Functional analysis of zebrafish retina-specific atonal gene and its expression profile

斑马鱼视网膜特异性无调性基因的功能分析及其表达谱

• 批准号: 11680729
• 负责人: MASAI Ichiro
• 金额: $ 2.37万
• 依托单位: The institute of physical and chemical research (RIKEN)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680729/
• 关键词: zebrafish; retina; neuronal differentiation; atonal; ゼブラフィッシュ

In the vertebrate, the retina region is specified in anterior neural plate and evaginates to form the optic cup, where six major classes of neurons and one-type of glia differentiate. Thus, the retina provides a good model, in which to study neuronal differentiation in the developing brain. Another interesting feature is that neuronal differentiation is initiated in one location and spreads to the whole region of the retina. In the compound eye of the invertebrate, the fruit fly, Drosophila melanogaster, cycle between activation of secretion factor, Hedgehog, and transcription factor, Atonal, is essential for progression of a neurogenic wave across the eye field. However, mechanisms underlying induction and progression of neurogenesis in the vertebrate retina is unknown.To elucidate mechanisms underlying induction and progression of neuronal differentiation in the developing retina, we cloned zebrafish atonal gene, ath5, which expresses exclusively in newly differentiating retinal neur … More ons, In zebrafish, ath5 expression is initiated at nasal retina and spreads to dorsal and temporal region of the retina, suggesting that a wave of neuronal differentiation spreads from nasal to temporal in zebrafish. The nasal region is adjacent to the optic stalk tissue, raising a possibility that interaction between optic stalk and neural retina is important for initial induction of ath5 expression in the nasal retina. To test this possibility, the boundary between the optic stalk and the neural retina was ablated with focussed laser beam. This ablation disrupts initial induction of ath5 expression. Furthermore, transplantation of optic stalk tissue induces ectopically ath5 expression in temporal region of the retina. These data suggest that initial induction of neurogenesis is regulated by signals from optic stalk and that progression process is mediated by relays of short-range signals. These data suggest a striking similarity of regulation of a neurogenic wave between the vertebrate and Drosophila eye. Less

在脊椎动物中，视网膜区域在前神经板中被指定并且外翻以形成视杯，其中六个主要类别的神经元和一种类型的神经胶质分化。因此，视网膜提供了一个很好的模型，在其中研究发育中的大脑中的神经元分化。另一个有趣的特征是神经元分化在一个位置开始并扩散到视网膜的整个区域。在复眼的无脊椎动物，果蝇，黑腹果蝇，周期之间的激活分泌因子，刺猬，和转录因子，无调，是必不可少的进展的神经性波的整个视野。然而，脊椎动物视网膜中神经发生的诱导和进展的机制尚不清楚，为了阐明发育中视网膜神经元分化的诱导和进展的机制，我们克隆了斑马鱼无调基因ath5，该基因仅在新分化的视网膜神经元中表达。 ...更多信息 在斑马鱼中，ath5表达起始于鼻侧视网膜，并扩散到视网膜的背侧和颞侧区域，这表明在斑马鱼中神经元分化的波从鼻侧扩散到颞侧。鼻区与视柄组织相邻，提示视柄和神经视网膜之间的相互作用对于鼻视网膜中ath5表达的初始诱导是重要的。为了测试这种可能性，用聚焦激光束烧蚀视柄和神经视网膜之间的边界。这种消融破坏了ath5表达的初始诱导。此外，视柄组织移植诱导了视网膜颞区ath5的异位表达。这些数据表明，神经发生的初始诱导是由视柄的信号调节，进展过程是由短程信号的中继介导的。这些数据表明，脊椎动物和果蝇眼睛之间的神经性波的调节惊人的相似性。少

项目成果

Masai, I., Stemple, D.L., Okamoto, H., and Wilson, S.W.: "Midline signals regulate retinal neurogenesis in zebrafish."Neuron. 27. 251-263 (2000)

Masai, I.、Stemple, D.L.、Okamoto, H. 和 Wilson, S.W.：“中线信号调节斑马鱼的视网膜神经发生。”神经元。

Masai,I.,Stemple,D.L.,Okamoto,H.,Wilson,S.W.: "Midline signals regulate retinal neurogenesis in zebrafish"Neuron. 27. 251-263 (2000)

Masai，I.，Stemple，D.L.，Okamoto，H.，Wilson，S.W.：“中线信号调节斑马鱼的视网膜神经发生”神经元。

政井一郎: "ゼブラフィッシュ網膜における神経細胞分化の開始および伝搬機構"蛋白質核酸酵素「小型魚類研究の新展開」. 54. 2782-2790 (2000)

马西一郎：“斑马鱼视网膜神经元分化的启动和传播机制”蛋白质核酸酶“小鱼研究的新进展”54。2782-2790（2000）。