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The molecular regulation of nuclear dynamics during cell cycle.

细胞周期中核动力学的分子调控。

基本信息

批准号：
11680693
负责人：
FURUKAWA Kazuhiro
金额：
$ 2.37万
依托单位：
NIIGATA UNIVERSITY (2000)Nagoya University (1999)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680693/
关键词：
nuclear structure nuclear envelope nuclear lamina LAP2 BAF L2BP1

项目摘要

Lamina-associated polypeptide (LAP) 2, which directly interacts with B-type lamins and chromosomes, is an integral membrane protein specifically distributed along the inner nuclear membrane of the nuclear envelope. The chromatin- and lamin- binding activity of LAP2 suggests that LAP2 plays an important role in targeting mitotic vesicles to chromosomes and reorganizing the nuclear structure at the end of mitosis.In the present study, in order to identify a protein with which the LAP2 protein interacts during nuclear architecture reformation, a yeast two-hybrid screen using the nucleoplasmic domain of LAP2 as a bait, was carried out. Here I identified a LAP2 interacting protein, termed L2BP1 (LAP2 binding protein 1). The rat L2BP1 cDNA sequence is predicted to encode a protein of 89 amino acids which turns out to be a rat homolog of mouse and human BAF (Barrier-to-Autointegration Factor). BAF/L2BP1 is distributed diffusely throughout the nucleus in interphase cells. It is, however, highly concentrated at the chromosomes during the M-phase. Further, the BAF/L2BP1 binding domain of LAP2 overlaps its chromosome-binding region. These findings suggest that BAF/L2BP1 is a candidate mediator of LAP2-chromosome interaction at the end of mitosis, and may participate as targeting sites on chromosomes for membrane traffic in the first step of nuclear architecture reformation.

核纤层相关多肽2（Lamina-associated polypeptide，LMP 2）是一种特异性分布于核被膜内的膜蛋白，与B型核纤层蛋白和染色体直接相互作用。染色质和核纤层蛋白的结合活性表明，LAP 2在有丝分裂结束时将有丝分裂囊泡靶向染色体和重组核结构中起着重要作用。在这里，我鉴定了一种LAP 2相互作用蛋白，称为L2 BP 1（LAP 2结合蛋白1）。大鼠L2 BP 1 cDNA序列预测编码89个氨基酸的蛋白质，其原来是小鼠和人BAF（自整合屏障因子）的大鼠同源物。BAF/L2 BP 1在间期细胞中弥散分布于整个细胞核。然而，在M期，它高度集中在染色体上。此外，LAP 2的BAF/L2 BP 1结合结构域与其染色体结合区域重叠。这些结果表明，BAF/L2 BP 1是一个候选调解员的LAP 2-染色体相互作用在有丝分裂结束，并可能参与作为靶位点的染色体上的膜交通的第一步，核结构改造。