Evaluation method development of endocrine disruptor effect and pollution analysis by its application

内分泌干​​扰物效应评价方法的建立及应用污染分析

基本信息

  • 批准号:
    11793003
  • 负责人:
  • 金额:
    $ 0.64万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for University and Society Collaboration
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2001
  • 项目状态:
    已结题

项目摘要

Some kinds of devices have been developed to determine specific chemicals electrochemically after immunoreactions. In the one of the devices, a SPR cell was used as a reaction field. Immobilization of antibody and immunoreactions were conducted with monitoring with the SPR signal and final product by enzyme reactions were detected electrochemically. This method had an advantage that the complicated procedure of sandwich immunoassay process could be monitored and could be successfully continued to the final electrochemical enzyme assay. The antigen could be measured by the SPR signal only when the concentration was high, and the electrochemical determination had 1000 time greater sensitivity than the SPR's. A reaction field was fabricated on a gold film by the process establish with the help of the SPR. Usually, the immobilization needs long time such as one night, when the antibody was conuected on a glass substrate with glutalaldehyde. On the contrary, the antibody could be immobilized on the gold film in a short time. When we used SPR instrument, the immobilization area was limited. But, by using the large immobilization area, the enzyme reaction could be done without the flow stop for the incubation. The other device consisted of a micro-capillary. With this capillary device, only 5 μL of sample was needed for the determination. The detection limit of IgG was very small, 2 amol. This device was expected to be used with inserting into a living body directly.PCBs contained in living things and sediments were investigated by means of GC/MS. It was found that the PCB level at the bottom was higher than the mouth of the bay. PCBs levels in many samples of B. pectinirostris were measured. The concentration was related to the body length rather than to their age.
已经开发了一些类型的装置,用于在免疫反应后电化学测定特定的化学物质。在其中一个装置中,SPR池被用作反应场。通过SPR信号监测抗体的固定和免疫反应,并通过电化学检测酶反应的最终产物。该方法的优点是可以监测夹心免疫分析过程的复杂步骤,并可以成功地持续到最终的电化学酶分析。只有当抗原浓度较高时,SPR信号才能检测到抗原,电化学检测的灵敏度比SPR高1000倍。利用表面等离子体共振技术在金膜上制作了反应场。通常,固定化需要较长的时间,如一个晚上,当抗体与戊二醛连接在玻璃基板上时。相反,抗体可以在短时间内固定在金膜上。使用SPR仪器时,固定化面积有限。但是,通过使用大的固定化面积,可以在没有用于温育的流动停止的情况下进行酶反应。另一个装置由微毛细管组成。该毛细管装置只需5 μL样品即可测定。IgG的检出限很小,为2阿莫尔。采用GC/MS法对生物和沉积物中的PCB含量进行了分析,结果表明,底质中的PCB含量高于湾口。B的许多样品中的多氯联苯水平。测量了大胸鳍鱼。浓度与体长有关,与年龄无关。

项目成果

期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Toda: "Fluorometric field instrument for continuous measurement of atmospheric hydrogen sulfide"Analytical Chemistry. 73. 5716-5724 (2001)
K.Toda:“连续测量大气硫化氢的荧光现场仪器”分析化学。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
戸田 敬: "液的マイクロリング電極による電気化学的ガス計測方法の検討"分析化学. 49. 989-995 (2000)
Takashi Toda:“使用液体微环电极的电化学气体测量方法的研究”分析化学49。989-995(2000)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
中田晴彦: "有明海の干潟底質におけるPCBs濃度分布とその残留特性"環境化学. 12(in press). (2002)
Haruhiko Nakata:“有明海潮滩沉积物中的 PCB 浓度分布和残留特征”环境化学 12(出版中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K. Toda, S. Oguni, Y. Takamatsu and I. Sanemasa: "A wall-jet ring disk electrode fabricated within a thin-layered micromachined cell"Journal of Electroanalytical Chemistry. 479. 57-63 (1999)
K. Toda、S. Oguni、Y. Takamatsu 和 I. Sanemasa:“在薄层微加工电池内制造的壁射流环盘电极”电分析化学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K. Toda, M. Kitazaki and I. Sanemasa: "Amperometric gas detection using a micro-ring electrode coupled with a gas-absorbing droplet"Bunsekl Kagaku. 49. 989-995 (2000)
K. Toda、M. Kitazaki 和 I. Sanemasa:“使用微环电极与气体吸收液滴耦合的电流分析气体检测”Bunsekl Kagaku。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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TODA Kei其他文献

Universal Detection Methods in Ion Chromatography
离子色谱中的通用检测方法
  • DOI:
    10.2116/bunsekikagaku.68.153
  • 发表时间:
    2019
  • 期刊:
  • 影响因子:
    0.2
  • 作者:
    OHIRA Shin-Ichi;TODA Kei
  • 通讯作者:
    TODA Kei
Electrodialytic Matrices Isolation for Determination of Heavy Metals in Soil Extracts by Anodic Stripping Voltammetry
阳极溶出伏安法测定土壤提取物中重金属的电渗析基质分离
  • DOI:
    10.2116/bunsekikagaku.67.761
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0.2
  • 作者:
    OHIRA Shin-Ichi;OKAZAKI Chinatsu;TODA Kei
  • 通讯作者:
    TODA Kei

TODA Kei的其他文献

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{{ truncateString('TODA Kei', 18)}}的其他基金

Micro gas analysis system comprising of air/liquid direct contact andits applications to environmental analysis and medical diagnostics
空气/液体直接接触微量气体分析系统及其在环境分析和医疗诊断中的应用
  • 批准号:
    21550087
  • 财政年份:
    2009
  • 资助金额:
    $ 0.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Multi-Gas Analysis of Atmospheric Sulfur Gases by Means of Microscrubber and its Application to Dynamics Analysis at Tidal Flat
微洗涤器对大气含硫气体的多气体分析及其在滩涂动力学分析中的应用
  • 批准号:
    15550075
  • 财政年份:
    2003
  • 资助金额:
    $ 0.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Micro gas analytical system by means of radial electrophoresis pump
采用径向电泳泵的微量气体分析系统
  • 批准号:
    11640611
  • 财政年份:
    1999
  • 资助金额:
    $ 0.64万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似国自然基金

新型多齿多联氮杂环氮氧化物多氨基多羧基类稀土发光配合物及其在免疫分析中的应用
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    20761002
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    16.0 万元
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    地区科学基金项目

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EV-HIV 检测是一种即时横向流动免疫检测,可通过血液细胞外囊泡准确识别 HIV 感染
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