Analysis and imaging of arrhythmia electrophysiological phenomena in the heart by modeling and experimental investigation of them

通过建模和实验研究对心脏心律失常电生理现象进行分析和成像

• 批准号: 12308046
• 负责人: INADA Hiroshi
• 金额: $ 21.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12308046/
• 关键词: Arrhythmia; Computer simulation; Spiral reentry; LR model; Optical mapping system of myocardial action potential; Response of whole heart to electrical stimuli; Two dimensional specimen of the parfused rabbit; Dynamics of spiral reentry; Siral Ree

In the present study we applied techniques of computer simulation to analyzing propagation of cardiac excitation wave at the occurrence of arrhythmia and its related phenomena and developed a system for measuring action potential of myocardial cell membrane. Then, the following investigations were conducted in order to compare observation results of spiral reentry generated by experiments with results of the simulation. (1) Simulation by using a model of myocardial action potential and visualization of propagation of cardiac excitation : In this study, one myocardial unit was expressed by the differential equation by using LR(Luo-Rudy) model and the simulation of arrhythmias was performed with a supercomputer. Secondly, we reproduced the propagation of cardiac excitation based on the theory of spiral wave and succeeded in visualization of the propagation.c Development of a optical mapping system of myocardial action potential and investigation of responses of a whole heart to electric … More stimuli by analyzes of optical signals : We developed a high-resolution optical mapping system with two high-speed video digital cameras to observe widely membrane action potential of a heart of a Japanese white rabbit which was dyed using the voltage sensitive dye di-4-ANEPPS. The responses of the whole heart to the electric stimuh can be investigated in detail by the analyzes of optical signals with the system. (3) Analysis of the relationship between dynamics of spiral reentry and the action potential using the perfused rabbit heart as materials and investigation of function of anti-arrhythmic drugs : By using the optical mapping system, the spiral reentry of two-dimensional specimen of the perfused rabbit heart is investigated from the viewpoint of dynamics of excitation rotation and changes in the wave shape of action potential and the function of anti-arrhythmic drugs. As the results, it was revealed that the dynamics of the spiral reentry was associated with interaction of the wave fronts and the tails of excitation, anisotropy of myocardial structure and so on. Less

本研究将计算机模拟技术应用于分析心律失常发生时心脏兴奋波的传播及相关现象，研制了一套心肌细胞膜动作电位测量系统。然后，进行了以下研究，以比较实验产生的螺旋再入观测结果和模拟结果。(1)心肌动作电位模型的模拟和心脏兴奋传播的可视化：本研究采用LR(Luo-Rudy)模型将一个心肌单位用微分方程式表示，并用超级计算机进行心律失常的模拟。其次，我们基于螺旋波理论再现了心脏兴奋的传播过程，并成功地对其传播进行了可视化。c.研制了心肌动作电位光学标测系统，并研究了整个心脏对电…的反应通过对光信号的分析获得更多的刺激：我们研制了一套高分辨率的光学标测系统，用两台高速摄像机对用电压敏感染料di-4-ANEPPS染色的日本大白兔心脏的膜动作电位进行了广泛的观察。利用该系统对光信号进行分析，可以详细地研究整个心脏对电刺激的响应。(3)以兔心脏灌流为材料，分析螺旋折返动力学与动作电位的关系及抗心律失常药物作用的研究：利用光学标测系统，从兴奋旋转动力学、动作电位波形变化及抗心律失常药物作用的角度，对二维标本的螺旋折返进行了研究。结果表明，螺旋折返的动力学特性与波头和激励尾部的相互作用、心肌结构的各向异性等因素有关。较少

项目成果

Ichiro Sakuma, Hiroshi Inada, Itsuo Kodama, et.al: "Computer Simulations of Electrical Propagation in Cardiac Tissue"Medical Physics. 27-6. (2000)

Ichiro Sakuma、Hiroshi Inada、Ituo Kodama 等人：“心脏组织中电传播的计算机模拟”医学物理学。

中沢一雄, 他: "R-on-TとPacing-on-Tでは心室受攻期が異なる:コンピュータシミュレーションによる研究"不整脈. 18(4). 496-503 (2002)

Kazuo Nakazawa 等人：“R-on-T 和 Pacing-on-T 之间的心室发作期不同：使用计算机模拟进行的研究”心律失常 18(4)。

Sakuma I, Kodama I, et al.: "Sustained monomorphic ventricular tachycardia in the two-dimensional left ventricular epicardial sheets of rabbit hearts perfused in-vitro"Environmental Medicine. 45. 92-94 (2001)

Sakuma I、Kodama I 等人：“体外灌注兔心脏二维左心室心外膜片中持续单形性室性心动过速”环境医学。

Kazuo Nakazawa, et al.: "Rotational Anisotropy Promotrs Breakthrough-Wave in Left Ventricle during Fibrillation : A Simulation Study"Journal of Cardicvascular Electrophysiology. 12・3(in press). (2001)

Kazuo Nakazawa 等人：“颤动期间左心室的旋转各向异性促进剂突破波：模拟研究”心血管电生理学杂志 12・3（出版中）。

Ichiro Sakuma, Itsuo Kodama, et al.: "Sustained monomorphic ventricular tachycardia in the two-dimensional left ventricular epicardial sheets of rabbit hearts perfused in-vitro"Environmental Medicine. 45. 92-94 (2001)

Ichiro Sakuma、Itsuo Kodama 等人：“体外灌注兔心脏二维左心室心外膜片中持续单形性室性心动过速”环境医学。