Function of membrane skeletal protein (protein 4.1) in osteoblast for cell adhesion and secretion of local factor

成骨细胞膜骨骼蛋白（蛋白4.1）对细胞粘附和局部因子分泌的作用

• 批准号: 12671443
• 负责人: HORIKOSHI M
• 金额: $ 2.56万
• 依托单位: TOKYO WOMEN'S MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671443/
• 关键词: Osteoblast; protein 4.1R; protein 4.1G; CD44; Type I collagen; cell adhesion; Transfection; Laser Confocal Microscopy; 膜骨格蛋白; 4.1蛋白質; ノックアウトマウス

Remodeling of bone tissue is maintained by bone formation and absorption which are regulated by osteoblasts and osteoclasts. In regulation of bone formation, multiple osteoblast surface receptors mediate interactions with the surrounding extracellular matrix and thereby influence cell adhesion. The adhesiont of osteoblast CD44 and type I collagen in extracellular matrix is the important event of bone formation. We tried to investigate the reguration of the osteoblast CD44 and type 1 collagen adhesion. Immune fluorescence staining and cell adhesion test on type I collagen coated dishes showed the same location of CD44 and protein 4.1R in osteoblast analyzed by the LASER Confocal Microscopy. The transfection of protein 4.1R gene for osteoblasts emphasized the CD44 and type I collagen adhesion. Protein 4.1G also was concerned in the interaction of CD44 and type I collagen by the result of RT-PCR.These results suggest that the protein 4.1R and 4.1G may be involved in the regulation of CD44 and type I collagen adhesion and the osteoblast function in bone formation.

骨组织的重塑是由成骨细胞和破骨细胞调节的骨形成和吸收来维持的。在骨形成的调控中，多种成骨细胞表面受体介导与周围细胞外基质的相互作用，从而影响细胞粘附。细胞外基质中成骨细胞CD44与I型胶原的黏附是骨形成的重要事件。我们试图研究成骨细胞CD44和1型胶原黏附的调节。免疫荧光染色和I型胶原包被培养皿细胞粘附试验显示，激光共聚焦显微镜分析CD44和4.1R蛋白在成骨细胞中的位置相同。转染蛋白4.1R基因后，成骨细胞对CD44和I型胶原的粘附增强。RT-PCR结果显示，4.1G蛋白也参与了CD44与I型胶原的相互作用。这些结果提示，4.1R和4.1G蛋白可能参与了骨形成过程中CD44和I型胶原黏附及成骨细胞功能的调控。