Antinociceptive Effect of Central Nerve System with Linear Polarized Near-infrared Light in a Chronic Pain Model

制作了被认为是RSD（反射性交感神经营养不良）动物模型的Bennett模型。在大鼠右侧坐骨神经周围松绑4条4-0铬肠缝合线。左侧坐骨神经没有结扎而是暴露在外。在辐射热试验中用热刺激评估痛感过敏，并使用von Frey头发进行压力刺激。辐射热试验：将大鼠置于玻璃板上。然后，用玻璃板下面的辐射热照射鞋底。后爪收回自动终止刺激并停止定时器。压力试验：将老鼠放在金属网上。从金属网下面，用von Frey发的压力刺激刺激鞋底。移动后爪的纤维的厚度是痛觉过敏的一个指标。选择术后1周出现高语速的大鼠。用线偏振近红外光单次照射延髓和大脑感觉区，观察其抗伤害感受作用。Bennett模型的痛觉过敏在8周后消失。选择术后1周出现阅读障碍的大鼠。我们每天用线偏振近红外光照射延髓附近一到三周。我们每天用光照射大脑感觉区域附近，持续一到三周。我们检验了它是否有恢复效果。Shimose制作动物模型，并照射线偏振近红外光。Muneshige做辐射热测试，Shimose做von Frey头发测试。预期结果：在RSD动物模型中，光照射延髓或大脑感觉区附近具有抗感觉性作用。这显示了光照射在人类大脑感觉区和延髓附近产生作用的可能性。

Bennett model which is regarded as an animal model of RSD (Reflex sympathetic dystrophy) is made. Four 4-0 chromic gut sutures were tied loosely around the right sciatic nerve of the rat. The left sciatic nerve is not ligated but exposed. The hyperalgesia is evaluated with the thermal stimulation in a radiant heat test, and is done with the pressure stimulation using a von Frey hair. Radiant heat test : The rat is put on the glass plate. Then, the sole is irradiated with radiant heat from under the glass plate. Hind paw withdrawal automatically terminated the stimulus and stop the timer. Pressure test : The rat is put on the metal mesh. From under the metal mesh, the sole is stimulated with the pressure stimulation using a von Frey hair. The thickness of the filament which moves the hind paw is an index of hyperalgesia. Rats that showed hyperlgesia one week postoperatively are chosen. Single-session irradiation with linear polarized near infrared light was made near the medulla oblongata and the cerebrum sensory area, and we observe the antinociceptive effect. The hyperalgesia of the Bennett model disappears about eight week later. Rats that showed hyperlgesia one week postoperatively were chosen. We irradiates every day near the medulla oblongata for one or three weeks with linear polarized near-infrared light. We irradiates every day near the cerebrum sensory area for one or three weeks with the light. We examined whether it has recovery effect or not. Shimose makes the animal model and irradiates linear polarized near-infrared light. Muneshige makes the radiant heat test, and Shimose does the von Frey hair test. The anticipating result : Irradiation near the medulla oblongata or cerebrum sensory area with the light showed antinociceptive effect in the animal model of RSD. This shows the possibility of the effect of the light irradiation near cerebrum sensory area and medulla oblongata in the human being.