Study of Life Science as Complex Systems

生命科学作为复杂系统的研究

• 批准号: 11CE2006
• 负责人: KANEKO Kunihiko
• 金额: $ 1075.84万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for COE Research
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11CE2006/
• 关键词: Constructive Biology; Self-replication system; cell differentiation; Activin; memory; micro-fabrication; sympatric speciation; symbiosis; 発生; 分化; 進化; 複雑系; 力学系; 細胞質記憶; 人工細胞システム

We have set up the basis of complex systems biology, to unveil universal features underlying all life systems, by taking a constructive approach, to set up a simple system both experimentally and theoretically.(1)Synthesis of in-vitro artificial replication system consisting of DNA and DNA polymerase and others. Theoretical demonstration that molecules minority in number start to play the role of heredity, in the sense that they control the behavior of a cell relatively strongly and are preserved well. This theory is confirmed by the above experiment.(2)Success of protein synthesis and RNA replication in liposome, observation and construction of stable self-replication of liposomes, and DNA hybridization on liposome.(3)Theoretical discovery of universal statistical laws of chemical abundances in a cell that sustains recursive production, i.e., a power law in average gene expression and log-normal distribution of the abundances of each chemicals, as is confirmed experimentally.(4)In-vit … More ro genesis of almost all organs from Xenopous undifferentiated cells, using activin and a few other proteins. Discovery of jump-over phenomena and community effect. Demonstration that the constructed eye and heart tissues work normally after transplantation to organisms.(5)Theory of cell differentiation and developmental process based on a coupled dynamical systems, to show robustness and irreversibility.(6)Construction of differentiation by E Coli by using embedded gene network.(7)Evolution of E Coli to show that diversity is induced in a crowded condition.(8)Proposition of a theory of symbiotic sympatric speciation.(9)Construction of symbiotic system by E Coli and amoeba.(10)Theoretical proposition that evolution speed is proportional to phenotypic fluctuation of clones, as confirmed by artificial selection experiment to increase fluorescence of protein in E Coli.(11)Development of an equipment system that allows for long-term singe cell observation, manipulation, and on-chip cell sorter. Less

我们已经建立了复杂系统生物学的基础，通过采取建设性的方法，揭示所有生命系统的普遍特征，建立一个简单的系统，实验和理论。（1）由DNA和DNA聚合酶等组成的体外人工复制系统的合成。理论证明，分子少数在数量开始发挥遗传的作用，在这个意义上说，他们控制的行为细胞相对较强，并保存良好。上述实验证实了这一理论。（2）脂质体中蛋白质合成和RNA复制的成功，脂质体稳定自我复制的观察和构建，脂质体上DNA杂交。（3）从理论上发现维持递归生产的细胞中化学丰度的普遍统计规律，即，平均基因表达的幂律和每种化学物质丰度的对数正态分布，如实验所证实的。（4）体内 ...更多信息 利用激活素和一些其他蛋白质，从异种未分化细胞中再生几乎所有器官。跳跃现象与群体效应的发现。证明构建的眼睛和心脏组织移植到生物体后正常工作。（5）基于耦合动力系统的细胞分化和发育过程理论，以显示鲁棒性和不可逆性。（6）利用包埋基因网络构建大肠杆菌分化株。（7）大肠杆菌的进化，表明在拥挤的条件下诱导多样性。（8）共生共域物种形成理论的提出。（9）大肠杆菌与阿米巴共生体系的构建。（10）进化速度与克隆表型波动成正比的理论命题，通过人工选择实验证实，以增加大肠杆菌中蛋白质的荧光。（11）开发允许长期单细胞观察、操作和芯片上细胞分选器的设备系统。少

项目成果

Wada, M., Miyazawa, H., Wang, R., Mizuno, T., Sato, A., Asashima, M., Hanaoka, F.: "The second largest subunit of mouse DNA polymerase ε, DPE2, interacts with SASP18 and recruits the Sin3 co-repressor protein to DNA"J.Biochem.. 131. 307-311 (2002)

Wada, M.、Miyazawa, H.、Wang, R.、Mizuno, T.、Sato, A.、Asashima, M.、Hanaoka, F.：“小鼠 DNA 聚合酶 ε 的第二大亚基 DPE2 与SASP18 并将 Sin3 辅阻遏蛋白招募至 DNA”J.Biochem.. 131. 307-311 (2002)

K.Kaneko: "Recursiveness and Evolvability in Mutually Catalytic Reaction System"Advances in Complex Systems. (in press). (2003)

K.Kaneko：“互催化反应系统中的递归性和演化性”复杂系统的进展。

K.Kaneko, T.Yomo: "Genetic Diversification through Interaction-driven Phenotype Differentiation"Evol Eco.Res.. 4. 317-350 (2003)

K.Kaneko、T.Yomo：“通过相互作用驱动的表型分化实现遗传多样性”Evol Eco.Res.. 4. 317-350 (2003)

K.Kaneko: "Dominance of Minlnor attractors in Globally Coupled Dynamical Systems with more than 7 + -2 degrees of freedom"Phys.Rev.E.. 66. 055201(R) (2002)

K.Kaneko：“自由度超过 7 -2 的全局耦合动力系统中次小吸引子的支配地位”Phys.Rev.E.. 66. 055201(R) (2002)

S.Ishihara, K.Kaneko: "Simple dynamical system model of history dependent phenomena"J.Phys.Soc.Jpn.. 71,10. 2357-2360 (2002)

S.Ishihara、K.Kaneko：“历史相关现象的简单动力系统模型”J.Phys.Soc.Jpn.. 71,10。