Studies on diagnosis and pathogenicity of Pasteurella pneumotropica in mice.

本文重点对嗜肺巴氏杆菌的运输介质和细菌形态进行了研究。本文研究了含0.5%琼脂的莱博维茨15号(L-15)、磷酸盐(0.3%磷酸二钠)和卡里-布莱尔(Cary-Blair)三种培养基对3株肺炎假单胞菌存活率的保存能力。结果表明，L-15运输培养基能在不降低嗜肺假单胞菌活菌数的情况下，使微生物在室温下存活7d以上。用透射电子显微镜观察了肺炎支原体感染小鼠和大鼠的细胞表面结构。从小鼠、大鼠分离的3株嗜肺假单胞菌和来自小鼠的参考菌株ATCC 35149具有革兰氏阴性菌的细胞表面特征。小鼠和大鼠品系之间存在差异。大鼠品系的细菌细胞大量死亡，而小鼠品系的细菌细胞存活良好。此外，还对菌株的生物学和生化特性进行了研究。所有菌株均为Jawetz型。

The author focused this studies on transport medium for pasteurella pneumotropica and morphology of the bacteria. The ability of leibovitz medium no. 15 with 0.5% agar (L-15 transport medium), phosphate medium (0.3% disodium phosphate ), and Cary-Blair medium to preserve the viabilities of 3 P.pneumonia strains was studied. L-15 transport medium was found to be superior to the other media, enabling survival of organism for more 7 days at room temperature without decreasing the numbers of viable P.pneumotropica. The structures of cell surfaces with the strains of P.pneumonia from mouse and rat were studied by transmission electron microscopy with or without polycationic ferritin labeling. The 3 strains of P.pneumotropica, isolates of mouse, rat, and reference strain from mouse, ATCC 35149 , have characteristic cell surfaces of gram negative bacteria. There are differences between mouse and rat strains. Many bacteria cells of rat strain were dead, whereas those of mouse strains were good survived. Furthermore, biological and biochemical properties of the strains were investigated. All strains were identified as type Jawetz.