响的分子药理学研究
基本信息
- 批准号:11672177
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1999
- 资助国家:日本
- 起止时间:1999 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The aim of the present project was to investigate the effects of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), n-3 polyunsaturated fatty acids, on protein and mRNA expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in cultured vascular smooth muscle cells isolated from 6-7 week-old stroke-prone spontaneously hypertensive rats (SHRSP) and age-matched normotensive Wistar Kyoto rats (WKY). The IL-1β-induced nitric oxide (NO) production and iNOS expression in cells of SHRSP were significantly lower than those in cells of WKY.Similarly, prostaglandins (PG)I2 production and COX-2 expression were significantly lower in cells of SHRSP than WKY, whereas there was no difference in the COX-1 expression. There were no significant differences in iNOS and COX-2 mRNA expressions between the two strains, suggesting that these protein expression may be impaired at post-transcriptional process in cells of SHRSP.DHA and EPA significantly enhanced the IL-1β-induced NO production in a concentration-dependent manner in cells of WKY, whereas had no effect in cells of SHRSP.DHA and EPA also significantly enhanced iNOS protein and mRNA expressions induced by IL-1β in cells of WKY but not in cells of SHRSP.DHA and EPA significantly increased COX-2, but not COX-1, protein expression induced by IL-1β in cells of WKY, but had no effect on COX-2 mRNA expression. These results suggest that the impairment of iNOS and COX-2 expressions in vascular smooth muscle cells may have relevance to the pathophysiology in SHRSP.These results further suggest that enhancement of NO and PGI2 productions by DHA may account for its beneficial effect on cardiovascular disorders.
本项目的目的是研究二十二碳六烯酸(DHA)和二十碳五烯酸(EPA),n-3多不饱和脂肪酸,诱导型一氧化氮合酶(iNOS)和环氧合酶-2(考克斯-2)在6-7周龄易卒中型自发性高血压大鼠(SHRSP)血管平滑肌细胞中的蛋白和mRNA表达。匹配的血压正常的Wistar京都大鼠(WKY)。SHRSP细胞在IL-1β诱导下的NO生成和iNOS表达均显著低于WKY细胞,且SHRSP细胞的前列腺素(PG)I2生成和考克斯-2表达均显著低于WKY细胞,而考克斯-1表达无显著差异。两株SHRSP细胞iNOS和考克斯-2 mRNA表达无明显差异,提示这些蛋白的表达可能在转录后过程中受到损伤。DHA和EPA可显著增强IL-1β诱导的WKY细胞NO生成,并呈浓度依赖性。DHA和EPA对IL-10诱导的SHRSP细胞iNOS蛋白和mRNA的表达也有明显的促进作用,DHA和EPA可显著增加IL-1β诱导的WKY细胞考克斯-2蛋白表达,但对考克斯-1蛋白表达无影响,对考克斯-2 mRNA表达无影响。上述结果提示,血管平滑肌细胞iNOS和考克斯-2表达的降低可能与SHRSP的病理生理有关,DHA促进NO和PGI 2的生成可能是其治疗心血管疾病的机制之一。
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
平藤雅彦: "ドコサヘキサエン酸はなぜ循環器疾患に有効なのか-血管…"道薬誌. 18(1). 30-35 (2001)
Masahiko Hirafuji:“为什么二十二碳六烯酸对心血管疾病有效?血管……”Doyakushu. 18(1)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
平藤雅彦: "腸クロム親和性細胞の細胞内カルシウム動態解析法"日本薬理学雑誌. 114(12). 357-363 (1999)
Masahiko Hirafuji:“分析肠嗜铬细胞内钙动力学的方法”,日本药理学杂志 114(12) 357-363 (1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hirafuji,M.: "Progress in Hypertension, Vol.4"Parveg,SH, Frossard, PM, Minami M, Parveg S and Sato H. 267 (1999)
Hirafuji,M.:“高血压进展,第 4 卷”Parveg,SH、Frossard, PM、Minami M、Parveg S 和 Sato H. 267 (1999)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hirafuji, M., Machida, T., Kawahara, A.and Minami, M.: "NO production and iNOS expression by vascular smooth muscle cells of normotensive and genetically hypertensive rats : effect of docosahexaenoic acid (DHA)."Jpn.J.Circ.Res.. 24 (2). 37-42 (2001)
Hirafuji, M.、Machida, T.、Kawahara, A. 和 Minami, M.:“正常血压和遗传性高血压大鼠血管平滑肌细胞的 NO 产生和 iNOS 表达:二十二碳六烯酸 (DHA) 的作用。”Jpn.J
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Hirafuji: "5-Hydorxytryptawine iuduces trausient Ca^<2+> influx through Ni^<2+>-insensitive Ca^<2+> channels in rat vaswlar smooth muscle cells"European Journal of Pharwacology. 380. 163-170 (1999)
M.Hirafuji:“5-羟色氨酸通过大鼠血管平滑肌细胞中的 Ni^<2>-不敏感 Ca^<2> 通道诱导创伤性 Ca^<2> 流入”欧洲药理学杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
HIRAFUJI Masahiko其他文献
HIRAFUJI Masahiko的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('HIRAFUJI Masahiko', 18)}}的其他基金
Studies on the role of nitric oxide in abnormal intestinal serotonin metabolism using a model rat of intestinal damage
利用肠道损伤模型大鼠研究一氧化氮在肠道血清素代谢异常中的作用
- 批准号:
22590085 - 财政年份:2010
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Effects of Docosahexaenoic Acids on Vascular Cell Functions
二十二碳六烯酸对血管细胞功能的影响
- 批准号:
09672244 - 财政年份:1997
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
STUDIES ON THE MECHANISM OF ENDOTOXIN-INDUCED MICROIRCULATORY DISTURBANCE IN DENTAL PULP TUSSUE
内毒素引起牙髓脓肿微循环障碍机制的研究
- 批准号:
04671121 - 财政年份:1992
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Roles of lipid mediators in the mechanism of dental pulp tissue injury induced by administration of pharmacological agents
脂质介质在药物致牙髓组织损伤机制中的作用
- 批准号:
02670825 - 财政年份:1990
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Pharmacological Study on the Possible Roles of Platelet-activating Factor (PAF) in Inflammatory Process of the Dental Pulp
血小板活化因子(PAF)在牙髓炎症过程中可能作用的药理学研究
- 批准号:
62570827 - 财政年份:1987
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Prebiotic effect of eicosapentaenoic acid treatment for colorectal cancer
二十碳五烯酸治疗结直肠癌的益生元作用
- 批准号:
10406256 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
Basic research on the effects of eicosapentaenoic acid metabolites on upper airway inflammation
二十碳五烯酸代谢物对上呼吸道炎症影响的基础研究
- 批准号:
20K09754 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Prebiotic effect of eicosapentaenoic acid treatment for colorectal cancer
二十碳五烯酸治疗结直肠癌的益生元作用
- 批准号:
10620849 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
Prebiotic effect of eicosapentaenoic acid treatment for colorectal cancer
二十碳五烯酸治疗结直肠癌的益生元作用
- 批准号:
10161752 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
Development of novel therapeutic method for diabetic nephropathy by using eicosapentaenoic acid
二十碳五烯酸治疗糖尿病肾病新方法的开发
- 批准号:
17K18257 - 财政年份:2017
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Eicosapentaenoic acid inhibits skin fibrosis in acquired lymphedema
二十碳五烯酸抑制获得性淋巴水肿中的皮肤纤维化
- 批准号:
17K17788 - 财政年份:2017
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Study of anti-obesity effect via eicosapentaenoic acid-induced improvement of skeletal muscle function
二十碳五烯酸改善骨骼肌功能的抗肥胖作用研究
- 批准号:
17K15272 - 财政年份:2017
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Effects of eicosapentaenoic acid on anticancer drug-induced weight loss
二十碳五烯酸对抗癌药物引起的减肥的影响
- 批准号:
16K21401 - 财政年份:2016
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Association of Improvement of HDL Metabolism and Eicosapentaenoic Acid with Prevention of Coronary Artery Disease
HDL 代谢和二十碳五烯酸的改善与预防冠状动脉疾病的关联
- 批准号:
16K09045 - 财政年份:2016
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The optimization of eicosapentaenoic acid and docosahexaenoic acid for breast cancer prevention
二十碳五烯酸和二十二碳六烯酸预防乳腺癌的优化
- 批准号:
367359 - 财政年份:2016
- 资助金额:
$ 2.18万 - 项目类别:
Studentship Programs