Studies in the distribution of yieldin in cowpea seedlings

我们用抗高产素抗体检测了高产素在黄化豇豆幼苗中的分布和免疫组化定位。免疫印迹分析显示，产率蛋白位于胚珠、上胚轴和下胚轴上，而不在根上。下胚轴的产量信号强度在顶端预伸长区（钩形区）最高，向伸长的成熟基部逐渐减弱，表明产量信号随着细胞伸长的停止而消失。利用不同萌发阶段的下胚轴进行的组织打印免疫印迹分析支持了这一观点，因为顶端高产区，就在子叶结的下方（钩形区和快速伸长区），随着下胚轴伸长一起向顶端迁移。免疫组织化学显微镜观察发现，产素定位于胚轴皮层和表皮的细胞壁。本研究结果与产量因子参与伸长生长过程中细胞壁屈服调节的观点一致。

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation.Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.