Characterizing Emerging Humanized Immune Mouse Models for the study of transplant rejection and infectious disease pathology (Epstein Barr Virus)

本项目致力于人源化免疫系统(HIS)小鼠的直接比较和免疫学分析，这些小鼠来自胎儿和新生儿人体组织。新生儿HIS模型将得到优化，以更好地概括系统和组织特异性的人类先天和适应性免疫以及淋巴组织的发育。将开发两个新的人源化小鼠宿主菌株，并对它们在没有辐射的情况下产生强大的人类免疫细胞植入和功能的能力进行评估。开发的模型将通过研究T细胞介导的移植排斥反应和使用爱泼斯坦-巴尔病毒(EBV)检查传染病病理学来评估。马修·布朗博士将担任PI；这项工作将与马萨诸塞大学(Michael Brehm博士)和杰克逊实验室(Leonard Shultz博士)合作完成。他们之前拥有的冷冻保存的胎儿组织将用于这些研究。不会为这项研究获取新的胎儿组织。

This project focuses on direct comparisons and immunological analyses of humanized immune system (HIS) mice made from fetal vs neonatal human tissue sources. The neonatal HIS model will be optimized to better recapitulate systemic and tissue-specific human innate and adaptive immunity and lymphoid tissue development. Two novel humanized mouse host strains will be developed and evaluated for their ability to generate robust human immune cell engraftment and function without irradiation. The models developed will be assessed by studying T cell-mediated transplant allorejection responses and by examining infectious disease pathology using Epstein Barr Virus (EBV). Dr. Matthew Brown will serve as the PI; the work will be done in collaboration with the University of Massachusetts (Dr. Michael Brehm) and Jackson Laboratory (Dr. Leonard Shultz). Previously cryopreserved fetal tissue in their possession will be used for these studies. No new fetal tissue will be procured for this study.