Development of a device for analyzing fine particles by exploiting acoustic radiation force and electrostatic force

作为开发分析生物细胞中各种成分的装置的第一步，根据前人的研究，组装了一种利用声辐射力浓缩液体中纳米颗粒的装置，并用视频显微镜观察了颗粒的分布。考察了颗粒浓度、颗粒在液体中的密度匹配、颗粒直径、通道内壁涂层以及超声峰峰值等参数。不幸的是，该装置的工作方式是不可重现的，没有得到可靠的结果。此外，通道的化学状态在这种器件的设计中起着重要的作用，因为它会影响表面产生的电渗透的大小和分离能力。硅酸盐表面以锐钛矿晶型的形式被覆盖了二氧化钛。根据溶液的pH值和组成，生成的表面有阳极或阴极的电渗作用。二氧化钛表面很容易被硅烷化试剂修饰，从而发生一定的层析作用。

As the first step toward developing a device by which various constituents in a biological cell are analyzed, a device for concentrating nanoparticles in liquid by acoustic radiation force was assembled according previous studies, and particle distribution was observed by video microscopy. Several variables such as particle concentration, density matching of particles in liquid, particle diameter, inner wall coating of channels, and peak-to-peak amplitude of ultrasound were examined. Unfortunately, the device works in an irreproducible manner and reliable results were not obtained. Apart from this, the chemical state of the channels plays an important part in the design of such a device, because it can affect the magnitude of electroosmosis generated on the surface as well as the separation ability.Silica surface was coated with titanium dioxide in a crystalline form of anatase. The resultant surface had anodic or cathodic electroosmosis, depending on the pH and composition of the solution. Titanium dioxide surface was readily modified by a silanizing reagent so that some chromatographic interaction took place.