肝癌におけるHGF activator inhibitor発現の意義

HGF激活剂抑制剂在肝癌中表达的意义

• 批准号: 11877092
• 负责人: 弘野 修一
• 金额: $ 1.09万
• 依托单位: 宮崎医科大学
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11877092/
• 关键词: HGF; HGF activator; HGF activator inhibitor; HCC; 肝硬変; HAI-1; 肝癌

HGFは一本鎖のプロHGFとして産生分泌され、傷害局所でセリンプロテアーゼであるHGFactivator(HA)により二本鎖の成熟型HGFとなり、活性を発現する。最近HGF activatorの作用を特異的に抑制するHGF activator inhibitor-1および2(HAI-1およびHAI-2)が単離され、cDNAがクローニングされた。HAは主として肝細胞で産生されており、HAI-1は胎盤、腎臓、膵臓、前立腺や消化管において発現が認められるが、肝臓では発現がみられない。種々の肝疾患におけるHAI-1の発現は検討されていないので、今回、我々は肝硬変(LC)を伴った原発性肝癌(HCC)16例、肝硬変6例および慢性肝炎(CH)9例の肝組織を用いてHAとHAI-1の免疫組織染色を行なった。その結果、HAの免疫染色では、CH、LCおよびHCCの患者肝組織において肝実質細胞が染色された。また肝癌組織でもHAは陽性であり、非癌部肝細胞とほぼ同様の染色性が認められた。一方、HAI-1は16例の肝癌組織中6例で陽性であり、CH、LCおよびHCCの非癌部組織には陽性細胞はみられなかった。また、AFP値が400ng/ml未満の肝癌ではHAI-1陰性が多く、400ng/ml以上の症例に陽性例が多かった。さらに一部の手術症例について癌部と非癌部に分けてRNAを抽出し、HAI-1のノーザンブロットを行ったところ、免疫組織化学で陽性の癌部においてHAI-1mRNAの発現が確認された。以上より、HAは肝細胞だけでなく肝癌組織にも発現しているが、HAI-1は肝癌組織特異的に発現していた。HAI-1陽性例はAFP高値の症例に多く、発癌や癌の浸潤にHAI-1が関連している可能性が考えられる。

HGF is a lock that produces secretion and damages the station. HGFactivator (HA) is a mature type of HGF that has two original locks and is active. Recently, HGF activator inhibitor-1 HGF activator inhibitor-1 HAI-1 HAI-2 (HAI-1 HAI-2) HGF activator specific inhibitor, cDNA HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator HAI-2 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 than HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor-1 HGF activator inhibitor does not work well. HA is mainly produced by liver cells, and HAI-1 is produced in placenta, kidney, and liver cells. The anterior prostate gland and the digestive tract are both open and closed, and the liver is open and closed. Kind of liver disease, HAI-1の発成は検问されていないので, this time, I have liver cirrhosis (LC), accompanied by primary liver cancer (H) Liver tissue of 16 cases of CC), 6 cases of cirrhosis and 9 cases of chronic hepatitis (CH) were immunohistochemically stained with HA and HAI-1. Results, HA immunostaining, CH, LC, HCC patient liver tissue staining, liver cytoplasmic cells staining. The liver cancer tissue is HA-positive and the non-cancerous liver cells are the same as the HA-stained ones. On the one hand, HAI-1 was detected in 6 out of 16 liver cancer tissues, CH, LC, and non-cancerous tissues in HCC were positive cells. There are many cases of HAI-1 negative liver cancer if AFP value is less than 400ng/ml, and there are many positive cases of AFP value above 400ng/ml. Examples of surgical procedures for one part of the disease: cancer part and non-cancerous part were divided, RNA was extracted, and HAI-1 was extracted.ットを行ったところ、Immunohistochemistry is positive and the cancer part is においてHAI-1mRNA is now confirmed. Above, HA is a hepatocyte-specific test for liver cancer tissue, and HAI-1 is a test for liver cancer tissue-specific test. There are many cases of HAI-1 positive cases, high AFP symptoms, and cases of cancer and infiltration of cancer, and the possibility of HAI-1 is not related to it.