Study of Life Science as Complex Systems

我们已经建立了复杂系统生物学的基础，通过采取建设性的方法，在实验和理论上建立一个简单的系统，揭示所有生命系统的普遍特征。(1)由DNA和DNA聚合酶等组成的体外人工复制体系的合成。理论证明，数量较少的分子开始发挥遗传的作用，从某种意义上说，它们控制细胞的行为相对强烈，并保存得很好。上述实验证实了这一理论。(2)脂质体中蛋白质合成和RNA复制的成功，脂质体稳定自我复制的观察和构建，脂质体DNA杂交。(3)理论发现维持递归生产的细胞中化学丰度的普遍统计规律，即平均基因表达的幂律和每种化学物质丰度的对数正态分布，如实验证实的那样。(4)利用激活素和其他一些蛋白质，几乎所有器官都可以从异种未分化的细胞中生成。跳跃现象和群体效应的发现。证明所构建的眼睛和心脏组织在移植到生物体后能正常工作。(5)细胞分化发育过程理论基于一个耦合的动力系统，显示出鲁棒性和不可逆性。(6)利用嵌入基因网络构建大肠杆菌分化。(7)大肠杆菌的进化，表明在拥挤条件下诱导多样性。(8)共生同域物种形成理论的提出。(9)大肠杆菌与阿米巴原虫共生系统的构建。（10）进化速度与克隆表型波动成正比的理论命题，大肠杆菌蛋白荧光增强的人工选择实验证实了这一点。（11）开发一种设备系统，允许长期单细胞观察、操作和片上细胞分选。少

We have set up the basis of complex systems biology, to unveil universal features underlying all life systems, by taking a constructive approach, to set up a simple system both experimentally and theoretically.(1)Synthesis of in-vitro artificial replication system consisting of DNA and DNA polymerase and others. Theoretical demonstration that molecules minority in number start to play the role of heredity, in the sense that they control the behavior of a cell relatively strongly and are preserved well. This theory is confirmed by the above experiment.(2)Success of protein synthesis and RNA replication in liposome, observation and construction of stable self-replication of liposomes, and DNA hybridization on liposome.(3)Theoretical discovery of universal statistical laws of chemical abundances in a cell that sustains recursive production, i.e., a power law in average gene expression and log-normal distribution of the abundances of each chemicals, as is confirmed experimentally.(4)In-vit … More ro genesis of almost all organs from Xenopous undifferentiated cells, using activin and a few other proteins. Discovery of jump-over phenomena and community effect. Demonstration that the constructed eye and heart tissues work normally after transplantation to organisms.(5)Theory of cell differentiation and developmental process based on a coupled dynamical systems, to show robustness and irreversibility.(6)Construction of differentiation by E Coli by using embedded gene network.(7)Evolution of E Coli to show that diversity is induced in a crowded condition.(8)Proposition of a theory of symbiotic sympatric speciation.(9)Construction of symbiotic system by E Coli and amoeba.(10)Theoretical proposition that evolution speed is proportional to phenotypic fluctuation of clones, as confirmed by artificial selection experiment to increase fluorescence of protein in E Coli.(11)Development of an equipment system that allows for long-term singe cell observation, manipulation, and on-chip cell sorter. Less