Development of novel humanized-mice and application to regenerative medicine

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Application of cells/tissues obtained from a human as a new therapy for the disease attracts attention. It seems to be logical that cells/tissues obtained from patients are used for therapy because there is not rejection. Therefore it is important that multiply various cells out of a body are induced into certain differentiation via a specific course. For developmental research of new medical material, we examined an introduction of differentiated cells derived from human bone marrow mesenchymal stem cells and inoculation of these human cells into immunodeficiency mice in this study.At first, the differentiation of cardiac muscle cells from human bone marrow mesenchymal stem cells which cells are easy to gather was performed. As a result, the method for differentiation into the cardiac muscle cells from human marrow mesenchymal stem cells was established. These obtained cardiac muscle cells may be useful for treatment of irreversible heart disorder such as myocardial infarction or cardiomyopathy. Furthermore it succeeds that the human cardiac muscle cells were transplanted into hearts of NOD/SCID. This experimental system may be available for preclinical trial of these muscle cells in vivo and evaluation of safety.The translantations of human bone or liver into NOD/SCID mice were examined. As a results, the reproduction of human bone remodeling, hemopoietic cells and blood vessels derived from human bone was observed. Human hepatic tissues also were maintained in murine tissues over 4 months. These humanized mice (SCID-hu) may be available for drug screening, preclinical trials of novel therapies including gene therapy or molecular targeting therapy, or in vivo analysis in molecular pathology.