Microscopic Properties on the Structure of Biomolecules in Aqueous Solutions

水溶液中生物分子结构的微观特性

• 批准号: 12640534
• 负责人: KAMEDA Yasuo
• 金额: $ 1.66万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640534/
• 关键词: biomolecules; isotopic substitution method; neutron diffraction; X-ray diffraction; amino acid solutions; urea molecules; hydration structure; intermolecular hydrogen bonds; 中性子回折; X線回折; アンモニウムイオン; グリシン分子

In order to investigate microscopic structure of biomolecules in aqueous solutions, following structural analyzes have been carried out in this project;1) Hydration structure of ammonium ions studied by neutron diffraction with ^<14>N/^<15>N and H/D isotopic substitution methods.2) Hydration structure of urea molecules studied by neutron diffraction with ^<14>N/^<15>N and H/D isotopic substitution methods.3) Hydration structure of alanine molecules studied by neutron diffraction with ^<14>N/^<15>N and H/D isotopic substitution methods.4) Hydration structure of glycine molecules in aqueous acidic- and alkaline solutions studied by neutron diffraction with ^<14>N/^<15>N and H/D isotopic substitution methods.5) Hydration structure of acetate ions studied by X-ray and neutron diffraction techniques.A new X-ray imaging plate holder has been constructed, which enable to obtain scattering data with higher statistical accuracy. Measurements on standard samples have been completed. Refinements of the instrumental parameters and the program system for data analysis, are now in progress.

为了研究水溶液中生物分子的微观结构，本项目进行了以下结构分析：1)用中子衍射法研究了氨离子的水合结构，用中子衍射法研究了氨离子的水合结构；2)用中子衍射法研究了尿素分子的水化结构，用中子衍射法研究了尿素分子的水合结构。N和H/D同位素替代方法。4)用中子衍射法研究了甘氨酸分子在酸性和碱性水溶液中的水化结构。5)用X射线和中子衍射技术研究了醋酸根离子的水化结构。已完成对标准样品的测量。目前正在改进仪器参数和数据分析程序系统。

项目成果

H. Naganuma et al.: "Neutron and X-ray Diffraction Studies on the Structure of Concentrated Aqueous Sodium Acetate Solutions"Journal of Physical Society of Japan. 70, Supplement A. 356-358 (2001)

H. Naganuma 等人：“浓乙酸钠水溶液结构的中子和 X 射线衍射研究”日本物理学会杂志。

Y.Kameda et al.: "Structure of Concentrated Aqueous NH_4Cl Solutions Studied by Neutron Diffraction with ^<14>N^<15>N and H/D Isotopic Substitution Technique"Journal of the Physical Society of Japan, Supplement A. 70. 362-364 (2001)

Y.Kameda等人：“Structure of Concentrate Aqueous NH_4Cl Solutions Studied by Neutron Diffraction with ^<14>N^<15>N and H/D Isotopic Substitution Technique”日本物理学会杂志，增刊A.70。

M.Imano et al.: "Hydrogen-bonded Structure in Highly Concentrated Aqueous LiBr Solutions"Journal of the Physical Society of Japan, Supplement A. 70. 368-370 (2001)

M.Imano等人：“高浓度溴化锂水溶液中的氢键结构”日本物理学会杂志，增刊A.70.368-370(2001)

Y. Kameda et al.: "Structure of Concentrated Aqueous NH_4Cl Solutions Studies by Neutron Diffraction with ^<14>N/^<15>N and H/D Isotopic Substitution Technique"Journal of Physical Society of Japan. 70, Supplement A. 362-364 (2001)

Y. Kameda等人：“利用^ 14 N/^ 15 N和H/D同位素取代技术的中子衍射研究浓NH_4Cl溶液的结构”日本物理学会杂志。

K. Mochiduki et al.: "Hydration Structure of the Potassium Ion Determined by Combined Analysis of Neutron and X-ray Diffraction Data"Journal of Physical Society of Japan. 70, Supplement A. 371-373 (2001)

K. Mochiduki 等人：“通过中子和 X 射线衍射数据的联合分析确定钾离子的水合结构”日本物理学会杂志。