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Cultivation of diatoms using Tb-doped silicate minerals to characterize silicon utilized by diatoms

使用掺铽硅酸盐矿物培养硅藻以表征硅藻利用的硅

基本信息

批准号：
12640477
负责人：
AKAGI Tasuku
金额：
$ 1.73万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

Diatoms are responsible for as much as 70% of the primary product in the ocean and play a major role of biological pump. This is because they sequester the atmospheric carbon dioxide effectively by transporting assimilated carbon into deeper layer of the ocean in capsules of relatively stable silica. However, the clear explanation for the timing of diatom bloom has not been given. We have conducted cultivation experiments of diatoms to investigate the chemical form of silicon incorporated by diatoms. So far, we have made the following achievements itemized below.1) Cultivation and analysisProliferation was observed when quartz particles were exclusive source of silicon and Similar proliferation was observed in the terbium doped system. The silicon particles, diatoms and seawater were successfully separated with two filters and the determination of silicon in quartz particles and diatoms was also successful with boiling the separated samples in a sodium hydroxide solution.2) Cultivation apparatusA wave-generator was designed and set in a controlled cultivation box to mimic the natural environment of diatom growth.3) The suppression of blank value of silicon in the analysisReference cultivations should be conducted at the comparatively small level of silicon concentration, which was made possible by selecting and washing proper equipments and plastic-ware.4) Time and condition of the diatom cultivationProliferation of diatoms was suppressed with rocking and the proliferation of diatoms were observed between two weeks and one month times.5) Incorporation of terbium in diatomsDetectable amount of terbium was incorporated in diatoms, suggesting that the diatoms utilized silicon in quartz particles. However, it was found that some replicate expriments are required to confirm the results.

硅藻负责海洋中多达70%的初级产品，并发挥生物泵的主要作用。这是因为它们通过将同化的碳以相对稳定的二氧化硅胶囊形式输送到海洋深层，有效地隔离了大气中的二氧化碳。然而，硅藻水华发生的时间并没有得到明确的解释。我们进行了硅藻的培养实验，以研究硅藻中硅的化学形式。1）培养与分析在石英颗粒为硅的唯一来源时观察到增殖，在铽掺杂体系中观察到类似的增殖。硅颗粒，利用两种滤膜成功地分离了硅藻和海水，并将分离后的样品在氢氧化钠溶液中煮沸，成功地测定了石英颗粒和硅藻中的硅。2）培养装置设计了一个波发生器，并将其安装在可控培养箱中，以模拟硅藻生长的自然环境。3）分析中硅空白值的抑制参比培养应在相对较小的硅浓度水平下进行，通过选择和清洗合适的设备和塑料器皿，硅藻培养的时间和条件通过摇动抑制硅藻的增殖，并在两周到一个月的时间内观察硅藻的增殖。5）铽在硅藻中的掺入可检测量的铽掺入到硅藻中，这表明硅藻利用石英颗粒中的硅。然而，发现需要一些重复实验来确认结果。