Studies in the distribution of yieldin in cowpea seedlings

豇豆幼苗产量分布研究

• 批准号: 12640632
• 负责人: KATOU Kiyoshi
• 金额: $ 0.96万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640632/
• 关键词: elongation growth; cellwall; immunofluorescent microscopy; tissue printing; Wfestern blot; anti-yieldin antibody; yield threshold; Vigna unguiculata; 胚軸; 細胞壁伸展; yieldin; 免疫蛍光染色; 組織内分布

We examined the distribution and the immunohistochemical localization of yieldin in etiolated cowpea seedlings with an anti-yieldin antibody. An immunoblotting analysis revealed that the yieldin was located in the aerial organs (plumule, epicotyl and hypocotyl) but not in the roots. The intensity of the yieldin signal in the hypocotyls was highest in the apical pre-elongation region (the hook region) and decreased toward the elongated mature base indicating that the yieldin disappeared with the ceasing of cell elongation. Tissue-print immunoblotting analysis using hypocotyls in different germination stages supports this view because the apical yieldin-rich regions, just beneath the cotyledonary node (the hook and rapidly elongating regions), acropetally migrated together with hypocotyl elongation.Immunohistochemical microscopy demonstrated that yieldin was localized in the cell walls of the cortex and epidermis of the germ axes. The present results are consistent with the view that yieldin participates in the regulation of cell wall yielding during elongation growth.

我们用抗yieldin抗体研究了yieldin在黄化豇豆幼苗中的分布和免疫组织化学定位。免疫印迹分析表明，yieldin定位在地上器官（胚芽，上胚轴和下胚轴），而不是在根中。下胚轴中yieldin信号的强度在顶端伸长前区域（钩状区域）最高，并向伸长的成熟基部降低，表明yieldin随着细胞伸长的停止而消失。组织印迹免疫印迹分析支持这一观点，因为顶端yieldin丰富的地区，下面的子叶节（钩和快速伸长区），acropetally迁移与下胚轴elongation.Immunohistochemical显微镜表明，yieldin是本地化的皮层和表皮的细胞壁的胚芽轴。这些结果与yieldin参与了伸长生长过程中细胞壁屈服的调节的观点是一致的。

项目成果

Takahashi K. et al.: "Yieldin, a regulatory protein of yield threshold tension of plant cell walls"Kagaku to Seibutsu. 39(in japanese). 9-10 (2001)

Takahashi K.等人：“Yieldin，植物细胞壁屈服阈值张力的调节蛋白”Kagaku to Seibutsu。

高橋宏二 他: "植物細胞壁の臨界降伏張力を制御するタンパク質yieldin"化学と生物. 39. 9-10 (2001)

Koji Takahashi 等人：“Yieldin，一种控制植物细胞壁临界屈服张力的蛋白质”，化学与生物学，39. 9-10 (2001)。

Okamoto-N. A. et al.: "A regulatory protein of elongation growth and the physical property of plant cell walls"Chemical regulation of plants. 35(in japanese). 25-34 (2000)

冈本-N。

Okamoto-N.A. et al.: "Distribution of yleldin, a regulatory protein of the cell wall yield threshold, in etlolated cowpea seedlings"Plant Cell Physiol.. 42. 952-958 (2001)

冈本-N.A.

高橋宏二 他: "植物細胞壁の臨界降伏張力を調節するタンパク質yieldin"化学と生物. 39・1. 9-10 (2001)

Koji Takahashi 等：“Yieldin，一种调节植物细胞壁临界屈服张力的蛋白质”，化学与生物学 39・1（2001）。