Studies on diagnosis and pathogenicity of Pasteurella pneumotropica in mice.

小鼠嗜肺巴氏杆菌的诊断及致病性研究。

• 批准号: 11680826
• 负责人: KAWAMOTO Eiichi
• 金额: $ 1.66万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11680826/
• 关键词: Pasteurella pneumotopica; mouse; infectious disease; diagnosis; pathogenicity

The author focused this studies on transport medium for pasteurella pneumotropica and morphology of the bacteria. The ability of leibovitz medium no. 15 with 0.5% agar (L-15 transport medium), phosphate medium (0.3% disodium phosphate ), and Cary-Blair medium to preserve the viabilities of 3 P.pneumonia strains was studied. L-15 transport medium was found to be superior to the other media, enabling survival of organism for more 7 days at room temperature without decreasing the numbers of viable P.pneumotropica. The structures of cell surfaces with the strains of P.pneumonia from mouse and rat were studied by transmission electron microscopy with or without polycationic ferritin labeling. The 3 strains of P.pneumotropica, isolates of mouse, rat, and reference strain from mouse, ATCC 35149 , have characteristic cell surfaces of gram negative bacteria. There are differences between mouse and rat strains. Many bacteria cells of rat strain were dead, whereas those of mouse strains were good survived. Furthermore, biological and biochemical properties of the strains were investigated. All strains were identified as type Jawetz.

作者将这项研究重点放在用于肺炎巴氏氏菌的转运培养基和细菌的形态上。 Leibovitz培养基编号的能力。 15使用0.5％琼脂（L-15转运培养基），磷酸盐培养基（0.3％磷酸二钠）和Cary-Blair培养基保留3 p.pneumonia菌株的生存能力。发现L-15的传输培养基优于其他培养基，在室温下使生物体生存了7天，而无需减少可行的p.pneumotropica的数量。通过带有或没有多阳离子铁蛋白标记的透射电子显微镜研究了与小鼠和大鼠的细胞表面结构。 pneumotropica菌株的3菌株，小鼠的分离株，大鼠和参考菌株ATCC 35149具有革兰氏阴性细菌的特征细胞表面。小鼠和大鼠菌株之间存在差异。许多大鼠菌株的细菌细胞死亡，而小鼠菌株的细胞存活良好。此外，研究了菌株的生物学和生化特性。所有菌株均被识别为Jawetz型。

项目成果

Sawada,T.,Borrathybay,E.,Kawamoto,E.,et al.: "Fowl cholera in Japan : Disease occurrence and characteristics of Pasteurella multocida isolates"Bull. Nippon Vet. Anim. Sci. Univ.. 48. 21-32 (1999)

Sawada，T.，Borrathybay，E.，Kawamoto，E.，等人：“日本禽霍乱：多杀性巴氏杆菌分离株的疾病发生和特征”公牛。

Sawada,T.Borrathybay,E.,Kawamoto,E. et al.: "Fowl choleru in Japan : Disease occurrence and characterstics of Pasteurella multocida isolates"Bull.Nippon Vet.Anim.Sci.Univ.. 48. 21-32 (1999)

泽田，T.Borrathybay，E.，川本，E.

前島一淑 監修 有川二郎,石原智明,川本英一 他: "実験動物感染病の対応マニュアル"アドスリー. 345 (2000)

前岛和义有川次郎、石原智明、川本荣一等监修：《实验动物传染病处理手册》Ad345（2000）。

Sawada,T.,Borrathybay,E.,Kawamoto,E. et al.: "Fowl cholera in Japan : Disease occurrence and Characteristics of Pasteurella multocida isolates"Bull.Nippon Vet.Anim.Sci.Univ.. 48. 21-32 (1999)

Sawada，T.，Borrathybay，E.，Kawamoto，E.

Sawada, T., Borrathybay, E., Kawamoto, E., Koeda, T., and Ohta, S.: "Fowl cholera in Japan : Disease Occurrence and characteristics of Pasteurella multocida isolates."Bull. Nippon Vet. Anim. Sci. Univ.. No.48. 21-32 (1999)

Sawada, T.、Borrathybay, E.、Kawamoto, E.、Koeda, T. 和 Ohta, S.：“日本禽霍乱：多杀性巴氏杆菌分离株的疾病发生和特征。”公牛。