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Dynamics of enteropathogenic bacteria in Asian environment

亚洲环境中肠道致病菌的动态

基本信息

批准号：
11691207
负责人：
NISHIBUCHI Mitsuaki
金额：
$ 7.06万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11691207/
关键词：
Asia Aquatic environment Seafood Vibrio parahaemolyticus Escherichia coli O157 Vibrio cholerae

项目摘要

We reported frequent infection due to a new clone of Vibrio parahaemolyticus in eight countries and the United States since 1996. It allowed us to use the term "pandemic." We initially defined the pandemic clone by its O3:K6 serovar, but we subsequently established a toxR-targeted PCR method to identify the pandemic clone. Recently, similar to the O3:K6 pandemic clone, serovariants of the pandemic clone have been spreading across the international border. We have been isolating the pandemic strains, including an O1:K25 strain, from the bivalves in southern Thailand since 1998, and we demonstrated strong association between environmental and clinical strains. The results indicated that improperly cooked bivalves are the means of transmission, and it may be the cause of international spread. Economic factor, ability to purchase expensive seafood, was a more important factor of infection than the poor hygienic condition in Vietnam.We demonstrated that first seafood marketed in Malaysia is frequently contaminated with O139 Vibrio cholerae, which indicates that Malaysian environment is contaminated with this microorganism. We also showed an epidemic due to tetracycline-resistant O1 V. cholerae for the first time in southern Thailand.These results suggest human movement in the southern Thailand-Malaysian region needs to be monitored.We reported contamination of the beef imported from India to Malysia and the beef and cattle feces in Thailand by Escherichia coli O157. We also reported distribution of vancomycin-resistant enterococci and Vibrio vulnificus in Malaysian environment.

我们报告自 1996 年以来在八个国家和美国频繁发生副溶血性弧菌新克隆感染。这使我们能够使用“大流行”一词。我们最初通过其 O3:K6 血清型定义了大流行克隆，但随后我们建立了一种以 toxR 为目标的 PCR 方法来识别大流行克隆。最近，与 O3:K6 大流行克隆类似，大流行克隆的血清变种已经跨越国际边境传播。自 1998 年以来，我们一直在泰国南部的双壳类动物中分离出大流行毒株，包括 O1:K25 毒株，并且我们证明了环境毒株和临床毒株之间的密切关联。结果表明，烹饪不当的双壳类是传播途径，可能是国际传播的原因。经济因素，购买昂贵海鲜的能力，是比越南恶劣的卫生条件更重要的感染因素。我们证明，在马来西亚销售的第一批海鲜经常受到O139霍乱弧菌的污染，这表明马来西亚的环境受到这种微生物的污染。我们还首次在泰国南部发现了由抗四环素的O1型霍乱弧菌引起的流行病。这些结果表明需要监测泰国南部-马来西亚地区的人类活动。我们报告了从印度进口到马来西亚的牛肉以及泰国的牛肉和牛的粪便被大肠杆菌O157污染。我们还报告了马来西亚环境中耐万古霉素肠球菌和创伤弧菌的分布。